ABSTRACT
Protein synthesis is dysregulated in many diseases, but we lack a systems-level picture of how signaling molecules and RNA binding proteins interact with the translational machinery, largely due to technological limitations. Here we present riboPLATE-seq, a scalable method for generating paired libraries of ribosome-associated and total mRNA. As an extension of the PLATE-seq protocol, riboPLATE-seq utilizes barcoded primers for pooled library preparation, but additionally leverages rRNA immunoprecipitation on whole polysomes to measure ribosome association (RA). We demonstrate the performance of riboPLATE-seq and its utility in detecting translational alterations induced by inhibition of protein kinases.
Footnotes
↵† Co-first authors
Contact Information: Jordan Metz – jm3423{at}cumc.columbia.edu, Nicholas Hornstein – njh219{at}gmail.com, Sohani Das Sharma – sohani.dassharma{at}gmail.com, Jeremy Worley – jw3409{at}cumc.columbia.edu
https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/geo/query/acc.cgi?acc=GSE139238