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Chronic cortisol exposure in early development leads to neuroendocrine dysregulation in adulthood

Ellen I. Hartig, Shusen Zhu, Benjamin L. King, James A. Coffman
doi: https://doi.org/10.1101/856633
Ellen I. Hartig
1MDI Biological Laboratory, Salisbury Cove, Maine, USA
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Shusen Zhu
1MDI Biological Laboratory, Salisbury Cove, Maine, USA
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Benjamin L. King
1MDI Biological Laboratory, Salisbury Cove, Maine, USA
2University of Maine Graduate School of Biomedical Sciences and Engineering, Orono, Maine, USA
3University of Maine Department of Molecular and Biomedical Sciences, Orono, Maine, USA
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James A. Coffman
1MDI Biological Laboratory, Salisbury Cove, Maine, USA
2University of Maine Graduate School of Biomedical Sciences and Engineering, Orono, Maine, USA
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  • For correspondence: jcoffman@mdibl.org
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Abstract

Objective Chronic early life stress can affect development of the neuroendocrine stress system, leading to its persistent dysregulation and consequently increased disease risk in adulthood. One contributing factor is thought to be epigenetic programming in response to chronic cortisol exposure during early development. We have previously shown that zebrafish embryos treated chronically with cortisol develop into adults with constitutively elevated whole body cortisol and aberrant immune gene expression. The objective of the experiments reported here was to further characterize the phenotype of those adults.

Results We find that adult zebrafish derived from cortisol-treated embryos have aberrant cortisol levels, tissue distribution, and dynamics, which correlate with differential activity of key glucocorticoid-responsive regulatory genes klf9 and fkbp5 in blood and brain.

  • List of Abbreviations

    ELS
    Early life stress
    GC
    Glucocorticoid
    GR
    Glucocorticoid receptor
    ATAC-seq
    Assay for transposase accessible chromatin - sequencing
    qRT-PCR
    Quantitative reverse transcription and polymerase chain reaction
    ELISA
    Enzyme linked immunosorbant assay
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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    Posted November 26, 2019.
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    Chronic cortisol exposure in early development leads to neuroendocrine dysregulation in adulthood
    Ellen I. Hartig, Shusen Zhu, Benjamin L. King, James A. Coffman
    bioRxiv 856633; doi: https://doi.org/10.1101/856633
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    Chronic cortisol exposure in early development leads to neuroendocrine dysregulation in adulthood
    Ellen I. Hartig, Shusen Zhu, Benjamin L. King, James A. Coffman
    bioRxiv 856633; doi: https://doi.org/10.1101/856633

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