ABSTRACT
Tartary buckwheat (Fagopyrum tataricum) is a kind of annually herbaceous crop with higher content of rutin and flavonoids than other crops. Through transcriptome analysis to reveal the differentially expressed genes in salt pathway, the salt tolerance mechanism of Tartary buckwheat was studied. Two varieties and two new strains of Tartary buckwheat were used as experimental materials, and 100 mM was used as concentration of NaCl for treatment. We de novo assembled the reads into a transcriptome dataset containing 62,637 unigenes with N50 length of 1596 bp. A total of 205.23 million clean reads were obtained, and 33,495 (53.47%) of them were annotated. GO and KEGG classification suggested that the enrichment of these unigenes was in 47 sub-categories, 25 KOG functional categories and 129 pathways, respectively. By comparisons among 11 groups, we got many differentially expressed genes (DEGs) of transcriptome data in 8 samples. The GO classification showed that many DEGs between samples encoding for biological process, cellular component and molecular function pathway. These processes may be related to salt tolerance mechanism in Tartary buckwheat, which will provide valuable reference genetic information for the study on salt tolerance mechanism in plants and cultivating strong salt-tolerant varieties of Tartary buckwheat for more yield in saline-alkali soil. In addition, the relative expression of Unigene0009545 and Unigene0057231 in M1 and that of Unigene0023096 and Unigene0060321 in M2 was increased significantly under salt stress. We speculate that these four genes of new strains in Tartary buckwheat may be related to salt tolerance.
SUMMARY Heading Transcriptome analysis of Tartary buckwheat
Footnotes
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