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Individual neuronal subtypes control initial myelin sheath growth and stabilization

View ORCID ProfileHeather N. Nelson, View ORCID ProfileAnthony J. Treichel, Erin N. Eggum, Madeline R. Martell, Amanda J. Kaiser, Allie G. Trudel, James R. Gronseth, Samantha T. Maas, Silas Bergen, View ORCID ProfileJacob H. Hines
doi: https://doi.org/10.1101/809996
Heather N. Nelson
Biology Department, Winona State University, Winona, MN
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Anthony J. Treichel
Biology Department, Winona State University, Winona, MN
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Erin N. Eggum
Biology Department, Winona State University, Winona, MN
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Madeline R. Martell
Biology Department, Winona State University, Winona, MN
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Amanda J. Kaiser
Biology Department, Winona State University, Winona, MN
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Allie G. Trudel
Biology Department, Winona State University, Winona, MN
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James R. Gronseth
Biology Department, Winona State University, Winona, MN
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Samantha T. Maas
Biology Department, Winona State University, Winona, MN
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Silas Bergen
Biology Department, Winona State University, Winona, MN
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Jacob H. Hines
Biology Department, Winona State University, Winona, MN
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  • For correspondence: jhhines@winona.edu
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Abstract

Background In the developing central nervous system, pre-myelinating oligodendrocytes sample candidate nerve axons by extending and retracting process extensions. Some contacts stabilize, leading to the initiation of axon wrapping, nascent myelin sheath formation, concentric wrapping and sheath elongation, and sheath stabilization or pruning by oligodendrocytes. Although axonal signals influence the overall process of myelination, the precise oligodendrocyte behaviors that require signaling from axons are not completely understood. In this study, we investigated whether oligodendrocyte behaviors during the early events of myelination are mediated by an oligodendrocyte-intrinsic myelination program or are over-ridden by axonal factors.

Methods To address this, we utilized in vivo time-lapse imaging in embryonic and larval zebrafish spinal cord during the initial hours and days of axon wrapping and myelination. Transgenic reporter lines marked individual axon subtypes or oligodendrocyte membranes.

Results In the larval zebrafish spinal cord, individual axon subtypes supported distinct nascent sheath growth rates and stabilization frequencies. Oligodendrocytes ensheathed individual axon subtypes at different rates during a two-day period after initial axon wrapping. When descending reticulospinal axons were ablated, local spinal axons supported a constant ensheathment rate despite the increased ratio of oligodendrocytes to target axons.

Conclusion We conclude that properties of individual axon subtypes instruct oligodendrocyte behaviors during initial stages of myelination by differentially controlling nascent sheath growth and stabilization.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 18, 2019.
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Individual neuronal subtypes control initial myelin sheath growth and stabilization
Heather N. Nelson, Anthony J. Treichel, Erin N. Eggum, Madeline R. Martell, Amanda J. Kaiser, Allie G. Trudel, James R. Gronseth, Samantha T. Maas, Silas Bergen, Jacob H. Hines
bioRxiv 809996; doi: https://doi.org/10.1101/809996
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Individual neuronal subtypes control initial myelin sheath growth and stabilization
Heather N. Nelson, Anthony J. Treichel, Erin N. Eggum, Madeline R. Martell, Amanda J. Kaiser, Allie G. Trudel, James R. Gronseth, Samantha T. Maas, Silas Bergen, Jacob H. Hines
bioRxiv 809996; doi: https://doi.org/10.1101/809996

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