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Single-cell immuno-mechanics: rapid viscoelastic changes are a hall-mark of early leukocyte activation

Alexandra Zak, Sara Violeta Merino Cortés, Anaïs Sadoun, View ORCID ProfileAvin Babataheri, View ORCID ProfileStéphanie Dogniaux, View ORCID ProfileSophie Dupré-Crochet, Elodie Hudik, Hai-Tao He, View ORCID ProfileAbdul I Barakat, Yolanda R Carrasco, View ORCID ProfileYannick Hamon, View ORCID ProfilePierre-Henri Puech, View ORCID ProfileClaire Hivroz, View ORCID ProfileOliver Nüsse, View ORCID ProfileJulien Husson
doi: https://doi.org/10.1101/851634
Alexandra Zak
1Laboratoire d’Hydrodynamique (LadHyX), Ecole polytechnique, CNRS UMR7646, Institut Polytechnique de Paris, route de Saclay, 91128 Palaiseau Cedex, France
2Laboratoire de Chimie Physique, CNRS, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay, France
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Sara Violeta Merino Cortés
3B cell Dynamics Laboratory, Department on Immunology and Oncology, Centro Nacional de Biotecnología (CNB)- CSIC, Madrid, Spain
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Anaïs Sadoun
4Aix Marseille University, LAI UM 61, Marseille, F-13288, France
5Inserm, UMR_S 1067, Marseille, F-13288, France
6CNRS, UMR 7333, Marseille, F-13288, France
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Avin Babataheri
1Laboratoire d’Hydrodynamique (LadHyX), Ecole polytechnique, CNRS UMR7646, Institut Polytechnique de Paris, route de Saclay, 91128 Palaiseau Cedex, France
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  • ORCID record for Avin Babataheri
Stéphanie Dogniaux
7Institut Curie, PSL Research University, INSERM U932, Integrative analysis of T cell activation team, 26 rue d’Ulm, 75248, Paris Cedex 05, France
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  • ORCID record for Stéphanie Dogniaux
Sophie Dupré-Crochet
2Laboratoire de Chimie Physique, CNRS, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay, France
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Elodie Hudik
2Laboratoire de Chimie Physique, CNRS, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay, France
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Hai-Tao He
8Aix Marseille University, CNRS, INSERM, CIML, Marseille, France
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Abdul I Barakat
1Laboratoire d’Hydrodynamique (LadHyX), Ecole polytechnique, CNRS UMR7646, Institut Polytechnique de Paris, route de Saclay, 91128 Palaiseau Cedex, France
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Yolanda R Carrasco
3B cell Dynamics Laboratory, Department on Immunology and Oncology, Centro Nacional de Biotecnología (CNB)- CSIC, Madrid, Spain
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Yannick Hamon
8Aix Marseille University, CNRS, INSERM, CIML, Marseille, France
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Pierre-Henri Puech
4Aix Marseille University, LAI UM 61, Marseille, F-13288, France
5Inserm, UMR_S 1067, Marseille, F-13288, France
6CNRS, UMR 7333, Marseille, F-13288, France
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Claire Hivroz
7Institut Curie, PSL Research University, INSERM U932, Integrative analysis of T cell activation team, 26 rue d’Ulm, 75248, Paris Cedex 05, France
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Oliver Nüsse
2Laboratoire de Chimie Physique, CNRS, Université Paris-Sud, Université Paris-Saclay, 91405, Orsay, France
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Julien Husson
1Laboratoire d’Hydrodynamique (LadHyX), Ecole polytechnique, CNRS UMR7646, Institut Polytechnique de Paris, route de Saclay, 91128 Palaiseau Cedex, France
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  • For correspondence: julien.husson@ladhyx.polytechnique.fr
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Abstract

To accomplish their critical task of removing infected cells and fighting pathogens, leukocytes activate by forming specialized interfaces with other cells. Using an innovative micropipette rheometer, we show in three different cell types that when stimulated by microbeads mimicking target cells, leukocytes become up to ten times stiffer and more viscous. These mechanical changes initiate within seconds after contact and evolve rapidly over minutes. Remarkably, leukocyte elastic and viscous properties evolve in parallel, preserving a well-defined ratio that constitutes a mechanical signature specific to each cell type. The current results indicate that simultaneously tracking both elastic and viscous properties during an active cell process provides a new way to investigate cell mechanical processes. Our findings also suggest that dynamic immuno-mechanical measurements provide an identifier of leukocyte type and an indicator of the cell’s state of activation.

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Posted November 22, 2019.
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Single-cell immuno-mechanics: rapid viscoelastic changes are a hall-mark of early leukocyte activation
Alexandra Zak, Sara Violeta Merino Cortés, Anaïs Sadoun, Avin Babataheri, Stéphanie Dogniaux, Sophie Dupré-Crochet, Elodie Hudik, Hai-Tao He, Abdul I Barakat, Yolanda R Carrasco, Yannick Hamon, Pierre-Henri Puech, Claire Hivroz, Oliver Nüsse, Julien Husson
bioRxiv 851634; doi: https://doi.org/10.1101/851634
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Single-cell immuno-mechanics: rapid viscoelastic changes are a hall-mark of early leukocyte activation
Alexandra Zak, Sara Violeta Merino Cortés, Anaïs Sadoun, Avin Babataheri, Stéphanie Dogniaux, Sophie Dupré-Crochet, Elodie Hudik, Hai-Tao He, Abdul I Barakat, Yolanda R Carrasco, Yannick Hamon, Pierre-Henri Puech, Claire Hivroz, Oliver Nüsse, Julien Husson
bioRxiv 851634; doi: https://doi.org/10.1101/851634

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