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Building customizable auto-luminescent luciferase-based reporters in plants

View ORCID ProfileArjun Khakhar, View ORCID ProfileColby Starker, James Chamness, Nayoung Lee, Sydney Stokke, Cecily Wang, Ryan Swanson, Furva Rizvi, View ORCID ProfileTakato Imaizumi, View ORCID ProfileDan Voytas
doi: https://doi.org/10.1101/809533
Arjun Khakhar
1College of Biological Sciences, University of Minnesota, MN, 55108
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Colby Starker
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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James Chamness
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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Nayoung Lee
4Department of Biology, University of Washington, Seattle, WA, 98195-1800
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Sydney Stokke
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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Cecily Wang
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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Ryan Swanson
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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Furva Rizvi
3Department Biomedical engineering, University of Minnesota, MN, 55108
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Takato Imaizumi
4Department of Biology, University of Washington, Seattle, WA, 98195-1800
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Dan Voytas
2Department Genetics, Cell Biology, & Development, University of Minnesota, MN, 55108
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  • For correspondence: voytas@umn.edu
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Abstract

Bioluminescence is a powerful biological signal that scientists have repurposed to design reporters for gene expression in plants and animals. However, there are some downsides associated with the need to provide a substrate to these reporters, such as its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using an easily composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for the future construction of programmable auto-luminescent plant traits, such as creating light driven plant-pollinator interactions or light emitting plant-based sensors.

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Posted October 17, 2019.
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Building customizable auto-luminescent luciferase-based reporters in plants
Arjun Khakhar, Colby Starker, James Chamness, Nayoung Lee, Sydney Stokke, Cecily Wang, Ryan Swanson, Furva Rizvi, Takato Imaizumi, Dan Voytas
bioRxiv 809533; doi: https://doi.org/10.1101/809533
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Building customizable auto-luminescent luciferase-based reporters in plants
Arjun Khakhar, Colby Starker, James Chamness, Nayoung Lee, Sydney Stokke, Cecily Wang, Ryan Swanson, Furva Rizvi, Takato Imaizumi, Dan Voytas
bioRxiv 809533; doi: https://doi.org/10.1101/809533

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