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A cost-efficient open source laser engine for microscopy

View ORCID ProfileDaniel Schröder, View ORCID ProfileJoran Deschamps, View ORCID ProfileAnindita Dasgupta, View ORCID ProfileUlf Matti, View ORCID ProfileJonas Ries
doi: https://doi.org/10.1101/796482
Daniel Schröder
1EMBL, Cell Biology and Biophysics, Meyerhofstr. 1, 69117 Heidelberg, Germany
2Current affiliation: Institute of Applied Optics and Biophysics, Friedrich Schiller University, Philosophenweg 7, 07743 Jena, Germany
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Joran Deschamps
1EMBL, Cell Biology and Biophysics, Meyerhofstr. 1, 69117 Heidelberg, Germany
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Anindita Dasgupta
1EMBL, Cell Biology and Biophysics, Meyerhofstr. 1, 69117 Heidelberg, Germany
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Ulf Matti
1EMBL, Cell Biology and Biophysics, Meyerhofstr. 1, 69117 Heidelberg, Germany
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Jonas Ries
1EMBL, Cell Biology and Biophysics, Meyerhofstr. 1, 69117 Heidelberg, Germany
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  • For correspondence: jonas.ries@embl.de
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Abstract

Scientific-grade lasers are costly components of modern microscopes. For high-power applications, such as single-molecule localization microscopy, their price can become prohibitive. Here, we present an open-source high-power laser engine that can be built for a fraction of the cost. It uses affordable, yet powerful laser diodes at wavelengths of 405 nm, 488 nm and 640 nm and optionally a 561 nm diode-pumped solid-state laser. The light is delivered to the microscope via an agitated multimode fiber in order to suppress speckles. We provide the part lists, CAD files and detailed descriptions, allowing any research group to build their own laser engine.

Footnotes

  • ↵3 jonas.ries{at}embl.de

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted October 15, 2019.
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A cost-efficient open source laser engine for microscopy
Daniel Schröder, Joran Deschamps, Anindita Dasgupta, Ulf Matti, Jonas Ries
bioRxiv 796482; doi: https://doi.org/10.1101/796482
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A cost-efficient open source laser engine for microscopy
Daniel Schröder, Joran Deschamps, Anindita Dasgupta, Ulf Matti, Jonas Ries
bioRxiv 796482; doi: https://doi.org/10.1101/796482

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