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Towards the molecular architecture of the peroxisomal receptor docking complex

Pascal Lill, Tobias Hansen, Daniel Wendscheck, View ORCID ProfileBjoern Udo Klink, Tomasz Jeziorek, Jonas Miehling, View ORCID ProfileJulian Bender, View ORCID ProfileFriedel Drepper, Wolfgang Girzalsky, View ORCID ProfileBettina Warscheid, Ralf Erdmann, View ORCID ProfileChristos Gatsogiannis
doi: https://doi.org/10.1101/854497
Pascal Lill
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany
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Tobias Hansen
2Institute of Biochemistry and Pathobiochemistry, Faculty of Medicine, System Biochemistry, Ruhr-University Bochum, Bochum, Germany
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Daniel Wendscheck
3Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
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Bjoern Udo Klink
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany
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  • ORCID record for Bjoern Udo Klink
Tomasz Jeziorek
2Institute of Biochemistry and Pathobiochemistry, Faculty of Medicine, System Biochemistry, Ruhr-University Bochum, Bochum, Germany
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Jonas Miehling
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany
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Julian Bender
3Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
5Interdisciplinary Research Center HALOmem, Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany
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Friedel Drepper
3Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
4Signalling Research Centres BIOSS and CIBSS, University of Freiburg
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Wolfgang Girzalsky
2Institute of Biochemistry and Pathobiochemistry, Faculty of Medicine, System Biochemistry, Ruhr-University Bochum, Bochum, Germany
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Bettina Warscheid
3Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
4Signalling Research Centres BIOSS and CIBSS, University of Freiburg
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  • ORCID record for Bettina Warscheid
Ralf Erdmann
2Institute of Biochemistry and Pathobiochemistry, Faculty of Medicine, System Biochemistry, Ruhr-University Bochum, Bochum, Germany
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Christos Gatsogiannis
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany
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  • ORCID record for Christos Gatsogiannis
  • For correspondence: christos.gatsogiannis@mpi-dortmund.mpg.de
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Abstract

Import of yeast peroxisomal matrix proteins is initiated by cytosolic receptors, which specifically recognize and bind the respective cargo proteins. At the peroxisomal membrane, the cargo-loaded receptor interacts with the docking protein Pex14p that is tightly associated with Pex17p. Previous data suggest that this interaction triggers the formation of an import pore for further translocation of the cargo. The mechanistic principles are however unclear, mainly because structures of higher order assemblies are still lacking. Here, using an integrative approach, we provide the first structural characterization of the major components of the peroxisomal docking complex Pex14p/Pex17p, in a native bilayer environment and reveal its subunit organization. Our data show that three copies of Pex14p and a single copy of Pex17p assemble to form a 20 nm rod-like particle. The different subunits are arranged in a parallel manner, showing interactions along their complete sequences and providing receptor binding-sites on both membrane sides. The long rod facing the cytosol is mainly formed by the predicted coiled-coil domains of Pex14p and Pex17p, possibly providing the necessary structural support for the formation of the import pore. Further implications of Pex14p/Pex17p for formation of the peroxisomal translocon are discussed.

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Posted March 19, 2020.
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Towards the molecular architecture of the peroxisomal receptor docking complex
Pascal Lill, Tobias Hansen, Daniel Wendscheck, Bjoern Udo Klink, Tomasz Jeziorek, Jonas Miehling, Julian Bender, Friedel Drepper, Wolfgang Girzalsky, Bettina Warscheid, Ralf Erdmann, Christos Gatsogiannis
bioRxiv 854497; doi: https://doi.org/10.1101/854497
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Towards the molecular architecture of the peroxisomal receptor docking complex
Pascal Lill, Tobias Hansen, Daniel Wendscheck, Bjoern Udo Klink, Tomasz Jeziorek, Jonas Miehling, Julian Bender, Friedel Drepper, Wolfgang Girzalsky, Bettina Warscheid, Ralf Erdmann, Christos Gatsogiannis
bioRxiv 854497; doi: https://doi.org/10.1101/854497

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