Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

View ORCID ProfileLucy I. Crouch, View ORCID ProfileMarcelo V. Liberato, View ORCID ProfilePaulina A. Urbanowicz, View ORCID ProfileArnaud Baslé, View ORCID ProfileChristopher A. Lamb, View ORCID ProfileChristopher J. Stewart, Katie Cooke, Mary Doona, Stephanie Needham, Richard R. Brady, Janet E. Berrington, Katarina Madunic, View ORCID ProfileManfred Wuhrer, View ORCID ProfilePeter Chater, View ORCID ProfileJeffery P. Pearson, Robert Glowacki, View ORCID ProfileEric C. Martens, Fuming Zhang, View ORCID ProfileRobert J. Linhardt, Daniel I. R. Spencer, View ORCID ProfileDavid N. Bolam
doi: https://doi.org/10.1101/835843
Lucy I. Crouch
1Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lucy I. Crouch
  • For correspondence: david.bolam@ncl.ac.uk lucy.crouch@ncl.ac.uk
Marcelo V. Liberato
2Universidade de Sorocaba, Programa de Processos Tecnológicos e Ambientais, Sorocaba, SP, Brasil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marcelo V. Liberato
Paulina A. Urbanowicz
3Ludger Ltd, Culham Science Centre, Abingdon, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paulina A. Urbanowicz
Arnaud Baslé
1Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Arnaud Baslé
Christopher A. Lamb
6Department of Gastroenterology, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christopher A. Lamb
Christopher J. Stewart
4Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christopher J. Stewart
Katie Cooke
4Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mary Doona
6Department of Gastroenterology, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie Needham
7Department of Histopathology, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard R. Brady
5Department of Colorectal Surgery, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Janet E. Berrington
8Newcastle Neonatal Service, Royal Victoria Infirmary, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katarina Madunic
9Centre for Proteomics and Metabolomics, Leiden University Medical Centre, Leiden, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Manfred Wuhrer
9Centre for Proteomics and Metabolomics, Leiden University Medical Centre, Leiden, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Manfred Wuhrer
Peter Chater
1Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Peter Chater
Jeffery P. Pearson
1Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeffery P. Pearson
Robert Glowacki
10Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric C. Martens
10Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Eric C. Martens
Fuming Zhang
11Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert J. Linhardt
11Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robert J. Linhardt
Daniel I. R. Spencer
3Ludger Ltd, Culham Science Centre, Abingdon, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David N. Bolam
1Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David N. Bolam
  • For correspondence: david.bolam@ncl.ac.uk lucy.crouch@ncl.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The human gut microbiota (HGM) are closely associated with health, development and disease. The thick intestinal mucus layer, especially in the colon, is the key barrier between the contents of the lumen and the epithelial cells, providing protection against infiltration by the microbiota as well potential pathogens. The upper layer of the colonic mucus is a niche for a subset of the microbiota which utilise the mucin glycoproteins as a nutrient source and mucin grazing by the microbiota appears to play a key role in maintaining barrier function as well as community stability. Despite the importance of mucin breakdown for gut health, the mechanisms by which gut bacteria access this complex glycoprotein are not well understood. The current model for mucin degradation involves exclusively exo-acting glycosidases that sequentially trim monosaccharides from the termini of the glycan chains to eventually allow access to the mucin peptide backbone by proteases. However, this model is in direct contrast to the Sus paradigm of glycan breakdown used by the Bacteroidetes which involves extracellular cleavage of glycans by surface located endo-acting enzymes prior to import of the oligosaccharide products. Here we describe the discovery and characterisation of endo-acting family 16 glycoside hydrolases (GH16s) from prominent mucin degrading gut bacteria that specifically target the oligosaccharide side chains of intestinal mucins from both animals and humans. These endo-acting O-glycanases display β1,4-glactosidase activity and in several cases are surface located indicating they are involved in the initial step in mucin breakdown. The data suggest a new paradigm for mucin breakdown by the microbiota and the endo-mucinases provide a potential tool to explore changes that occur in mucin structure in intestinal disorders such as inflammatory bowel disease and colon cancer.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted November 09, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
Lucy I. Crouch, Marcelo V. Liberato, Paulina A. Urbanowicz, Arnaud Baslé, Christopher A. Lamb, Christopher J. Stewart, Katie Cooke, Mary Doona, Stephanie Needham, Richard R. Brady, Janet E. Berrington, Katarina Madunic, Manfred Wuhrer, Peter Chater, Jeffery P. Pearson, Robert Glowacki, Eric C. Martens, Fuming Zhang, Robert J. Linhardt, Daniel I. R. Spencer, David N. Bolam
bioRxiv 835843; doi: https://doi.org/10.1101/835843
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
Lucy I. Crouch, Marcelo V. Liberato, Paulina A. Urbanowicz, Arnaud Baslé, Christopher A. Lamb, Christopher J. Stewart, Katie Cooke, Mary Doona, Stephanie Needham, Richard R. Brady, Janet E. Berrington, Katarina Madunic, Manfred Wuhrer, Peter Chater, Jeffery P. Pearson, Robert Glowacki, Eric C. Martens, Fuming Zhang, Robert J. Linhardt, Daniel I. R. Spencer, David N. Bolam
bioRxiv 835843; doi: https://doi.org/10.1101/835843

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (4655)
  • Biochemistry (10309)
  • Bioengineering (7629)
  • Bioinformatics (26211)
  • Biophysics (13455)
  • Cancer Biology (10635)
  • Cell Biology (15354)
  • Clinical Trials (138)
  • Developmental Biology (8459)
  • Ecology (12762)
  • Epidemiology (2067)
  • Evolutionary Biology (16777)
  • Genetics (11367)
  • Genomics (15413)
  • Immunology (10559)
  • Microbiology (25063)
  • Molecular Biology (10164)
  • Neuroscience (54145)
  • Paleontology (398)
  • Pathology (1657)
  • Pharmacology and Toxicology (2878)
  • Physiology (4319)
  • Plant Biology (9206)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6759)
  • Zoology (1453)