Evolution environment Clone number Fitness at 21 C vs. original reference Fitness at 21 C vs. cured reference Evolved in SC clone1 -0.04287380725 0.270380717068 Evolved in SC clone10 -0.0201725463483 0.330655971448 Evolved in SC clone11 -0.0658281412976 -0.043487298795 Evolved in SC clone12 -0.0286163795326 0.207637757257 Evolved in SC clone13 -0.218351379604 -0.0600921734155 Evolved in SC clone14 -0.0519154063054 -0.0356138443722 Evolved in SC clone15 -0.0155616762322 -0.0290452847732 Evolved in SC clone16 -0.012867764396 -0.0335503280243 Evolved in SC clone17 -0.285093349782 -0.0482354564621 Evolved in SC clone18 -0.0135204749494 -0.0347318996867 Evolved in SC clone19 -0.0211642418956 -0.0340264249829 Evolved in SC clone2 -0.0298060106511 -0.0494529748735 Evolved in SC clone20 -0.231114381955 -0.029491360592 Evolved in SC clone3 -0.020094872095 0.33068288854 Evolved in SC clone4 -0.265222406333 -0.0512373982933 Evolved in SC clone5 -0.0260605363811 0.333580155184 Evolved in SC clone6 -0.311310212362 -0.0339003898481 Evolved in SC clone8 -0.0287187021561 -0.0373647190589 Evolved in SC clone9 -0.0308393685389 -0.0457524568841 Evolved in gal clone1 -0.0314571927496 0.213997015755 Evolved in gal clone10 -0.0300662672709 0.276260062818 Evolved in gal clone11 -0.0336856348071 -0.050580419027 Evolved in gal clone12 0.0107207024186 -0.0459337067597 Evolved in gal clone13 -0.0322669026129 0.130851228106 Evolved in gal clone14 -0.0516809129293 -0.0643694215945 Evolved in gal clone15 -0.0399380587729 -0.0487462502165 Evolved in gal clone17 -0.0288084108676 -0.060783087988 Evolved in gal clone18 -0.0233525448422 -0.0388937467782 Evolved in gal clone19 -0.0652764913044 -0.0456722773149 Evolved in gal clone2 -0.0344816190801 0.114420694488 Evolved in gal clone20 -0.0654050366861 -0.0348554377002 Evolved in gal clone3 -0.0450565017538 -0.0527373583226 Evolved in gal clone4 -0.0533407202371 0.146591152763 Evolved in gal clone5 -0.142859647234 -0.136447303851 Evolved in gal clone6 -0.0149169633668 0.12852566353 Evolved in gal clone7 -0.0445080702576 -0.0701344082306 Evolved in gal clone8 -0.0559665861079 -0.0389921845105 Evolved in gal clone9 -0.286303502694 -0.0373699368481 Evolved in 21 C clone1 -0.0365317810708 -0.0637138009923 Evolved in 21 C clone10 -0.324990293395 -0.0530704317464 Evolved in 21 C clone11 -0.0234110649019 0.200186561262 Evolved in 21 C clone12 -0.0204226514293 -0.0124981176769 Evolved in 21 C clone13 0.00323149523009 0.0523244170946 Evolved in 21 C clone14 0.00457164279662 0.344462192003 Evolved in 21 C clone16 -0.0381504905388 -0.0598753014607 Evolved in 21 C clone17 -0.00821061624782 -0.0291984006149 Evolved in 21 C clone18 -0.0285748818188 0.0701240784561 Evolved in 21 C clone19 -0.0178134861562 -0.0435523068247 Evolved in 21 C clone2 -0.0360854754833 -0.0528847814178 Evolved in 21 C clone20 -0.0292669408351 0.0784077141723 Evolved in 21 C clone3 -0.0317519486365 -0.0565523868845 Evolved in 21 C clone4 0.0196226732671 0.274943346953 Evolved in 21 C clone5 -0.0247561733652 