Freeze Dried extracts of Bidens biternata (Lour.) Merr. And Sheriff. show significant Antidiarrheal activity in ‐vivo Models of Diarrhea

Ethnopharmacological relevance of the study Diarrhea remains one of the main killers of children aged below five years. Traditional antidiarrheal remedies form a potentially viable source of novel low cost efficacious antidiarrheal remedies in low resource settings. There is therefore a pressing to scientifically evaluate these remedies. Aim of the study This study aimed to investigate the in vivo and in vitro antidiarrheal activity of Bidens biternata a herb species used in traditional Ayurvedic medicine in the management of diarrhea. Materials and Methods In the castor oil test twenty (20) adult Sprague-Dawley rats were randomized to the negative control (normal saline), positive control (5 mg/kg loperamide), (200 mg/kg Bidens biternata extract) and (400 mg/kg Bidens biternata extract) groups (n=5 in each group). Castor oil (4 ml/kg) was then administered to the animals one hour after administration of the respective treatments after which the total mass of fecal output excreted after four (4) hours was determined. In the charcoal meal test fifteen (15) Sprague Dawley rats were randomized to the negative control (normal saline 5 ml/kg orally), the positive control (atropine sulphate 0.1 mg/kg i.p) and test (400 mg/kg Bidens biternata extract) groups (n=5). Charcoal meal was then administered via oral gavage to each rat thirty (30) minutes after the administration of the various treatments. The distance covered by the charcoal meal from the pylorus was then determined after sacrifice of the animals. In the enteropooling test twenty (20) Sprague-Dawley rats were randomized to the negative control (5% v/v ethanol in normal saline), positive control (5 mg/kg loperamide) and test (400 mg/kg Bidens biternata extract) groups and prostaglandin E2 (PGE2) (100μg/kg) administered immediately after the treatments. The animals were then sacrificed half an hour later and the volume of the small intestine contents determined. The effects of different concentrations of Bidens biternata extract (0.5. 1.0, 2.0, 3.0 and 5.0 mg/ml) on jejunal contraction were investigated and a dose-response curve constructed using the experimental data after which The ED50 dose determined. The effect of tamsulosin (α1 adrenergic blocker), yohimbine (α2 adrenergic blocker), propranolol (β adrenergic blocker) and naloxone (μ opioid blocker) on the contractile activity of the extract were also investigated. The experimental data were expressed as mean ± standard error of mean (SEM) and then analyzed using one way ANOVA followed by Tukey’s post hoc test in cases of significance (set at p<0.05). Results The freeze dried extracts of Bidens biternata had significant antidiarrhealeffects in the castor oil induced diarrhea model (p=0.0075) with maximal activity being observed at the 400mg/kg dosage level (1.66± 0.81g vs. 4.54 ± 0.51 g negative control, p=0.01). Bidens biternata extract had significant effects on intestinal motility in the charcoal meal test compared to the control group (43.61 ± 4.42% vs. 60.54 ± 3.33%: p= 0.02). Bidens biternata extract had a significant effect on PGE2 induced enteropooling (3.06 ± 0.07 ml vs. 4.74 ± 0.10 ml; p<0.001). The freeze dried extracts of Bidens biternata had a significant negative effect on the contractility of the isolated rabbit jejunum (p<0.001). The effects of the extract were significantly attenuated by tamsulosin (53.94 ± 4.20% vs. 80.57 ± 4.09%; p=0.0067) and naloxone (53.94 ± 4.20% vs. 73.89 ± 7.26 %; p=0.0358). Yohimbine (p=0.4598) and propranolol (p=0.5966) however did not have any significant effect on the contractile activity of the extract. Conclusions The freeze dried extract of Bidens biternata possess significant antidiarrhealactivity in both in vitro and in vivo models which appears to be mediated by modulating both the intestinal motility as well as the secretory activity. The results of this study also validate its traditional use as an antidiarrheal remedy.

