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ATP-driven separation of liquid phase condensates in bacteria

B. Guilhas, J.C. Walter, J. Rech, G. David, N.-O. Walliser, J. Palmeri, C. Mathieu-Demaziere, A. Parmeggiani, J.Y. Bouet, A. Le Gall, View ORCID ProfileM. Nollmann
doi: https://doi.org/10.1101/791368
B. Guilhas
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
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J.C. Walter
2Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
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J. Rech
3LMGM, CBI, CNRS, Univ. Toulouse, UPS, Toulouse, France
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G. David
2Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
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N.-O. Walliser
2Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
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J. Palmeri
2Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
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C. Mathieu-Demaziere
3LMGM, CBI, CNRS, Univ. Toulouse, UPS, Toulouse, France
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A. Parmeggiani
2Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
4LPHI, CNRS, Univ. Montpellier, Montpellier, France
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J.Y. Bouet
3LMGM, CBI, CNRS, Univ. Toulouse, UPS, Toulouse, France
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A. Le Gall
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
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M. Nollmann
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
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  • ORCID record for M. Nollmann
  • For correspondence: marcnol@gmail.com
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Abstract

Liquid-liquid phase separated (LLPS) states are key to compartmentalise components in the absence of membranes, however it is unclear whether LLPS condensates are actively and specifically organized in the sub-cellular space and by which mechanisms. Here, we address this question by focusing on the ParABS DNA segregation system, composed of a centromeric-like sequence (parS), a DNA-binding protein (ParB) and a motor (ParA). We show that parS-ParB associate to form nanometer-sized, spherical droplets. ParB molecules diffuse rapidly within the nucleoid volume, but display confined motions when trapped inside ParB droplets. Single ParB molecules are able to rapidly diffuse between different droplets, and droplet nucleation is strongly favoured by parS. Notably, the ParA motor is required to prevent the fusion of ParB droplets. These results describe a novel active mechanism that splits, segregates and localises LLPS condensates in the sub-cellular space.

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Posted October 02, 2019.
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ATP-driven separation of liquid phase condensates in bacteria
B. Guilhas, J.C. Walter, J. Rech, G. David, N.-O. Walliser, J. Palmeri, C. Mathieu-Demaziere, A. Parmeggiani, J.Y. Bouet, A. Le Gall, M. Nollmann
bioRxiv 791368; doi: https://doi.org/10.1101/791368
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ATP-driven separation of liquid phase condensates in bacteria
B. Guilhas, J.C. Walter, J. Rech, G. David, N.-O. Walliser, J. Palmeri, C. Mathieu-Demaziere, A. Parmeggiani, J.Y. Bouet, A. Le Gall, M. Nollmann
bioRxiv 791368; doi: https://doi.org/10.1101/791368

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