Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

View ORCID ProfileGen Urabe, Masaharu Shimada, Takumi Ogata, View ORCID ProfileSunao Katsuki
doi: https://doi.org/10.1101/860841
Gen Urabe
1Room 306, Kurokami South W2, Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1 Chu-o-ku, Kumamoto City, Kumamoto, 860-8555, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gen Urabe
  • For correspondence: g.urabe@st.cs.kumamoto-u.ac.jp
Masaharu Shimada
1Room 306, Kurokami South W2, Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1 Chu-o-ku, Kumamoto City, Kumamoto, 860-8555, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takumi Ogata
1Room 306, Kurokami South W2, Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1 Chu-o-ku, Kumamoto City, Kumamoto, 860-8555, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sunao Katsuki
2Room 306, Kurokami South W2, Institute of Pulsed Power Science, Kumamoto University, Kurokami 2-39-1 Chu-o-ku, Kumamoto City, Kumamoto 860-8555, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sunao Katsuki
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Liposomes are widely assumed to present a straightforward physical model of cells. However, almost all previous liposome experiments with pulsed electric fields (PEFs) have been conducted in low-conductivity liquids, a condition that differs significantly from that of cells in medium. Here, we prepared liposomes consisting of soy bean lecithin and cholesterol, at a molar ratio of 1:1, in higher-conductivity liquid that approximated the conditions of red blood cells in phosphate-buffered saline, with inner and outer liquid conductivities of 0.6 and 1.6 S/m, respectively. We found that a single 1.1 kV/cm, 400 μs PEF promoted cell-like spontaneous division of liposomes.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted December 01, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field
Gen Urabe, Masaharu Shimada, Takumi Ogata, Sunao Katsuki
bioRxiv 860841; doi: https://doi.org/10.1101/860841
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field
Gen Urabe, Masaharu Shimada, Takumi Ogata, Sunao Katsuki
bioRxiv 860841; doi: https://doi.org/10.1101/860841

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (2633)
  • Biochemistry (5220)
  • Bioengineering (3643)
  • Bioinformatics (15707)
  • Biophysics (7210)
  • Cancer Biology (5590)
  • Cell Biology (8039)
  • Clinical Trials (138)
  • Developmental Biology (4731)
  • Ecology (7458)
  • Epidemiology (2059)
  • Evolutionary Biology (10518)
  • Genetics (7695)
  • Genomics (10079)
  • Immunology (5144)
  • Microbiology (13819)
  • Molecular Biology (5350)
  • Neuroscience (30571)
  • Paleontology (211)
  • Pathology (870)
  • Pharmacology and Toxicology (1519)
  • Physiology (2233)
  • Plant Biology (4980)
  • Scientific Communication and Education (1036)
  • Synthetic Biology (1379)
  • Systems Biology (4129)
  • Zoology (802)