0.103086495799 Evolved in 21 C clone6 -0.000100679224908 0.211307618679 Evolved in 21 C clone7 -0.007422766227 -0.0258582677005 Evolved in 21 C clone8 -0.293351966505 -0.0256587659987 Evolved in 21 C clone9 -0.00793031465887 0.186087647562 Evolved in 37 C clone1 -0.208113214886 -0.0742730552106 Evolved in 37 C clone10 -0.241987043826 -0.0661164468256 Evolved in 37 C clone11 -0.269353178509 -0.068810011236 Evolved in 37 C clone12 -0.0364375838616 -0.0542023322939 Evolved in 37 C clone13 -0.294373948158 -0.0660032819224 Evolved in 37 C clone14 -0.232220964763 -0.0536618838931 Evolved in 37 C clone15 -0.318373964268 -0.102687112107 Evolved in 37 C clone16 -0.308069523691 -0.0814488423032 Evolved in 37 C clone17 -0.036445436636 0.180903473726 Evolved in 37 C clone18 -0.232059181679 -0.0475104370493 Evolved in 37 C clone19 -0.274740091067 -0.0510278464782 Evolved in 37 C clone2 -0.231506876326 -0.0599370406482 Evolved in 37 C clone3 -0.282627687076 -0.0561172115224 Evolved in 37 C clone4 -0.250372482811 -0.0422572484482 Evolved in 37 C clone5 -0.0444104936941 0.2242855117 Evolved in 37 C clone6 -0.273567212824 -0.0702208969718 Evolved in 37 C clone7 -0.229709640342 -0.0572729835337 Evolved in 37 C clone8 -0.0809049981127 -0.0697072628974 Evolved in 37 C clone9 -0.108703002268 -0.0766162072782 Evolved in .2 M clone1 -0.0295346425693 0.0947283762781 Evolved in .2 M clone10 -0.0404293839905 0.203718069297 Evolved in .2 M clone11 -0.0379063910893 0.0555879091293 Evolved in .2 M clone12 -0.0138876706314 -0.0458921288908 Evolved in .2 M clone13 -0.0386381256761 0.061663871757 Evolved in .2 M clone14 -0.0218047672796 0.184666504303 Evolved in .2 M clone15 -0.0523273350565 -0.0590108603184 Evolved in .2 M clone16 -0.0240090944947 0.201747665466 Evolved in .2 M clone17 -0.0243625331972 0.356096825368 Evolved in .2 M clone18 -0.0585788486784 0.259895254971 Evolved in .2 M clone19 -0.336645191175 -0.0340936485675 Evolved in .2 M clone2 -0.338311782591 -0.0348671658204 Evolved in .2 M clone20 -0.0451897933353 -0.0602174271862 Evolved in .2 M clone3 -0.283601339403 -0.0576075160198 Evolved in .2 M clone4 -0.324015233273 -0.0370588592783 Evolved in .2 M clone5 -0.0317035964146 0.227311109387 Evolved in .2 M clone6 -0.294591679966 -0.044071632621 Evolved in .2 M clone7 -0.0368628852089 -0.0475035448997 Evolved in .2 M clone8 -0.249254193187 -0.0522837094538 Evolved in .2 M clone9 -0.0129972959715 -0.0302207837406 Evolved in .07% glu clone1 0.0185260529269 0.0163523651837 Evolved in .07% glu clone10 -0.0985260746579 -0.110669647626 Evolved in .07% glu clone11 -0.0312743971837 0.24822077113 Evolved in .07% glu clone12 -0.0420540766742 -0.0464569434995 Evolved in .07% glu clone13 -0.0385887804872 -0.0501761453083 Evolved in .07% glu clone14 -0.191686518043 -0.0732392234385 Evolved in .07% glu clone15 -0.0093189637851 0.136487537094 Evolved in .07% glu clone16 -0.226157825421 -0.0427813673232 Evolved in .07% glu clone17 -0.0654881577756 -0.0624559512555 Evolved in .07% glu clone18 -0.0374450279578 -0.0552127407632 Evolved