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Oxygenation properties of hemoglobin and the evolutionary origins of isoform multiplicity in an amphibious air-breathing fish, the blue-spotted mudskipper (Boleophthalmus pectinirostris)

View ORCID ProfileJay F. Storz, Chandrasekhar Natarajan, Magnus K. Grouleff, Michael Vandewege, View ORCID ProfileFederico G. Hoffmann, Xinxin You, Byrappa Venkatesh, Angela Fago
doi: https://doi.org/10.1101/818807
Jay F. Storz
1School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA
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  • For correspondence: jstorz2@unl.edu
Chandrasekhar Natarajan
1School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA
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Magnus K. Grouleff
2Zoophysiology, Department of Bioscience, Aarhus University, C. F. Møllers Alle 3, Aarhus C 8000, Denmark
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Michael Vandewege
3Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USA
4Institute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA
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Federico G. Hoffmann
3Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USA
4Institute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA
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Xinxin You
5Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI-Marine, BGI, Shenzhen, 518083, China
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Byrappa Venkatesh
6Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673, Singapore
7Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore
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Angela Fago
2Zoophysiology, Department of Bioscience, Aarhus University, C. F. Møllers Alle 3, Aarhus C 8000, Denmark
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ABSTRACT

Among the numerous lineages of teleost fish that have independently transitioned from obligate water-breathing to facultative air-breathing, evolved properties of hemoglobin (Hb)-O2 transport may have been shaped by the prevalence and severity of aquatic hypoxia (which influences the extent to which fish are compelled to switch to aerial respiration) as well as the anatomical design of air-breathing structures and the cardiovascular system. Here we examine the structure and function of Hbs in an amphibious, facultative air-breathing fish, the blue-spotted mudskipper (Boleophthalmus pectinirostris). We also characterized the genomic organization of the globin gene clusters of the species and we integrated phylogenetic and comparative genomic analyses to unravel the duplicative history of the genes that encode the subunits of structurally distinct mudskipper Hb isoforms (isoHbs). The B. pectinirostris isoHbs exhibit high intrinsic O2-affinities, similar to those of hypoxia-tolerant, water-breathing teleosts, and remarkably large Bohr effects. Genomic analysis of conserved synteny revealed that the genes that encode the α-type subunits of the two main adult isoHbs are members of paralogous gene clusters that represent products of the teleost-specific whole-genome duplication. Experiments revealed no appreciable difference in the oxygenation properties of co-expressed isoHbs in spite of extensive amino acid divergence between the alternative α-chain subunit isoforms. It therefore appears that the ability to switch between aquatic and aerial respiration does not necessarily require a division of labor between functionally distinct isoHbs with specialized oxygenation properties.

Summary statement The blue-spotted mudskipper routinely switches between aquatic and aerial respiration. This respiratory versatility is associated with properties of hemoglobin-oxygen transport that are similar to those found in hypoxia-adapted water-breathing fishes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 25, 2019.
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Oxygenation properties of hemoglobin and the evolutionary origins of isoform multiplicity in an amphibious air-breathing fish, the blue-spotted mudskipper (Boleophthalmus pectinirostris)
Jay F. Storz, Chandrasekhar Natarajan, Magnus K. Grouleff, Michael Vandewege, Federico G. Hoffmann, Xinxin You, Byrappa Venkatesh, Angela Fago
bioRxiv 818807; doi: https://doi.org/10.1101/818807
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Oxygenation properties of hemoglobin and the evolutionary origins of isoform multiplicity in an amphibious air-breathing fish, the blue-spotted mudskipper (Boleophthalmus pectinirostris)
Jay F. Storz, Chandrasekhar Natarajan, Magnus K. Grouleff, Michael Vandewege, Federico G. Hoffmann, Xinxin You, Byrappa Venkatesh, Angela Fago
bioRxiv 818807; doi: https://doi.org/10.1101/818807

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