Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A sheath of motile cells supports collective migration in of the Zebrafish posterior lateral line primordium under the skin

Damian Dalle Nogare, Naveen Natesh, Ajay Chitnis
doi: https://doi.org/10.1101/783043
Damian Dalle Nogare
1Section on Neural Developmental Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: damiandn@gmail.com
Naveen Natesh
1Section on Neural Developmental Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ajay Chitnis
1Section on Neural Developmental Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

Abstract

During embryonic development, cells must navigate through diverse three-dimensional environments robustly and reproducibly. The zebrafish posterior lateral line primordium (PLLp), a group of approximately 120 cells which migrates from the otic vesicle to the tip of the tail, spearheading the development of the lateral line sensory system, is an excellent model to study such collective migration in an in vivo context. This system migrates in a channel formed by the underlying horizontal myoseptum and somites, and the overlying skin. While cells in the leading part of the PLLp are flat and have a more mesenchymal morphology, cells in the trailing part progressively reorganize to form epithelial rosettes, called protoneuromasts. These epithelial cells extend basal cryptic lamellipodia in the direction of migration in response to both chemokine and FGF signals. In this study, we show that, in addition to these cryptic lamellipodia, the core epithelial cells are in fact surrounded by a population of motile cells which extend actin-rich migratory processes apposed to the overlying skin. These thin cells wrap around the protoneuromasts, forming a continuous sheath of cells around the apical and lateral surface of the PLLp. The processes extended by these cells are highly polarized in the direction of migration and this directionality, like that of the basal lamellipodia, is dependent on FGF signaling. Consistent with interactions of sheath cells with the overlying skin contributing to migration, removal of the skin stalls migration. However, this is accompanied by some surprising changes. There is a profound change in the morphology of the sheath cells, with directional superficial lamellipodia being replaced with the appearance of undirected blebs or ruffles. Furthermore, removal of the skin not only affects underlying lamellipodia, it simultaneously alters the morphology and behavior of the deeper basal cryptic lamellipodia, even though these cells do not directly contact the skin. Directional actin-rich protrusions on both the apical and basal surface and migration are completely and simultaneously restored upon regrowth of the skin over the PLLp. We suggest that this system utilizes a circumferential sheath of motile cells to allow the internal epithelial cells to migrate collectively in the confined space of the horizontal myopseptum and that elastic confinement provided by the overlying skin is essential for effective collective migratory behavior of primordium cells.

Footnotes

  • Updated details on transgenic line used and added additional acknowledgements

  • https://github.com/chitnislabnih/dallenogare2019

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
Back to top
PreviousNext
Posted October 01, 2019.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A sheath of motile cells supports collective migration in of the Zebrafish posterior lateral line primordium under the skin
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A sheath of motile cells supports collective migration in of the Zebrafish posterior lateral line primordium under the skin
Damian Dalle Nogare, Naveen Natesh, Ajay Chitnis
bioRxiv 783043; doi: https://doi.org/10.1101/783043
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
A sheath of motile cells supports collective migration in of the Zebrafish posterior lateral line primordium under the skin
Damian Dalle Nogare, Naveen Natesh, Ajay Chitnis
bioRxiv 783043; doi: https://doi.org/10.1101/783043

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4840)
  • Biochemistry (10768)
  • Bioengineering (8026)
  • Bioinformatics (27234)
  • Biophysics (13949)
  • Cancer Biology (11099)
  • Cell Biology (16021)
  • Clinical Trials (138)
  • Developmental Biology (8764)
  • Ecology (13258)
  • Epidemiology (2067)
  • Evolutionary Biology (17334)
  • Genetics (11673)
  • Genomics (15898)
  • Immunology (11009)
  • Microbiology (26024)
  • Molecular Biology (10620)
  • Neuroscience (56423)
  • Paleontology (417)
  • Pathology (1729)
  • Pharmacology and Toxicology (2999)
  • Physiology (4538)
  • Plant Biology (9613)
  • Scientific Communication and Education (1611)
  • Synthetic Biology (2677)
  • Systems Biology (6965)
  • Zoology (1508)