was then administered via oral gavage to each rat thirty (30) minutes after the administration of the various treatments. The distance covered by the charcoal meal from the pylorus was then determined after sacrifice of the animals.
In the enteropooling test twenty (20) Sprague-Dawley rats were randomized to the negative control (5% v/v ethanol in normal saline), positive control (5 mg/kg loperamide) and test (400 mg/kg Bidens biternata extract) groups and prostaglandin E2 (PGE2) (100μg/kg) administered immediately after the treatments. The animals were then sacrificed half an hour later and the volume of the small intestine contents determined.
The effects of different concentrations of Bidens biternata extract (0.5. 1.0, 2.0, 3.0 and 5.0 mg/ml) on jejunal contraction were investigated and a dose-response curve constructed using the experimental data after which The ED50 dose determined. The effect of tamsulosin (α1 adrenergic blocker), yohimbine (α2 adrenergic blocker), propranolol (β adrenergic blocker) and naloxone (μ opioid blocker) on the contractile activity of the extract were also investigated.
The experimental data were expressed as mean ± standard error of mean (SEM) and then analyzed using one way ANOVA followed by Tukey's post hoc test in cases of significance (set at p<0.05).

Conclusions
The freeze dried extract of Bidens biternata possess significant antidiarrhealactivity in both in vitro and in vivo models which appears to be mediated by modulating both the intestinal motility as well as the secretory activity. The results of this study also validate its traditional use as an antidiarrheal remedy.

INTRODUCTION.
Diarrhoea continues to be one of the leading causes of death worldwide with areas within the developing world bearing a disproportionate share of the disease burden (Bartolome et al., 2013;Lopez et al., 2006) . Indeed it is now documented as the second leading cause of death in children under five accounting for over 1.5 million deaths annually (Kotloff et al., 2013;Walker et al., 2013). Diarrhea is now also recognized also a leading cause of morbidity and mortality among the elderly and HIV infected persons (Balemba

Enteric opioid receptors
Other possible mechanisms . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ;https://doi.org/10.1101https://doi.org/10. /046185 doi: bioRxiv preprint et al., 2010Kosek et al., 2003). There is a therefore a pressing need for the introduction of novel efficacious and less toxic anti-diarrhea treatments.
The World Health Organization states that traditional medicine (TM) is either the mainstay of health care delivery or serves as a complement to it in many parts of the world (Bartolome et al., 2013). There is therefore a pressing need to evaluate traditional medicine therapies for their efficacy as well as possible toxicity.
Plant species from the genus Bidens which is a member of the Asteraceae (formerly called Compositae) family are widely used in a variety of traditional medicine systems including Traditional Chinese Medicine (Bo et al., 2012), Aryuvedic medicine (Bartolome et al., 2013;Geissberger and Séquin, 1991)and also in South American traditional medicine (Geissberger and Séquin, 1991;Rabe and van Staden, 1997). Plant species from this genus are often used in the treatment of a variety of gastrointestinal conditions e.g. diarrhea, abdominal pain and even dysentery. Scientific evaluation of a number of plant species has validated their antidiarrheal activity e.g. Bidens pilosa L. var. radiata (YADAV et al., 2009) , Bidens odorata (Zavala-Mendoza et al., 2013) (Astudillo-Valdez et al., 2008. The foregoing discussion explains the rationale for this study which investigated the antidiarrheal activity of this plant species and the potential mechanisms of this activity. This study aimed at evaluating the efficacy of Bidens biternata used in traditional medicine of Paniya and Kattunaayika tribes of Waynadu Districts in Kerala as well in . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ;https://doi.org/10.1101/046185 doi: bioRxiv preprint Traditional Chinese medicine the management of diarrhea (Dharmananda, n.d.;Rana and Datt, 1997;Sukumaran et al., 2012). The plant material was air dried for one week after which it was milled to a fine powder using a standard kitchen blender. The resulting powder (500 g) was then macerated with distilled water in a 1:10 (weight: volume) ratio i.e. using five (5) liters of water for half an hour. The resulting suspension was then successively filtered using cotton wool and Whatman's filter paper respectively. The resulting filtrate was then lyophilized. The freeze dried extract formed was then weighed, placed in amber colored sample bottles and then refrigerated and retrieved as required.

Animals used
Adult Sprague-Dawley rats aged between six (6) and eight (8) weeks and weighing between two hundred (200) and four hundred (400) g were used in the in vivo studies.
. CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint Male adult New Zealand white rabbits (N = 6) weighing 2-2.5kg were used in the ex in vivo (isolated jejunum) studies. The animals were housed in the Department of Medical Physiology, University of Nairobi at standard temperature and humidity conditions and 12 hour light/dark cycle. They were fed on food and water ad libitum. They were allowed to acclimatize for 2 weeks before being used for the experiments. In the absence of an Institutional Animal Use and Care Committee (IAUCC), permission for carrying out the study was obtained from the departmental research and postgraduate studies committee.
All the experimental procedures used in the study complied with the National Institute of Health (NIH) Guidelines for the care and use of laboratory animals 8th Edition ("Guide for the Care and Use of Laboratory Animals: Eighth Edition | The National Academies Press," n.d.) and obeyed the 4Rs (reduction, replacement, refinement and rehabilitation) principles in ethical experimental design.

