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Multiplexed biosensors for precision bacteria tropism in vivo

View ORCID ProfileTiffany Chien, View ORCID ProfileTetsuhiro Harimoto, Benjamin Kepecs, Kelsey Gray, Courtney Coker, Kelly Pu, Tamjeed Azad, View ORCID ProfileTal Danino
doi: https://doi.org/10.1101/851311
Tiffany Chien
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Tetsuhiro Harimoto
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Benjamin Kepecs
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Kelsey Gray
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Courtney Coker
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Kelly Pu
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Tamjeed Azad
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
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Tal Danino
1Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
2Data Science Institute, Columbia University, New York, NY 10027, USA
3Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY 10027, USA
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  • For correspondence: td2506@columbia.edu
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Abstract

The engineering of microbes spurs biotechnological innovations, but requires control mechanisms to confine growth within defined environments for translation. Here we engineer bacterial growth tropism to sense and grow in response to specified oxygen, pH, and lactate signatures. Coupling biosensors to drive essential gene expression reveals engineered bacterial localization within upper or lower gastrointestinal tract. Multiplexing biosensors in an AND logic-gate architecture reduced bacterial off-target colonization in vivo.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 21, 2019.
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Multiplexed biosensors for precision bacteria tropism in vivo
Tiffany Chien, Tetsuhiro Harimoto, Benjamin Kepecs, Kelsey Gray, Courtney Coker, Kelly Pu, Tamjeed Azad, Tal Danino
bioRxiv 851311; doi: https://doi.org/10.1101/851311
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Multiplexed biosensors for precision bacteria tropism in vivo
Tiffany Chien, Tetsuhiro Harimoto, Benjamin Kepecs, Kelsey Gray, Courtney Coker, Kelly Pu, Tamjeed Azad, Tal Danino
bioRxiv 851311; doi: https://doi.org/10.1101/851311

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