Abstract
We describe a method for label-free molecular imaging of biological materials, preserved in a native state, by using an OrbiSIMS instrument equipped with cryogenic sample handling and developing a high-pressure freezing protocol compatible with mass spectrometry. We studied the 3D distribution of quorum sensing signalling molecules, nucleobases and bacterial membrane molecules, in a mature Pseudomonas aeruginosa biofilm, with high spatial-resolution and high mass-resolution.
Footnotes
↵† Senior author at institute
Copyright
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Posted November 30, 2019.
Cryo-OrbiSIMS for 3D molecular imaging of a bacterial biofilm in its native state
