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Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

View ORCID ProfileJames P. Tauber, Ralf Einspanier, Jay D. Evans, Dino P. McMahon
doi: https://doi.org/10.1101/852699
James P. Tauber
1Bee Research Laboratory, Beltsville Agricultural Research Center, US Department of Agriculture, Beltsville, MD 20705, USA
2Department for Materials and the Environment, BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, 12205, Berlin, Germany
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  • For correspondence: jamesptauber@gmail.com james.tauber@usda.gov
Ralf Einspanier
3Institute of Veterinary Biochemistry, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany
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Jay D. Evans
1Bee Research Laboratory, Beltsville Agricultural Research Center, US Department of Agriculture, Beltsville, MD 20705, USA
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Dino P. McMahon
2Department for Materials and the Environment, BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, 12205, Berlin, Germany
4Institut für Biologie, Freie Universität Berlin, Königin-Luise-Str. 1-3, 14195, Berlin, Germany
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Abstract

There are viral, fungal, bacterial and trypanosomal pathogens that negatively impact the individual and superorganismal health of the western honey bee. One fungal pathogen, Ascosphaera apis, affects larvae and causes the disease chalkbrood. A previous genome analysis of As. apis revealed that its genome encodes for RNA interference genes, similar to other fungi and eukaryotes. Here, we examined whether As. apis-targeting double-stranded RNA species could disrupt the germination of As. apis. We observed that when spores were co-incubated with As. apis-targeting dsRNA, fewer spores were activated for germination, suggesting an uptake of exogenous genetic material at the very onset of germination and consequent damage to essential transcripts needed for germination. Overall, these results indicate that the causative agent of chalkbrood disease, As. apis, can be successfully targeted using an RNAi-based strategy.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This article is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license.
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Posted November 25, 2019.
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Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen
James P. Tauber, Ralf Einspanier, Jay D. Evans, Dino P. McMahon
bioRxiv 852699; doi: https://doi.org/10.1101/852699
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Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen
James P. Tauber, Ralf Einspanier, Jay D. Evans, Dino P. McMahon
bioRxiv 852699; doi: https://doi.org/10.1101/852699

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