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Plants with self-sustained luminescence

Tatiana Mitiouchkina, Alexander S. Mishin, Louisa Gonzalez Somermeyer, Nadezhda M. Markina, Tatiana V. Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva, Kseniia A. Palkina, Ekaterina S. Shakhova, Liliia I. Fakhranurova, Sofia V. Chekova, Aleksandra S. Tsarkova, Yaroslav V. Golubev, Vadim V. Negrebetsky, Sergey A. Dolgushin, Pavel V. Shalaev, Olesya A. Melnik, Victoria O. Shipunova, Sergey M. Deyev, Andrey I. Bubyrev, Alexander S. Pushin, Vladimir V. Choob, Sergey V. Dolgov, Fyodor A. Kondrashov, Ilia V. Yampolsky, Karen S. Sarkisyan
doi: https://doi.org/10.1101/809376
Tatiana Mitiouchkina
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Alexander S. Mishin
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Louisa Gonzalez Somermeyer
3Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria
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Nadezhda M. Markina
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Tatiana V. Chepurnyh
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Elena B. Guglya
4Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow 117997, Russia
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Tatiana A. Karataeva
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Kseniia A. Palkina
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Ekaterina S. Shakhova
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Liliia I. Fakhranurova
1Planta LLC, 121205 Moscow, Russia
5Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Moscow, Russia
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Sofia V. Chekova
1Planta LLC, 121205 Moscow, Russia
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Aleksandra S. Tsarkova
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Yaroslav V. Golubev
4Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow 117997, Russia
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Vadim V. Negrebetsky
4Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow 117997, Russia
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Sergey A. Dolgushin
6National Research University of Electronic Technology, Shokin sq. 1, Moscow, 124498
7Gamaleya Research Center of Epidemiology and Microbiology, Gamaleya str.18, Moscow, 123098
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Pavel V. Shalaev
6National Research University of Electronic Technology, Shokin sq. 1, Moscow, 124498
7Gamaleya Research Center of Epidemiology and Microbiology, Gamaleya str.18, Moscow, 123098
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Olesya A. Melnik
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Victoria O. Shipunova
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Sergey M. Deyev
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Andrey I. Bubyrev
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Alexander S. Pushin
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Vladimir V. Choob
8Botanical Garden of Lomonosov Moscow State University, Vorobievy Gory 1 b.12, Moscow 119234 Russia
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Sergey V. Dolgov
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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Fyodor A. Kondrashov
3Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria
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Ilia V. Yampolsky
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
4Pirogov Russian National Research Medical University, Ostrovityanova 1, Moscow 117997, Russia
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  • For correspondence: ivyamp@gmail.com karen@planta.bio
Karen S. Sarkisyan
1Planta LLC, 121205 Moscow, Russia
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
98Synthetic Biology Group, MRC London Institute of Medical Sciences, London, UK
99Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK
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  • For correspondence: ivyamp@gmail.com karen@planta.bio
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Abstract

In contrast to fluorescent proteins, light emission from luciferase reporters requires exogenous addition of a luciferin substrate. Bacterial bioluminescence has been the single exception, where an operon of five genes is sufficient to produce light autonomously. Although commonly used in prokaryotic hosts, toxicity of the aldehyde substrate has limited its use in eukaryotes1. Here we demonstrate autonomous luminescence in a multicellular eukaryotic organism by incorporating a recently discovered fungal bioluminescent system2 into tobacco plants. We monitored these light-emitting plants from germination to flowering, observing temporal and spatial patterns of luminescence across time scales from seconds to months. The dynamic patterns of luminescence reflected progression through developmental stages, circadian oscillations, transport, and response to injuries. As with other fluorescent and luminescent reporters, we anticipate that this system will be further engineered for varied purposes, especially where exogenous addition of substrate is undesirable.

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Posted October 18, 2019.
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Plants with self-sustained luminescence
Tatiana Mitiouchkina, Alexander S. Mishin, Louisa Gonzalez Somermeyer, Nadezhda M. Markina, Tatiana V. Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva, Kseniia A. Palkina, Ekaterina S. Shakhova, Liliia I. Fakhranurova, Sofia V. Chekova, Aleksandra S. Tsarkova, Yaroslav V. Golubev, Vadim V. Negrebetsky, Sergey A. Dolgushin, Pavel V. Shalaev, Olesya A. Melnik, Victoria O. Shipunova, Sergey M. Deyev, Andrey I. Bubyrev, Alexander S. Pushin, Vladimir V. Choob, Sergey V. Dolgov, Fyodor A. Kondrashov, Ilia V. Yampolsky, Karen S. Sarkisyan
bioRxiv 809376; doi: https://doi.org/10.1101/809376
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Plants with self-sustained luminescence
Tatiana Mitiouchkina, Alexander S. Mishin, Louisa Gonzalez Somermeyer, Nadezhda M. Markina, Tatiana V. Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva, Kseniia A. Palkina, Ekaterina S. Shakhova, Liliia I. Fakhranurova, Sofia V. Chekova, Aleksandra S. Tsarkova, Yaroslav V. Golubev, Vadim V. Negrebetsky, Sergey A. Dolgushin, Pavel V. Shalaev, Olesya A. Melnik, Victoria O. Shipunova, Sergey M. Deyev, Andrey I. Bubyrev, Alexander S. Pushin, Vladimir V. Choob, Sergey V. Dolgov, Fyodor A. Kondrashov, Ilia V. Yampolsky, Karen S. Sarkisyan
bioRxiv 809376; doi: https://doi.org/10.1101/809376

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