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Biofilm rupture by laser-induced stress waves increases with loading amplitude, independent of location

Kaitlyn L. Kearns, James D. Boyd, View ORCID ProfileMartha E. Grady
doi: https://doi.org/10.1101/827964
Kaitlyn L. Kearns
Department of Mechanical Engineering, University of Kentucky, 506 Administration Drive, Lexington, KY, 40506
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James D. Boyd
Department of Mechanical Engineering, University of Kentucky, 506 Administration Drive, Lexington, KY, 40506
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Martha E. Grady
Department of Mechanical Engineering, University of Kentucky, 506 Administration Drive, Lexington, KY, 40506
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  • ORCID record for Martha E. Grady
  • For correspondence: m.grady@uky.edu
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Abstract

Integral to the production of safe and biocompatible medical devices is to determine the interfacial properties that affect or control strong biofilm adhesion. The laser spallation technique has recently emerged as an advantageous method to quantify biofilm adhesion across candidate biomedical surfaces. However, there is a possibility that membrane tension is a factor that contributes to the stress required to separate biofilm and substrate. In that case, the stress amplitude, controlled by laser fluence, that initiates biofilm rupture would vary systematically with location on the biofilm. Film rupture, also known as spallation, occurs when film material is ejected during stress wave loading. In order to determine effects of membrane tension, we present a protocol that measures spall size with increasing laser fluence (variable fluence) and with respect to distance from the biofilm centroid (iso-fluence). Streptococcus mutans biofilms on titanium substrates serves as our model system. A total of 185 biofilm loading locations are analyzed in this study. We demonstrate that biofilm spall size increases monotonically with laser fluence and apply our procedure to failure of non-biological films. In iso-fluence experiments, no correlation is found between biofilm spall size and loading location, thus providing evidence that membrane tension does not play a dominant role in biofilm adhesion measurements. We recommend our procedure as a straightforward method to determine membrane effects in the measurement of adhesion of biological films on substrate surfaces via the laser spallation technique.

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  • https://doi.org/10.18126/5LY6-ORUL

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 01, 2019.
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Biofilm rupture by laser-induced stress waves increases with loading amplitude, independent of location
Kaitlyn L. Kearns, James D. Boyd, Martha E. Grady
bioRxiv 827964; doi: https://doi.org/10.1101/827964
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Biofilm rupture by laser-induced stress waves increases with loading amplitude, independent of location
Kaitlyn L. Kearns, James D. Boyd, Martha E. Grady
bioRxiv 827964; doi: https://doi.org/10.1101/827964

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