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Insulin signal transduction is impaired in the type 2 diabetic retina

Youde Jiang, Li Liu, Hainan Li, Jie-Mei Wang, View ORCID ProfileJena J. Steinle
doi: https://doi.org/10.1101/856245
Youde Jiang
1Department of Ophthalmology, Visual, and Anatomical Sciences, Wayne State University, Detroit, MI USA
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Li Liu
1Department of Ophthalmology, Visual, and Anatomical Sciences, Wayne State University, Detroit, MI USA
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Hainan Li
2Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI USA
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Jie-Mei Wang
2Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI USA
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Jena J. Steinle
1Department of Ophthalmology, Visual, and Anatomical Sciences, Wayne State University, Detroit, MI USA
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  • ORCID record for Jena J. Steinle
  • For correspondence: jsteinle@med.wayne.edu
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Abstract

Rates of type 2 diabetes are reaching epidemic levels. Yet, the tissue specific alterations due to insulin resistance are only recently being investigated. The goal of the present study was to evaluate retinal insulin signal transduction in a common mouse model of type 2 diabetes, the db/db mouse. Retinal lysates from five month old male db/db and db/+ (control) mice were collected and processed for Western blotting or ELISA analyses for insulin receptor, insulin receptor substrate-1 (IRS-1), Akt, tumor necrosis factor alpha (TNFα) and caspase 3 levels. Data demonstrate increased TNFα and IRS-1 phosphorylation on serine 307. This led to decreased Akt phosphorylation on serine 473 and increased cleavage of caspase 3. Taken together, the data suggest dysfunctional insulin signaling in the retina of the db/db mouse.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted November 26, 2019.
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Insulin signal transduction is impaired in the type 2 diabetic retina
Youde Jiang, Li Liu, Hainan Li, Jie-Mei Wang, Jena J. Steinle
bioRxiv 856245; doi: https://doi.org/10.1101/856245
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Insulin signal transduction is impaired in the type 2 diabetic retina
Youde Jiang, Li Liu, Hainan Li, Jie-Mei Wang, Jena J. Steinle
bioRxiv 856245; doi: https://doi.org/10.1101/856245

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