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A presynaptic spectrin network controls active zone assembly and neurotransmitter release

Qi Wang, Lindsey Friend, Rosario Vicidomini, Tae Hee Han, Peter Nguyen, Chun-Yuan Ting, Mihaela Serpe
doi: https://doi.org/10.1101/812032
Qi Wang
1, Bethesda, MD, 20892, USA
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Lindsey Friend
1, Bethesda, MD, 20892, USA
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Rosario Vicidomini
1, Bethesda, MD, 20892, USA
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Tae Hee Han
1, Bethesda, MD, 20892, USA
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Peter Nguyen
1, Bethesda, MD, 20892, USA
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Chun-Yuan Ting
2, Bethesda, MD, 20892, USA
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Mihaela Serpe
1, Bethesda, MD, 20892, USA
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  • For correspondence: mihaela.serpe@nih.gov
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ABSTRACT

We have previously reported that Drosophila Tenectin (Tnc) recruits αPS2/βPS integrin to ensure structural and functional integrity at larval NMJs (Wang et al., 2018). In muscles, Tnc/integrin engages the spectrin network to regulate the size and architecture of synaptic boutons. In neurons, Tnc/integrin controls neurotransmitter release. Here we show that presynaptic Tnc/integrin modulates the synaptic accumulation of key active zone components, including the Ca2+ channel Cac and the active zone scaffold Brp. Presynaptic α-Spectrin appears to be both required and sufficient for the recruitment of Cac and Brp. We visualized the endogenous α-Spectrin and found that Tnc controls spectrin recruitment at synaptic terminals. Thus, Tnc/integrin anchors the presynaptic spectrin network and ensures the proper assembly and function of the active zones. Since pre- and postsynaptic Tnc/integrin limit each other, we hypothesize that this pathway links dynamic changes within the synaptic cleft to changes in synaptic structure and function.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 21, 2019.
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A presynaptic spectrin network controls active zone assembly and neurotransmitter release
Qi Wang, Lindsey Friend, Rosario Vicidomini, Tae Hee Han, Peter Nguyen, Chun-Yuan Ting, Mihaela Serpe
bioRxiv 812032; doi: https://doi.org/10.1101/812032
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A presynaptic spectrin network controls active zone assembly and neurotransmitter release
Qi Wang, Lindsey Friend, Rosario Vicidomini, Tae Hee Han, Peter Nguyen, Chun-Yuan Ting, Mihaela Serpe
bioRxiv 812032; doi: https://doi.org/10.1101/812032

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