Castor Oil Induced Diarrhea
The antidiarrheal activity was investigated according to the method described by Zavala-Mendoza (Zavala-Mendoza et al., 2013). Briefly, twenty (20) rats were randomized to the negative control (normal saline), positive control (5 mg/kg loperamide), low dose test (200 mg/kg Bidens biternata extract) and high dose test (400 mg/kg Bidens biternata extract) groups (n=5 in each group). The treatments were administered by oral gavage.
Castor oil (4 ml/kg) was then administered to the animals one hour after administration of the respective treatments also via oral gavage.
The animals were then placed singly in separate cages lined with pre-weighed greaseproof paper. The total mass of fecal output excreted after 4 hours was measured by . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. subtracting the mass of the pre-weighed paper from the total mass of paper and fecal droppings. The results were expressed as a percentage of control according to the model described by Zavala-Mendoza ((Zavala-Mendoza et al., 2013) Charcoal meal test The effect of the extract on normal small intestinal transit was investigated using the charcoal meal method as described by Meite (Meite et al., 2009). Briefly, fifteen (15) Sprague Dawley rats were randomized to the negative control (normal saline 5 ml/kg orally), the positive control (atropine sulphate 0.1 mg/kg i.p) and test (400 mg/kg Bidens biternata extract) groups (n=5). The treatments were all administered using oral gavage.
Charcoal meal (10% activated charcoal in 5% gum acacia) (1 ml per rat) was then administered via oral gavage to each rat thirty minutes after the administration of the various treatments. The animals were then sacrificed after half an hour by cervical dislocation, the abdomen cut open and the entire small intestine from the pylorus to the caecum was isolated. The distance covered by the charcoal meal from the pylorus was then measured and expressed as a percentage of the total length of the small intestine.

Prostaglandin E2 induced enteropooling Test
This test was performed using the method described by Robert (Robert et al., 1976).
Briefly twenty (20) Sprague-Dawley rats were randomized to negative control (5% v/v ethanol in normal saline), positive control (5 mg/kg loperamide) and test (400 mg/kg Bidens biternata extract). Prostaglandin E2 (PGE2) dissolved in a vehicle consisting of 5% v/v ethanol in normal saline was then administered immediately after the treatments at a dose of 100 μg/kg. The animals were then sacrificed half an hour later by cervical . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint dislocation. The contents of entire small intestine from the pylorus to the caecum were then milked into a beaker and their volume determined.

Statistical analysis
In all the in vivo studies, the experimental data were expressed as mean ± standard error of mean (SEM). The experimental data was then analyzed statistically using one way ANOVA followed by Tukey's post hoc test in cases of significance (set at p<0.05) using the Megastat® statistical add-in for Microsoft Excel.

Isolated Rabbit Jejunum Motility
Five rabbits which had been fasted overnight were used for the study. The rabbits were euthanized by stunning (striking the base of the head in the occipital region) followed by exsanguination. The abdomen was then cut open and a ten (10) cm segment of jejunum isolated and dipped in a beaker containing cold Tyrode's solution (4°C). The contents of the jejunum were flushed out using the Tyrode solution after which the stretch of jejunum was transferred to another beaker containing Tyrode's solution at 37°C. A segment of jejunum about two (2) centimeters long was then suspended in an organ bath containing the Tyrode's solution maintained at 37 ± 1 °C and aerated with carbogen® gas (95% oxygen and 5% carbon dioxide). The upper end of the jejunum segment was hooked to an isometric force transducer (ML500/A, AD instruments) connected to a Powerlab data acquisition system (Powerlab 8/30, AD Instruments) . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint The jejunum strips were first allowed to equilibrate for an hour before the effects of different concentrations of Bidens biternata extract (0.5. 1.0, 2.0, 3.0 and 5.0 mg/ml) on jejunal contraction were investigated. A short record (about 2 min) of normal jejunal activity was recorded before administering each treatment. Starting with the lowest concentration, a one (1) mililiter dose of extract was added to the organ bath and its effect recorded for 3-5 minutes before washout. The organ bath was then rinsed twice with fresh Tyrodes solution and the jejunal strip allowed to regain its baseline activity before administration of the next dose.
The rate and force of jejunal contractions were recorded and analyzed using Chart5® software (AD Instruments). The contraction rate was expressedd as cycles per minute (cpm) while the force of contraction was expressed in grams (g). The effects of the treatments were then expressed in the manner described by Grasa (Grasa et al., n.d.) ; i.e. activity after treatment/ activity before treatment *100% And a dose-response curve then constructed. The ED50 dose was then determined from the curve

