Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Molecular Basis of Specific DNA Binding by the BRG1 AT-hook and Bromodomain

Julio C. Sanchez, Liyang Zhang, Stefania Evoli, Nicholas J. Schnicker, Maria Nunez-Hernandez, Liping Yu, View ORCID ProfileJeff Wereszczynski, Miles A. Pufall, Catherine A. Musselman
doi: https://doi.org/10.1101/854000
Julio C. Sanchez
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liyang Zhang
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242
2Integrated DNA Technologies IDT, Coralville, Iowa, 52241
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefania Evoli
3Department of Physics, Illinois Institute of Technology, Chicago Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicholas J. Schnicker
4Protein & Crystallography Facility, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Nunez-Hernandez
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liping Yu
5Department of Biochemistry, Carver College of Medicine NMR Core Facility, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeff Wereszczynski
3Department of Physics, Illinois Institute of Technology, Chicago Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jeff Wereszczynski
  • For correspondence: catherine.musselman@cuanschutz.edu
Miles A. Pufall
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: catherine.musselman@cuanschutz.edu
Catherine A. Musselman
1Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242
6Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: catherine.musselman@cuanschutz.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The ATP-dependent BAF chromatin remodeling complex plays a critical role in gene regulation by modulating chromatin architecture, and is frequently mutated in cancer. Indeed, subunits of the BAF complex are found to be mutated in >20% of human tumors. The mechanism by which BAF properly navigates chromatin is not fully understood, but is thought to involve a multivalent network of histone and DNA contacts. We previously identified a composite domain in the BRG1 ATPase subunit that is capable of associating with both histones and DNA in a multivalent manner. Mapping the DNA binding pocket revealed that it contains several cancer mutations. Here, we utilize SELEX-seq to identify the DNA specificity of this composite domain and NMR spectroscopy and molecular modelling to determine the structural basis of DNA binding. Finally, we demonstrate that cancer mutations in this domain alter the mode of DNA association.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 25, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Molecular Basis of Specific DNA Binding by the BRG1 AT-hook and Bromodomain
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Molecular Basis of Specific DNA Binding by the BRG1 AT-hook and Bromodomain
Julio C. Sanchez, Liyang Zhang, Stefania Evoli, Nicholas J. Schnicker, Maria Nunez-Hernandez, Liping Yu, Jeff Wereszczynski, Miles A. Pufall, Catherine A. Musselman
bioRxiv 854000; doi: https://doi.org/10.1101/854000
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
The Molecular Basis of Specific DNA Binding by the BRG1 AT-hook and Bromodomain
Julio C. Sanchez, Liyang Zhang, Stefania Evoli, Nicholas J. Schnicker, Maria Nunez-Hernandez, Liping Yu, Jeff Wereszczynski, Miles A. Pufall, Catherine A. Musselman
bioRxiv 854000; doi: https://doi.org/10.1101/854000

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (2536)
  • Biochemistry (4983)
  • Bioengineering (3487)
  • Bioinformatics (15242)
  • Biophysics (6914)
  • Cancer Biology (5404)
  • Cell Biology (7756)
  • Clinical Trials (138)
  • Developmental Biology (4543)
  • Ecology (7162)
  • Epidemiology (2059)
  • Evolutionary Biology (10240)
  • Genetics (7522)
  • Genomics (9802)
  • Immunology (4869)
  • Microbiology (13250)
  • Molecular Biology (5151)
  • Neuroscience (29496)
  • Paleontology (203)
  • Pathology (838)
  • Pharmacology and Toxicology (1468)
  • Physiology (2143)
  • Plant Biology (4759)
  • Scientific Communication and Education (1013)
  • Synthetic Biology (1339)
  • Systems Biology (4015)
  • Zoology (770)