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Embryonic organoids recapitulate early heart organogenesis

Giuliana Rossi, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Matthias P. Lutolf
doi: https://doi.org/10.1101/802181
Giuliana Rossi
1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Vaud, Switzerland
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Andrea Boni
2Viventis Microscopy Sàrl, EPFL Innovation Park, building C, Lausanne, 1015, Vaud, Switzerland
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Romain Guiet
3Faculté des sciences de la vie, Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne (EPFL), Bâtiment AI, Station 15, Lausanne, 1015, Vaud, Switzerland
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Mehmet Girgin
1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Vaud, Switzerland
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Robert G. Kelly
4Aix-Marseille Université, CNRS UMR 7288, IBDM, Marseille, France
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Matthias P. Lutolf
1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Vaud, Switzerland
5Institute of Chemical Sciences and Engineering, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Vaud, Switzerland
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  • For correspondence: matthias.lutolf@epfl.ch
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Abstract

Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here we show that mouse embryonic stem cells (mESCs) can be coaxed to robustly undergo the fundamental steps of early heart organogenesis with an in vivo-like spatiotemporal fidelity. These axially patterned embryonic organoids support the generation of cardiovascular progenitors, as well as first and second heart field compartments. The cardiac progenitors self-organize into an anterior domain reminiscent of a cardiac crescent before forming a beating cardiac tissue near a putative primitive gut-like tube, from which it is separated by an endocardial-like layer. These findings unveil the surprising morphogenetic potential of mESCs to execute key aspects of organogenesis through the coordinated development of multiple tissues. This platform could be an excellent tool for studying heart development in unprecedented detail and throughput.

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  • Corrected first author name

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 17, 2019.
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Embryonic organoids recapitulate early heart organogenesis
Giuliana Rossi, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Matthias P. Lutolf
bioRxiv 802181; doi: https://doi.org/10.1101/802181
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Embryonic organoids recapitulate early heart organogenesis
Giuliana Rossi, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Matthias P. Lutolf
bioRxiv 802181; doi: https://doi.org/10.1101/802181

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