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The nucleus measures shape deformation for cellular proprioception and regulates adaptive morphodynamics

Valeria Venturini, Fabio Pezzano, Frederic Català Castro, Hanna-Maria Häkkinen, Senda Jiménez-Delgado, Mariona Colomer-Rosell, Mónica Marro Sánchez, Queralt Tolosa-Ramon, Sonia Paz-López, Miguel A. Valverde, Pablo Loza-Alvarez, Michael Krieg, View ORCID ProfileStefan Wieser, View ORCID ProfileVerena Ruprecht
doi: https://doi.org/10.1101/865949
Valeria Venturini
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
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Fabio Pezzano
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
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Frederic Català Castro
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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Hanna-Maria Häkkinen
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
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Senda Jiménez-Delgado
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
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Mariona Colomer-Rosell
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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Mónica Marro Sánchez
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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Queralt Tolosa-Ramon
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
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Sonia Paz-López
3Laboratory of Molecular Physiology, Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF) Barcelona, Spain
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Miguel A. Valverde
3Laboratory of Molecular Physiology, Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF) Barcelona, Spain
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Pablo Loza-Alvarez
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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Michael Krieg
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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Stefan Wieser
1ICFO – Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Spain
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  • ORCID record for Stefan Wieser
  • For correspondence: stefan.wieser@icfo.eu verena.ruprecht@crg.eu
Verena Ruprecht
2Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain
4Universitat Pompeu Fabra (UPF), Barcelona, Spain
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  • ORCID record for Verena Ruprecht
  • For correspondence: stefan.wieser@icfo.eu verena.ruprecht@crg.eu
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Abstract

The physical microenvironment regulates cell behavior during tissue development and homeostasis. How single cells decode information about their geometrical shape under mechanical stress and physical space constraints within their local environment remains largely unknown. Here we show that the nucleus, the biggest cellular organelle, functions as a non-dissipative cellular shape deformation gauge that enables cells to continuously measure shape variations on the time scale of seconds. Inner nuclear membrane unfolding together with the relative spatial intracellular positioning of the nucleus provides physical information on the amplitude and type of cellular shape deformation. This adaptively activates a calcium-dependent mechano-transduction pathway, controlling the level of actomyosin contractility and migration plasticity. Our data support that the nucleus establishes a functional module for cellular proprioception that enables cells to sense shape variations for adapting cellular behaviour to their microenvironment.

One Sentence Summary The nucleus functions as an active deformation sensor that enables cells to adapt their behavior to the tissue microenvironment.

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Posted December 05, 2019.
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The nucleus measures shape deformation for cellular proprioception and regulates adaptive morphodynamics
Valeria Venturini, Fabio Pezzano, Frederic Català Castro, Hanna-Maria Häkkinen, Senda Jiménez-Delgado, Mariona Colomer-Rosell, Mónica Marro Sánchez, Queralt Tolosa-Ramon, Sonia Paz-López, Miguel A. Valverde, Pablo Loza-Alvarez, Michael Krieg, Stefan Wieser, Verena Ruprecht
bioRxiv 865949; doi: https://doi.org/10.1101/865949
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The nucleus measures shape deformation for cellular proprioception and regulates adaptive morphodynamics
Valeria Venturini, Fabio Pezzano, Frederic Català Castro, Hanna-Maria Häkkinen, Senda Jiménez-Delgado, Mariona Colomer-Rosell, Mónica Marro Sánchez, Queralt Tolosa-Ramon, Sonia Paz-López, Miguel A. Valverde, Pablo Loza-Alvarez, Michael Krieg, Stefan Wieser, Verena Ruprecht
bioRxiv 865949; doi: https://doi.org/10.1101/865949

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