Mechanism of Action Experiments
The effect of 1 mg/ml Bidens biternata extract on jejunal activity was evaluated in the presence of various standard antagonists (at a concentration of 10 -6 M); tamsulosin (α1 adrenergic blocker), yohimbine (α2 adrenergic blocker), propranolol (β adrenergic blocker) and naloxone (μ opioid blocker) in an attempt to try and elucidate the possible mechanism(s) of its effects on jejunal activity. Normal jejunal activity was first recorded for two (2) minutes prior to the addition of the antagonist. Jejunal activity after the . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint addition of the antagonist was also recorded further two (2) minutes after which the extract was added and the resulting jejunal activity recorded for a further five (5) minutes before washout. All the experiments were repeated five times.
NB: All the concentrations quoted above were as per the final bath volume (80 ml).
The experimental data were expressed as mean ± standard error of mean (SEM). The experimental data was then analyzed statistically using one way ANOVA followed by Tukey's post hoc test in cases of significance (set at p<0.05) using the Megastat® statistical add-in for Microsoft Excel.
. CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint

Castor oil induced diarrhea
The freeze dried extracts of Bidens biternata showed significant antidiarrheal effects in the castor oil induced diarrhea model (p=0.0075). Post-hoc analysis using the Tukey test showed that the activity was dose dependent with greater activity being observed at the 400mg/kg dosage level (1.66± 0.81g vs. 4.54 ± 0.51 g negative control, p=0.01).
However the extract did not possess significant antidiarrheal activity at the 200mg/kg dosage level (p= 0.18). Hence the 400mg/kg dosage of extract was used in the next in vivo experiments. The ED50 was calculated to be 316mg/kg. The experimental data is shown in table 1.
. CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint

PGE2 induced enteropooling
Pretreatment with Bidens biternata extract had a significant effect on PGE2 induced enteropooling compared to control (3.06 ± 0.07 ml vs. 4.74 ± 0.10 ml; p<0.001). The experimental data is shown in table 3.

Effects of freeze dried extract of Bidens biternata (BBE) on spontaneous contractions of the rabbit jejunum
The freeze dried extracts of Bidens biternata had a significant effect on the contractility of the isolated rabbit jejunum (p<0.001). The dose effect on contractility was biphasic with decreases in contractility being observed up to the 3 mg/ml dosage level (42.55 ± 6.26 % of control) after which there was a paradoxical increase in contractility with maximal contractility being observed at 5 mg/ml (61.12 ± 4.30 % of control) dosage level. The experimental data obtained is shown in table 4. The 1mg/ml dose was subsequently used in the mechanism of action experiments.

Effect of antagonists on the inhibitory activity of BBE on the spontaneous contractions of the isolated rabbit jejunum
The negative effects of the extract at the 1.0mg/ml dosage level were significantly attenuated by tamsulosin (53.94 ± 4.20% vs. 80.57 ± 4.09%; p=0.0067%) and naloxone . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ;https://doi.org/10.1101/046185 doi: bioRxiv preprint (53.94 ± 4.20% vs. 73.89 ± 7.26 %;p=0.0358). Yohimbine (p=0.4598) and propanolol (p=0.5966) did not have any significant effects on the effects of the extract on the contractility of the isolated jejunum. The experimental data is shown in table 5..

