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Sterols as dietary markers for Drosophila melanogaster

Oskar Knittelfelder, Elodie Prince, Susanne Sales, Eric Fritzsche, Thomas Wöhner, Marko Brankatschk, Andrej Shevchenko
doi: https://doi.org/10.1101/857664
Oskar Knittelfelder
MPI of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
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Elodie Prince
Biotechnologisches Zentrum, Technische Universität Dresden, Tatzberg 47/49, 01309 Dresden, Germany
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Susanne Sales
MPI of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, GermanyThermo Fischer Scientific GmbH, 63303 Dreieich, Germany
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Eric Fritzsche
Julius Kühn Institut, Pillnitzer Platz 3a, 01326 Dresden, Germany
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Thomas Wöhner
Julius Kühn Institut, Pillnitzer Platz 3a, 01326 Dresden, Germany
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Marko Brankatschk
Biotechnologisches Zentrum, Technische Universität Dresden, Tatzberg 47/49, 01309 Dresden, Germany
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Andrej Shevchenko
MPI of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
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  • For correspondence: shevchenko@mpi-cbg.de
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Abstract

During cold acclimation fruit flies switch their feeding from yeast to plant food, however there are no robust markers to monitor it in the wild. Drosophila melanogaster is a sterol auxotroph and relies on dietary sterols to produce lipid membranes, lipoproteins and molting hormones. We employed shotgun lipidomics to quantify eight major food sterols in total extracts of heads, female and male genital tracts of adult flies. We found that their sterol composition is dynamic and reflective of flies diet in an organ-specific manner. Season-dependent changes observed in the organs of wild-living flies suggested that the molar ratio between yeast (ergosterol, zymosterol) and plant (sitosterol, stigmasterol) sterols is a quantifiable, generic and unequivocal marker of their feeding behavior, including cold acclimation. It provides technically simpler and more contrast readout compared to the full lipidome analysis and is suitable for ecological and environmental population-based studies.

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Posted November 29, 2019.
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Sterols as dietary markers for Drosophila melanogaster
Oskar Knittelfelder, Elodie Prince, Susanne Sales, Eric Fritzsche, Thomas Wöhner, Marko Brankatschk, Andrej Shevchenko
bioRxiv 857664; doi: https://doi.org/10.1101/857664
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Sterols as dietary markers for Drosophila melanogaster
Oskar Knittelfelder, Elodie Prince, Susanne Sales, Eric Fritzsche, Thomas Wöhner, Marko Brankatschk, Andrej Shevchenko
bioRxiv 857664; doi: https://doi.org/10.1101/857664

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