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Molecular Atlas of the Adult Mouse Brain

Cantin Ortiz, Jose Fernandez Navarro, Aleksandra Jurek, Antje Märtin, Joakim Lundeberg, View ORCID ProfileKonstantinos Meletis
doi: https://doi.org/10.1101/784181
Cantin Ortiz
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
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Jose Fernandez Navarro
Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden
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Aleksandra Jurek
Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden
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Antje Märtin
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
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Joakim Lundeberg
Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden
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  • For correspondence: dinos.meletis@ki.se joakim.lundeberg@kth.se
Konstantinos Meletis
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
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  • ORCID record for Konstantinos Meletis
  • For correspondence: dinos.meletis@ki.se joakim.lundeberg@kth.se
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Abstract

Brain maps are essential for integrating information and interpreting the structure-function relationship of circuits and behavior. We aimed to generate a systematic classification of the adult mouse brain organization based on unbiased extraction of spatially-defining features. Applying whole-brain spatial transcriptomics, we captured the gene expression signatures to define the spatial organization of molecularly discrete subregions. We found that the molecular code contained sufficiently detailed information to directly deduce the complex spatial organization of the brain. This unsupervised molecular classification revealed new area- and layer-specific subregions, for example in isocortex and hippocampus, and a new division of striatum. The whole-brain molecular atlas further supports the identification of the spatial origin of single neurons using their gene expression profile, and forms the foundation to define a minimal gene set - a brain palette – that is sufficient to spatially annotate the adult brain. In summary, we have established a new molecular atlas to formally define the identity of brain regions, and a molecular code for mapping and targeting of discrete neuroanatomical domains.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 27, 2019.
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Molecular Atlas of the Adult Mouse Brain
Cantin Ortiz, Jose Fernandez Navarro, Aleksandra Jurek, Antje Märtin, Joakim Lundeberg, Konstantinos Meletis
bioRxiv 784181; doi: https://doi.org/10.1101/784181
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Molecular Atlas of the Adult Mouse Brain
Cantin Ortiz, Jose Fernandez Navarro, Aleksandra Jurek, Antje Märtin, Joakim Lundeberg, Konstantinos Meletis
bioRxiv 784181; doi: https://doi.org/10.1101/784181

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