DISCUSSION
The number of deaths owing to diarrhea remains unacceptably high despite the availability of treatments such oral rehydration salts (ORS), which prevent and treat dehydration, and zinc supplementation, which decreases the severity and duration of diarrhea, and antibiotics for dysentery (Walker et al., 2013). The foregoing discussion underscores the need to discover novel and more efficacious remedies. Herbal remedies which are widely used in the management of diarrhea owing to their affordability, availability and efficacy are a potentially viable source of these new remedies (Sarin and Bafna, n.d.). In addition, herbal remedies also contain multiple chemical constituents which often act synergistically (Gilani and Rahman, 2005;Mehmood et al., 2011).
The freeze dried extract of Bidens biternata had significant activity in the castor oil induced diarrhea model which is a model of secretory diarrhea coupled with altered intestinal motility (Zavala-Mendoza et al., 2013). Ricinoleic acid is released by intestinal lipases after oral ingestion of the castor oil (IWAO and TERADA, 1962) induces diarrhea through a number of mechanisms.
These include; promotion of Nitric Oxide Synthase activity (Capasso et al., 1986), involvement of endogenous tachykinins (Tiziano et al., 1997), the activation of histamine and serotonin release (Capasso et al., 1986;Gaginella et al., n.d.) among others. Niemegeers (Awouters et al., 1978) suggested that intestinal prostaglandin release was partially involved after they observed that pretreatment with NSAIDs delayed the onset of castor oil induced diarrhea without a significant effect on the fecal output. In addition, ricinoleic acid has been shown to bind to EP3 prostaglandin receptors (Tunaru et al., 2012) and this is then followed by the stimulation of neural . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint reflexes leading to increased motility. The putative mechanisms by which castor oil induces diarrhea are summarized in figure 2. Since the mechanisms by which the extract mediated its antidiarrheal activity were unclear, it was then evaluated in the PGE2 enteropooling assay. . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint

