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A Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos

View ORCID ProfilePaul P. Geurink, View ORCID ProfileRaymond Kooij, View ORCID ProfileAysegul Sapmaz, Sijia Liu, Bo-Tao Xin, George M. C. Janssen, View ORCID ProfilePeter A. van Veelen, View ORCID ProfilePeter ten Dijke, View ORCID ProfileHuib Ovaa
doi: https://doi.org/10.1101/827642
Paul P. Geurink
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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  • ORCID record for Paul P. Geurink
  • For correspondence: p.p.geurink@lumc.nl h.ovaa@lumc.nl
Raymond Kooij
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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Aysegul Sapmaz
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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Sijia Liu
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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Bo-Tao Xin
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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George M. C. Janssen
bCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZC, Leiden, The Netherlands
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Peter A. van Veelen
bCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZC, Leiden, The Netherlands
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  • ORCID record for Peter A. van Veelen
Peter ten Dijke
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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Huib Ovaa
aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
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  • ORCID record for Huib Ovaa
  • For correspondence: p.p.geurink@lumc.nl h.ovaa@lumc.nl
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Article Information

doi 
https://doi.org/10.1101/827642
History 
  • November 1, 2019.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Paul P. Geurink*,a,‡,
  2. Raymond Kooija,‡,
  3. Aysegul Sapmaza,‡,
  4. Sijia Liua,‡,
  5. Bo-Tao Xina,
  6. George M. C. Janssenb,
  7. Peter A. van Veelenb,
  8. Peter ten Dijkea and
  9. Huib Ovaaa,*
  1. aOncode Institute & Department of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
  2. bCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZC, Leiden, The Netherlands
  1. ↵*Corresponding authors: p.p.geurink{at}lumc.nl, h.ovaa{at}lumc.nl
  1. ↵‡ Equal contribution

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A Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
Paul P. Geurink, Raymond Kooij, Aysegul Sapmaz, Sijia Liu, Bo-Tao Xin, George M. C. Janssen, Peter A. van Veelen, Peter ten Dijke, Huib Ovaa
bioRxiv 827642; doi: https://doi.org/10.1101/827642
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A Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
Paul P. Geurink, Raymond Kooij, Aysegul Sapmaz, Sijia Liu, Bo-Tao Xin, George M. C. Janssen, Peter A. van Veelen, Peter ten Dijke, Huib Ovaa
bioRxiv 827642; doi: https://doi.org/10.1101/827642

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