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Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module

View ORCID ProfileEugene G. Maksimov, Alexey V. Zamaraev, Evgenia Yu. Parshina, Yury B. Slonimskiy, Tatiana A. Slastnikova, Alibek A. Abdrakhmanov, Pavel A. Babaev, Svetlana S. Efimova, Olga S. Ostroumova, Alexey V. Stepanov, Anastasia V. Ryabova, Thomas Friedrich, Nikolai N. Sluchanko
doi: https://doi.org/10.1101/823880
Eugene G. Maksimov
1Lomonosov Moscow State University, Department of Biophysics, Faculty of Biology, 119991, Moscow, Russia
2A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia
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  • ORCID record for Eugene G. Maksimov
  • For correspondence: emaksimoff@yandex.ru
Alexey V. Zamaraev
3Faculty of Basic Medicine, MV Lomonosov Moscow State University, Moscow, Russia
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Evgenia Yu. Parshina
1Lomonosov Moscow State University, Department of Biophysics, Faculty of Biology, 119991, Moscow, Russia
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Yury B. Slonimskiy
2A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia
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Tatiana A. Slastnikova
5Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
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Alibek A. Abdrakhmanov
3Faculty of Basic Medicine, MV Lomonosov Moscow State University, Moscow, Russia
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Pavel A. Babaev
1Lomonosov Moscow State University, Department of Biophysics, Faculty of Biology, 119991, Moscow, Russia
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Svetlana S. Efimova
6Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave.4, St. Petersburg, 194064
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Olga S. Ostroumova
6Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave.4, St. Petersburg, 194064
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Alexey V. Stepanov
7M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia
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Anastasia V. Ryabova
8A.M.Prokhorov General Physics Institute RAS, 119991 Moscow, Russia
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Thomas Friedrich
9Technical University of Berlin, Institute of Chemistry PC 14, Straβe des 17. Juni 135, D-10623, Berlin, Germany
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Nikolai N. Sluchanko
1Lomonosov Moscow State University, Department of Biophysics, Faculty of Biology, 119991, Moscow, Russia
2A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia
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Abstract

To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena (termed CTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet-purple protein samples amenable to lyophilization and long-term storage. We characterize spectroscopic properties of the pigment-protein complexes and thermodynamics of liposome-protein carotenoid transfer and demonstrate the highly efficient delivery of echinenone form CTDH into liposomes. Most importantly, we show carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species. The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.

Significance statement Carotenoids are excellent natural antioxidants but their delivery to vulnerable cells is challenging due to their hydrophobic nature and susceptibility to degradation. Thus, systems securing antioxidant stability and facilitating targeted delivery are of great interest for the design of medical agents. In this work, we have demonstrated that soluble cyanobacterial carotenoprotein can deliver echinenone into membranes of liposomes and mammalian cells with almost 70 % efficiency, which alleviates the induced oxidative stress. Our findings warrant the robustness of the protein-based carotenoid delivery for studies of carotenoid activities and effects on cell models.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 30, 2019.
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Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module
Eugene G. Maksimov, Alexey V. Zamaraev, Evgenia Yu. Parshina, Yury B. Slonimskiy, Tatiana A. Slastnikova, Alibek A. Abdrakhmanov, Pavel A. Babaev, Svetlana S. Efimova, Olga S. Ostroumova, Alexey V. Stepanov, Anastasia V. Ryabova, Thomas Friedrich, Nikolai N. Sluchanko
bioRxiv 823880; doi: https://doi.org/10.1101/823880
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Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module
Eugene G. Maksimov, Alexey V. Zamaraev, Evgenia Yu. Parshina, Yury B. Slonimskiy, Tatiana A. Slastnikova, Alibek A. Abdrakhmanov, Pavel A. Babaev, Svetlana S. Efimova, Olga S. Ostroumova, Alexey V. Stepanov, Anastasia V. Ryabova, Thomas Friedrich, Nikolai N. Sluchanko
bioRxiv 823880; doi: https://doi.org/10.1101/823880

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