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Specificity in auxin responses is not explained by the promoter preferences of activator ARFs

Amy Lanctot, Mallorie Taylor-Teeples, Erika A. Oki, View ORCID ProfileJennifer L. Nemhauser
doi: https://doi.org/10.1101/843391
Amy Lanctot
Department of Biology, University of Washington, Seattle, Washington 98195
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Mallorie Taylor-Teeples
Department of Biology, University of Washington, Seattle, Washington 98195
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Erika A. Oki
Department of Biology, University of Washington, Seattle, Washington 98195
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Jennifer L. Nemhauser
Department of Biology, University of Washington, Seattle, Washington 98195
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  • ORCID record for Jennifer L. Nemhauser
  • For correspondence: jn7@uw.edu
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Abstract

Auxin is essential for almost every developmental process within plants. How a single small molecule can lead to a plethora of downstream responses has puzzled researchers for decades. It has been hypothesized that one source for such diversity is distinct promoter-binding and activation preferences for different members of the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. We systematically tested this hypothesis by engineering varied promoter sequences in a heterologous yeast system and quantifying transcriptional activation by ARFs from two species, Arabidopsis thaliana and Zea mays. By harnessing the user-defined and scalable nature of our synthetic system, we elucidated promoter design rules for optimal ARF function, discovered novel ARF-responsive promoters, and characterized the impact of ARF dimerization on their activation potential. We found no evidence for specificity in ARF-promoter interactions, suggesting that the diverse auxin responses observed in plants may be driven by factors outside the core auxin response machinery.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 15, 2019.
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Specificity in auxin responses is not explained by the promoter preferences of activator ARFs
Amy Lanctot, Mallorie Taylor-Teeples, Erika A. Oki, Jennifer L. Nemhauser
bioRxiv 843391; doi: https://doi.org/10.1101/843391
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Specificity in auxin responses is not explained by the promoter preferences of activator ARFs
Amy Lanctot, Mallorie Taylor-Teeples, Erika A. Oki, Jennifer L. Nemhauser
bioRxiv 843391; doi: https://doi.org/10.1101/843391

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