Figure 2: Putative mechanisms of the laxative effects of castor oil
The extract had significant inhibitory effects in the Prostaglandin E2 induced enteropooling model. Prostaglandin E2 is a potent intestinal secretagogue that stimulates cyclic AMP dependent Chloride ion secretion at high doses (Barrett and Keely, 2000;Field, 2003). These effects are mediated via intestinal epithelial EP2 receptors coupled to the Gs family of G proteins (Hosoda et al., 2002;Karaki and Kuwahara, 2004). It can therefore be inferred that the extract contained chemical components that had inhibitory effects on net fluid secretion in the small intestine.
The extract significantly inhibited the movement of charcoal meal. Since charcoal meal is an indigestible substance which distends the intestinal lumen thereby stimulating peristaltic movements (Zavala-Mendoza et al., 2013), this indicates that some of the chemical components of the extract had antimotility effects. The reduction in intestinal motility would potentially allow more time for intestinal fluid and electrolyte reabsorption thereby reducing fluid losses and consequently the fecal output (Sisson, 2011).
It is clear from the foregoing discussion that the extract mediates the observed antidiarrheal effects by modulating both the intestinal secretory and motile activity. The in vitro experiments . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ;https://doi.org/10.1101/046185 doi: bioRxiv preprint aimed to elucidate the putative mechanism of action(s) of the observed antimotility activity observed in the vivo models.
The significant antimotility effects of the extract on the isolated rabbit jejunum were attenuated by pretreatment with tamsulosin (α1 adrenergic receptor blocker) and naloxone (nonselective opioid receptor blocker). The antimotility effects were however insensitive to pretreatment with either yohimbine (α2 antagonist) or propanolol (nonselective β adrenergic receptor blocker). it can therefore be deduced that the antimotility activities of the extract are mediated by chemical components of the extract that possess selective agonist activity at α1 adrenoceptors and others that have selective agonist activity at opioid receptors.
It is a well-established fact that the sympathetic nervous system modulates gut muscle motility. α1 adrenoceptors are mainly coupled to the Gq/11 family of G proteins but may also be coupled to the pertussis toxin sensitive Gi protein in certain tissues (Piascik and Perez, 2001). Although α1 receptor agonists trigger smooth muscle contraction in most types of smooth muscle including vascular smooth muscle and sphincters (Garc??a-Sáinz et al., 2000), receptor activation in intestinal smooth muscle causes a decrease in contractility (Rusko and Bauer, 1988).
Activation of the α1receptors causes an increase in the intracellular calcium concentration either by triggering release from intracellular stores via the DAG/IP3 second messenger system or by causing calcium influx into the muscle cells via activation of voltage dependent and voltage independent calcium channels (Piascik and Perez, 2001) . The increase in intracellular calcium concentration would ordinarily be expected to lead to contraction. However, it has been shown that there are two calcium distribution compartments in a smooth muscle cell: a contractile compartment centrally located within the cytosol and a non-contractile compartment located between the sarcoplasmic reticulum and the plasmalemma (Karaki et al., 1997; . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint Kuwahara, 2004) . Agonists that stimulate calcium increment in the contractile compartment trigger smooth muscle contraction while those that trigger calcium increment in the noncontractile compartment trigger other calcium dependent responses such as those related to the cell membrane integrity and permeability (Karaki et al., 1997;Karaki and Kuwahara, 2004).
Indeed, Calcium channel blockers and the absence of extracellular calcium have been found to inhibit the α1 mediated effects on intestinal motility, exemplifying the role of calcium in the inhibition of intestinal smooth muscle motility by α1 receptor agonists (Karaki et al., 1997)..
In addition, the gut inhibitory response to phenylephrine an α1 adrenergic receptor agonist, has been shown to be sensitive to membrane stabilizers such as quinidine and procaine suggesting that its actions may involve alterations in membrane permeability (BOWMAN and HALL, 1970). The activity of phenylephrine was on gut motility was shown to be sensitive to apamine (an inhibitor of small conductance calcium-activated channels) (Romanelli et al., 2003) and tetraethylammonium (Rusko and Bauer, 1988). Small conductance calcium-activated channels (SKCa) are voltage-independent potassium channels sensitive to submicromolar concentrations of calcium (Xia et al., 1998). It can therefore be postulated that small increases in intracellular calcium concentration following the activation of the α1 adrenoceptors, would activate these ion channels leading to an efflux of potassium leading to hyperpolarization of the muscle cell and consequently muscle relaxation. This hypothesis would also explain the increase in motility seen at higher doses of extract in the in-vitro experiments.
Naloxone which significantly attenuated the effects of the extract is a nonselective opioid receptor blocker though it has greater affinity for the μ subtype (Holzer, 2009). Opioid receptors are primarily located within the Enteric Nervous System in the gut (Galligan and Akbarali, 2014).
Their activation leads to a decrease in neuronal excitability via a variety of mechanisms including; . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint inhibition of adenylyl cyclase, inhibition of Ca 2+ influx and activation of K + efflux (Al-Hasani and Bruchas, 2011;Galligan and Akbarali, 2014). The reduced excitability in turn causes a reduction in neurotransmitter release in the enteric nervous system e.g. decreased Acetylcholine release from excitatory interneurons, increased NO/purines release from inhibitory motor neurons and decreased Acetylcholine/Vasoactive Intestinal Peptide release from submucosal secretomotor neurons (Galligan and Akbarali, 2014) . This then leads to the inhibition of propulsive intestinal motility as well as the inhibition of mucosal chloride ion secretion (Sobczak et al., 2014). It can therefore be postulated that the antidiarrheal actions of the extract may be mediated by binding of some of its chemical constituents to opioid receptors. Although the mechanism of action experiments were carried out using an ex in-vivo model it can be clearly seen that the antisecretory actions of opioid receptor activation can be reasonably extrapolated to explain the antisecretory activity observed in the enteropooling model.
It can then be postulated that the antidiarrheal effects of the extract were mediated by agonism at both the α1 adrenoceptors and the µ opioid receptors. Activity at the α1 adrenoceptors resulted in the inhibition of motility while activity at the opioid receptors was involved in the inhibition of both the motility and secretion.
Preliminary phytochemical analysis of Bidens biternata has confirmed the presence of various types of chemical compounds which include phenols, flavonoids, tannins, glycosides and free fatty acids (Sukumaran et al., 2012). In addition, this plant species is used as a leafy vegetable attesting to its nutritional properties which have also been scientifically documented (Sukumaran et al., 2012). This therefore increases its utility in the management of diarrhea that is comorbid . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint with a malnutrition a common situation in the tropics. Future studies will try to identify the specific chemical moieties responsible for the antidiarrheal effects observed in this study.

CONCLUSIONS
In conclusion, the results obtained in this study indicate that the freeze dried extract of Bidens biternata possess significant antidiarrheal activity inin vivo models of diarrhea. The antidiarrheal activity is mediated by the modulating both the intestinal motility as well as the secretory activity. The results of this study also validate its traditional use as an antidiarrheal remedy.
Future studies should focus on further evaluation of this plant as a potential source of low cost and accessible antidiarrheal drug moieties . CC-BY 4.0 International license a certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under The copyright holder for this preprint (which was not this version posted March 29, 2016. ; https://doi.org/10.1101/046185 doi: bioRxiv preprint