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Minimal genome-wide human CRISPR-Cas9 library

View ORCID ProfileEmanuel Gonçalves, Mark Thomas, Fiona M Behan, Gabriele Picco, Clare Pacini, Felicity Allen, David Parry-Smith, Francesco Iorio, Leopold Parts, Kosuke Yusa, View ORCID ProfileMathew J Garnett
doi: https://doi.org/10.1101/848895
Emanuel Gonçalves
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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  • ORCID record for Emanuel Gonçalves
Mark Thomas
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Fiona M Behan
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Gabriele Picco
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Clare Pacini
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Felicity Allen
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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David Parry-Smith
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Francesco Iorio
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Leopold Parts
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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Kosuke Yusa
Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
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Mathew J Garnett
Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK
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  • ORCID record for Mathew J Garnett
  • For correspondence: mg12@sanger.ac.uk
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Abstract

CRISPR guide-RNA libraries have been iteratively optimised to provide increasingly efficient reagents although their large size is a barrier for some applications. We designed a minimal genome-wide human CRISPR-Cas9 library (MinLibCas9), optimised by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 46% reduction in size compared to other libraries while preserving assay sensitivity and specificity. MinLibCas9 improves the efficiency of CRISPR-Cas9 loss-of-function screens and expands their application to complex models and assays.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 21, 2019.
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Minimal genome-wide human CRISPR-Cas9 library
Emanuel Gonçalves, Mark Thomas, Fiona M Behan, Gabriele Picco, Clare Pacini, Felicity Allen, David Parry-Smith, Francesco Iorio, Leopold Parts, Kosuke Yusa, Mathew J Garnett
bioRxiv 848895; doi: https://doi.org/10.1101/848895
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Minimal genome-wide human CRISPR-Cas9 library
Emanuel Gonçalves, Mark Thomas, Fiona M Behan, Gabriele Picco, Clare Pacini, Felicity Allen, David Parry-Smith, Francesco Iorio, Leopold Parts, Kosuke Yusa, Mathew J Garnett
bioRxiv 848895; doi: https://doi.org/10.1101/848895

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