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Homeostatic regulation of perisynaptic MMP9 activity in the amblyopic visual cortex

View ORCID ProfileSachiko Murase, View ORCID ProfileDaniel E. Winkowski, Ji Liu, Patrick O. Kanold, View ORCID ProfileElizabeth M. Quinlan
doi: https://doi.org/10.1101/796623
Sachiko Murase
Department of Biology and Neuroscience and Cognitive Sciences Program University of Maryland, College Park, MD 20742
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Daniel E. Winkowski
Department of Biology and Neuroscience and Cognitive Sciences Program University of Maryland, College Park, MD 20742
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Ji Liu
Department of Biology and Neuroscience and Cognitive Sciences Program University of Maryland, College Park, MD 20742
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Patrick O. Kanold
Department of Biology and Neuroscience and Cognitive Sciences Program University of Maryland, College Park, MD 20742
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Elizabeth M. Quinlan
Department of Biology and Neuroscience and Cognitive Sciences Program University of Maryland, College Park, MD 20742
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  • ORCID record for Elizabeth M. Quinlan
  • For correspondence: equinlan@umd.edu
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Abstract

Dark exposure (DE) followed by light reintroduction (LRx) reactivates robust synaptic plasticity in adult mouse V1, which allows recovery from amblyopia. Previously we showed that LRx-induced perisynaptic proteolysis of extracellular substrates by MMP9 mediates the enhanced plasticity in binocular adult mice (Murase et al., 2017). However, it is unknown if a visual system compromised by amblyopia could engage this pathway. Here we show that LRx to adult amblyopic mice induces perisynaptic MMP2/9 activity and degradation of ECM in the deprived and non-deprived V1. LRx restricted to the amblyopic eye induces equally robust MMP2/9 activity at thalamo-cortical synapses and ECM degradation in deprived V1. Two-photon live imaging demonstrates that the history of visual experience regulates MMP2/9 activity in V1, and that DE lowers the threshold for the proteinase activation. This homeostatic reduction of MMP2/9 activation threshold by DE enables the visual input from the amblyopic pathway to trigger robust perisynaptic proteolysis.

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Posted October 10, 2019.
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Homeostatic regulation of perisynaptic MMP9 activity in the amblyopic visual cortex
Sachiko Murase, Daniel E. Winkowski, Ji Liu, Patrick O. Kanold, Elizabeth M. Quinlan
bioRxiv 796623; doi: https://doi.org/10.1101/796623
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Homeostatic regulation of perisynaptic MMP9 activity in the amblyopic visual cortex
Sachiko Murase, Daniel E. Winkowski, Ji Liu, Patrick O. Kanold, Elizabeth M. Quinlan
bioRxiv 796623; doi: https://doi.org/10.1101/796623

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