in .07% glu clone19 -0.253403308185 -0.0364327570643 Evolved in .07% glu clone2 -0.260398239431 -0.0344752509251 Evolved in .07% glu clone20 -0.0427643490518 -0.0425571436548 Evolved in .07% glu clone3 -0.042662071747 -0.0612504642446 Evolved in .07% glu clone4 -0.0453088264787 -0.0675714853191 Evolved in .07% glu clone5 -0.0430201371619 -0.0644922966365 Evolved in .07% glu clone6 -0.0223812141218 0.32982658724 Evolved in .07% glu clone7 -0.0331376117459 0.196671313101 Evolved in .07% glu clone8 -0.00479169080936 0.189245441736 Evolved in .07% glu clone9 -0.317141176757 -0.0351540754556 Evolved in pH 3.8 clone1 -0.0550002951662 -0.0695935152047 Evolved in pH 3.8 clone10 -0.015599761389 -0.0136435786688 Evolved in pH 3.8 clone11 -0.0113454006525 -0.0463436710181 Evolved in pH 3.8 clone12 -0.0295776692919 0.281247601161 Evolved in pH 3.8 clone13 -0.0365249902831 0.120786618044 Evolved in pH 3.8 clone14 -0.0364074820934 -0.0464391459489 Evolved in pH 3.8 clone15 -0.0442581873036 0.227024644111 Evolved in pH 3.8 clone17 -0.035492494403 -0.0447120480784 Evolved in pH 3.8 clone18 -0.0212740505636 -0.0413194053458 Evolved in pH 3.8 clone19 -0.0268075203422 -0.0470099656334 Evolved in pH 3.8 clone2 -0.0245109562962 0.210846325304 Evolved in pH 3.8 clone20 -0.0435430334195 -0.0525380846431 Evolved in pH 3.8 clone3 -0.0207859032176 0.100973846602 Evolved in pH 3.8 clone4 -0.0419088899548 0.230787584264 Evolved in pH 3.8 clone5 -0.0302689805208 -0.0555995512949 Evolved in pH 3.8 clone6 -0.219349934091 -0.0403090684298 Evolved in pH 3.8 clone7 -0.032165567332 0.213142747673 Evolved in pH 3.8 clone8 -0.0407811809499 0.192552421961 Evolved in pH 3.8 clone9 -0.0440144627857 0.209517732532 Evolved in pH 3 clone1 -0.300509458364 -0.080089645909 Evolved in pH 3 clone11 -0.0434934133902 -0.0583595946816 Evolved in pH 3 clone12 -0.114195689476 -0.0246199895211 Evolved in pH 3 clone13 -0.293484329241 -0.0539449411994 Evolved in pH 3 clone14 -0.291539158067 -0.0579750106499 Evolved in pH 3 clone16 -0.0285736369145 0.275446324663 Evolved in pH 3 clone17 -0.185358773116 -0.0348066370466 Evolved in pH 3 clone18 -0.27759515674 -0.0264602255467 Evolved in pH 3 clone19 -0.250053272338 -0.0598622984079 Evolved in pH 3 clone2 -0.396336396347 -0.0917998411964 Evolved in pH 3 clone20 -0.265020088731 -0.0277569089889 Evolved in pH 3 clone3 -0.393950416264 -0.0974799478225 Evolved in pH 3 clone4 -0.400318091557 -0.0951847726473 Evolved in pH 3 clone5 -0.0214239144337 -0.0272922909711 Evolved in pH 3 clone6 -0.214507410921 -0.0443315514658 Evolved in pH 3 clone7 -0.261429363902 -0.0548687858188 Evolved in pH 3 clone8 -0.14439935662 -0.037225812688 Evolved in pH 3 clone9 -0.236338276128 -0.0313742373282 Evolved in pH 6 clone1 -0.0236131007788 0.545596218283 Evolved in pH 6 clone10 -0.0852806545559 -0.100133591754 Evolved in pH 6 clone11 -0.0369282500061 -0.0275682167601 Evolved in pH 6 clone12 -0.0730107202733 0.27962429251 Evolved in pH 6 clone13 -0.326171504418 -0.0618510281105 Evolved in pH 6 clone14 -0.344007669328 -0.104701119344 Evolved in pH 6 clone15 -0.0698845159971 -0.0661056734624 Evolved in pH 6 clone17 -0.083711676154 -0.0447064171527 Evolved in pH 6 clone18 -0.09040054043 -0.0787067839428 Evolved in pH 6 clone19 -0.0527797079351 -0.071949346277 Evolved in pH 6 clone2 -0.0352367547625 -0.0522174259765 Evolved in pH 6 clone20 -0.0397700807604 0.197438628387 Evolved in pH 6 clone3 -0.0569785767458 -0.0765920245368 Evolved in pH 6 clone4 0.000598919494953 -0.00474767055146 Evolved in pH 6 clone5 -5.12860000009e-05 -0.02667159498 Evolved in pH 6 clone6 -0.405542739749 -0.0205919030898 Evolved in pH 6 clone7 -0.0565532978152 -0.0617017267692 Evolved in pH 6 clone8 -0.00333041641542 -0.0228969537934 Evolved in pH 6 clone9 -0.0344979384299 -0.0419206724111 Evolved in pH 7.3 clone1 -0.0172464045091 0.165429252435 Evolved in pH 7.3 clone10 -0.389211826519 -0.0632044661161 Evolved in pH 7.3 clone11 -0.362427232596 -0.132689869937 Evolved in pH 7.3 clone12 0.00640866034033 0.182901415182 Evolved in pH 7.3 clone13 -0.16466517411 -0.0414306192141 Evolved in pH 7.3 clone14 -0.276163266228 -0.0409352114113 Evolved in pH 7.3 clone15 -0.00609462583844 0.210830150247 Evolved in pH 7.3 clone16 -0.0931559973643 -0.0587470134227 Evolved in pH 7.3 clone17 -0.141597595274 -0.0408242236158 Evolved in pH 7.3 clone18 -0.369580552393 -0.0366462187271 Evolved in pH 7.3 clone19 -0.0609533706519 0.210465344326 Evolved in pH 7.3 clone2 -0.0497401164395 0.200648089036 Evolved in pH 7.3 clone20 -0.0902775509314 -0.0236137571361 Evolved in pH 7.3 clone3 -0.0338779993181 -0.0503340735393 Evolved in pH 7.3 clone4 -0.339348121047 -0.043730230075 Evolved in pH 7.3 clone5 -0.074454913976 -0.087494691032 Evolved in pH 7.3 clone6 -0.115915338799 0.250625001302 Evolved in pH 7.3 clone7 -0.384727736392 -0.0535726667599 Evolved in pH 7.3 clone8 -0.00610711949715 -0.0282645503006 Evolved in pH 7.3 clone9 -0.344148293035 -0.121961182952 Evolved in .8 M clone1 -0.364543117275 -0.0430411624243 Evolved in .8 M clone10 -0.0210996040776 0.208749484291 Evolved in .8 M clone11 -0.343596600571 -0.0389587754108 Evolved in .8 M clone12 -0.068699068046 0.210199743394 Evolved in .8 M clone13 -0.310582121419 -0.031471104891 Evolved in .8 M clone14 -0.0327950185299 0.202575071176 Evolved in .8 M clone15 -0.243176320001 -0.045841282372 Evolved in .8 M clone16 -0.29276852981 -0.0251383533151 Evolved in .8 M clone17 -0.328945058612 -0.0680596737565 Evolved in .8 M clone18 -0.0332341810281 -0.0417192953094 Evolved in .8 M clone19 -0.285974926296 -0.0725122184295 Evolved in .8 M clone2 -0.164992854372 0.0852719487309 Evolved in .8 M clone20 -0.234229546154 -0.0634473784791 Evolved in .8 M clone3 -0.0504439630654 -0.0500663402015 Evolved in .8 M clone4 -0.0209661700569 0.0653949428009 Evolved in .8 M clone5 -0.019396553409 -0.0325063736877 Evolved in .8 M clone6 -0.256311760725 -0.0288420190487 Evolved in .8 M clone7 -0.351087264173 -0.107598973622 Evolved in .8 M clone8 -0.282247183974 -0.0779289845018 Evolved in .8 M clone9 -0.238536044098 -0.0832823937633