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Topology-driven analysis of protein-protein interaction networks detects functional genetic modules regulating reproductive capacity

Tarun Kumar, View ORCID ProfileLeo Blondel, View ORCID ProfileCassandra G. Extavour
doi: https://doi.org/10.1101/852897
Tarun Kumar
1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge MA-02138, USA
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Leo Blondel
2Department of Molecular and Cellular Biology, Harvard University, Cambridge MA-02138, USA
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Cassandra G. Extavour
1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge MA-02138, USA
2Department of Molecular and Cellular Biology, Harvard University, Cambridge MA-02138, USA
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  • For correspondence: extavour@oeb.harvard.edu
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Abstract

Understanding the genetic regulation of organ structure is a fundamental problem in developmental biology. Here, we use egg-producing structures of insect ovaries, called ovarioles, to deduce systems-level gene regulatory relationships from quantitative functional genetic analysis. We previously showed that Hippo signalling, a conserved regulator of animal organ size, regulates ovariole number in Drosophila melanogaster. To comprehensively determine how Hippo signalling interacts with other pathways in this regulation, we screened all known signalling pathway genes, and identified Hpo-dependent and Hpo-independent signalling requirements. Network analysis of known protein-protein interactions among screen results identified independent gene regulatory modules regulating one or both of ovariole number and egg laying. These modules predict involvement of previously uncharacterised genes with higher accuracy than the original candidate screen. This shows that network analysis combining functional genetic and large-scale interaction data can predict function of novel genes regulating development.

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Posted November 30, 2019.
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Topology-driven analysis of protein-protein interaction networks detects functional genetic modules regulating reproductive capacity
Tarun Kumar, Leo Blondel, Cassandra G. Extavour
bioRxiv 852897; doi: https://doi.org/10.1101/852897
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Topology-driven analysis of protein-protein interaction networks detects functional genetic modules regulating reproductive capacity
Tarun Kumar, Leo Blondel, Cassandra G. Extavour
bioRxiv 852897; doi: https://doi.org/10.1101/852897

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