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Comparative single-cell transcriptomics of complete insect nervous systems

View ORCID ProfileB.T. Cocanougher, View ORCID ProfileJ.D. Wittenbach, View ORCID ProfileX. Long, A.B. Kohn, T.P. Norekian, J. Yan, J. Colonell, View ORCID ProfileJ.-B. Masson, J.W. Truman, View ORCID ProfileA. Cardona, S. Turaga, View ORCID ProfileR.H. Singer, View ORCID ProfileL.L. Moroz, View ORCID ProfileM. Zlatic
doi: https://doi.org/10.1101/785931
B.T. Cocanougher
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
2Department of Zoology, Cambridge University, Cambridge, UK
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J.D. Wittenbach
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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X. Long
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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A.B. Kohn
3Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida, USA
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T.P. Norekian
3Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida, USA
4Friday Harbor Laboratories, University of Washington, Friday Harbor, Washington, USA
5Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia
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J. Yan
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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J. Colonell
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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J.-B. Masson
7Decision and Bayesian Computation, CNRS UMR 3571, Neuroscience and Computational Biology Department, Institut Pasteur, Paris, France
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  • ORCID record for J.-B. Masson
J.W. Truman
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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A. Cardona
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
6Department of Physiology, Development, and Neuroscience, Cambridge University, Cambridge, UK
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S. Turaga
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
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R.H. Singer
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
8Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA
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L.L. Moroz
3Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida, USA
4Friday Harbor Laboratories, University of Washington, Friday Harbor, Washington, USA
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  • For correspondence: moroz@whitney.ufl.edu zlaticm@janelia.hhmi.org
M. Zlatic
1Howard Hughes Medical Institute Janelia Research Campus, Ashburn, Virginia, USA
2Department of Zoology, Cambridge University, Cambridge, UK
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  • For correspondence: moroz@whitney.ufl.edu zlaticm@janelia.hhmi.org
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Abstract

Molecular profiles of neurons influence information processing, but bridging the gap between genes, circuits and behavior has been very difficult. Furthermore, the behavioral state of an animal continuously changes across development and as a result of sensory experience. How behavioral state influences molecular cell state is poorly understood. Here we present a complete atlas of the Drosophila larval nervous system. We develop polyseq, a python analysis package, and use single molecule RNA-FISH to validate our scRNAseq findings. To investigate how internal state affects cell state, we altered internal state with high-throughput behaviour protocols designed to mimic wasp sting and over activation of the memory system. We found nervous system-wide gene expression changes related to cell state. This work advances our understanding of how genes, neurons, and circuits generate behavior.

One Sentence Summary Single cell genomics and modulation of internal state reveal how genes, neurons, and circuits produce behavior.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 30, 2019.
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Comparative single-cell transcriptomics of complete insect nervous systems
B.T. Cocanougher, J.D. Wittenbach, X. Long, A.B. Kohn, T.P. Norekian, J. Yan, J. Colonell, J.-B. Masson, J.W. Truman, A. Cardona, S. Turaga, R.H. Singer, L.L. Moroz, M. Zlatic
bioRxiv 785931; doi: https://doi.org/10.1101/785931
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Comparative single-cell transcriptomics of complete insect nervous systems
B.T. Cocanougher, J.D. Wittenbach, X. Long, A.B. Kohn, T.P. Norekian, J. Yan, J. Colonell, J.-B. Masson, J.W. Truman, A. Cardona, S. Turaga, R.H. Singer, L.L. Moroz, M. Zlatic
bioRxiv 785931; doi: https://doi.org/10.1101/785931

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