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Cultivation of Exoelectrogenic Bacteria in Conductive DNA Nanocomposite Hydrogels Yields a Programmable Biohybrid Materials System

View ORCID ProfileYong Hu, David Rehnlund, Edina Klein, Johannes Gescher, View ORCID ProfileChristof M. Niemeyer
doi: https://doi.org/10.1101/864967
Yong Hu
aKarlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany
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  • ORCID record for Yong Hu
David Rehnlund
bKarlsruhe Institute of Technology (KIT), Institute for Applied Biosciences (IAB), Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany
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Edina Klein
bKarlsruhe Institute of Technology (KIT), Institute for Applied Biosciences (IAB), Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany
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Johannes Gescher
aKarlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany
bKarlsruhe Institute of Technology (KIT), Institute for Applied Biosciences (IAB), Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany
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Christof M. Niemeyer
aKarlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany
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  • For correspondence: niemeyer@kit.edu
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Abstract

The use of living microorganisms integrated within electrochemical devices is an expanding field of research, with applications in microbial fuel cells, microbial biosensors or bioreactors. We describe the use of porous nanocomposite materials prepared by DNA polymerization of carbon nanotubes (CNT) and silica nanoparticles (SiNP) for the construction of a programmable biohybrid system containing the exoelectrogenic bacterium Shewanella oneidensis. We initially demonstrate the electrical conductivity of the CNT-containing DNA composite by employment of chronopotentiometry, electrochemical impedance spectroscopy, and cyclic voltammetry. Cultivation of Shewanella oneidensis in these materials shows that the exoelectrogenic bacteria populate the matrix of the composite, while non-exoelectrogenic Escherichia coli remain on its surface. Moreover, the ability to use extracellular electron transfer pathways is positively correlated with number of cells within the conductive synthetic biofilm matrix. The Shewanella containing composite remains stable for several days. Programmability of this biohybrid material system is demonstrated by on-demand release and degradation induced by a short-term enzymatic stimulus. The perspectives of this approach for technical applications are being discussed.

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Posted December 05, 2019.
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Cultivation of Exoelectrogenic Bacteria in Conductive DNA Nanocomposite Hydrogels Yields a Programmable Biohybrid Materials System
Yong Hu, David Rehnlund, Edina Klein, Johannes Gescher, Christof M. Niemeyer
bioRxiv 864967; doi: https://doi.org/10.1101/864967
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Cultivation of Exoelectrogenic Bacteria in Conductive DNA Nanocomposite Hydrogels Yields a Programmable Biohybrid Materials System
Yong Hu, David Rehnlund, Edina Klein, Johannes Gescher, Christof M. Niemeyer
bioRxiv 864967; doi: https://doi.org/10.1101/864967

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