A Pvr–AP-1–Mmp1 signaling pathway is activated in astrocytes upon traumatic brain injury

Traumatic brain injury (TBI) caused by external mechanical forces is a major health burden worldwide, but the underlying mechanism in glia remains largely unclear. We report herein that Drosophila adults exhibit a defective blood-brain-barrier (BBB), elevated innate immune responses, and astrocyte swelling upon consecutive strikes with a high-impact trauma device. RNA sequencing (RNA-seq) analysis of these astrocytes revealed upregulated expression of genes encoding PDGF and VEGF receptor-related (Pvr, a receptor tyrosine kinase (RTK)), adaptor protein complex 1 (AP-1, a transcription factor complex of the c-Jun N-terminal Kinase (JNK) pathway) composed of Jun-related antigen (Jra) and kayak (kay), and matrix metalloproteinase 1 (Mmp1) following TBI. Interestingly, Pvr is both required and sufficient for AP-1 and Mmp1 upregulation, while knockdown of AP-1 expression in the background of Pvr overexpression in astrocytes rescued Mmp1 upregulation upon TBI, indicating that Pvr acts as the upstream receptor for the downstream AP-1–Mmp1 transduction. Moreover, dynamin-associated endocytosis was found to be an important regulatory step in downregulating Pvr signaling. Our results identify a new Pvr–AP-1–Mmp1 signaling pathway in astrocytes in response to TBI, providing potential targets for developing new therapeutic strategies of TBI. Main Points The study provided RNA-seq data of astrocytes following traumatic brain injury (TBI) Genes involved in endocytic trafficking are upregulated in astrocytes after TBI A new Pvr–AP-1–Mmp1 pathway is activated in astrocytes following TBI. Inhibition of endocytosis in astrocytes upregulates the Pvr–AP-1–Mmp1 signaling.


Introduction
Traumatic brain injury (TBI) is one of the leading causes for adult disability due to limited neurological recovery (Joy et al., 2019).About 43% of people discharged after hospitalization for acute TBI develop long-term locomotor disability (Ma et al., 2014).TBI triggers primary neural injuries via rapid physical forces that damage axons, glia and blood vessels (Chodobski et al., 2011;Daneshvar et al., 2015), followed by diverse secondary damage such as Wallerian axon degeneration (Büki and Povlishock, 2006) and blood-brain barrier (BBB) dysfunction (Chodobski et al., 2011) in the central nervous system (CNS).The outcome of TBI is affected by various factors including age and diet (Byrns et al., 2021;Katzenberger et al., 2016).In addition, glial cells are critical mediators in response to TBI in the mammalian brain (Mira et al., 2021).During TBI, astrocytes become active, a process characterized by swelling, scar tissue formation, and engulfment of neuronal debris (Liddelow et al., 2017;Sofroniew and Vinters, 2010), while microglia rapidly transform to their active state to phagocytose cell debris (Davalos et al., 2005).
Drosophila melanogaster is an ideal model organism for discovering new gene networks and investigating the molecular and cellular responses to CNS injury (Ayaz et al., 2008;Bier, 2005;Hoopfer et al., 2006;MacDonald et al., 2006).Drosophila astrocyte-like glia (henceforth astrocytes), the conserved counterparts for mammalian astrocytes, and ensheathing glia (ENG) constitute the major glial subclasses in the CNS (Doherty et al., 2009;Stork et al., 2014).A previous study revealed that Drosophila ENG perform the phagocytic function by the engulfment receptor Draper which interacts with downstream signaling components, including ced-6, Shark, Src42a, c-Jun N-terminal Kinase (JNK), and Mmp1 in the glial engulfment process in response to axotomy (Awasaki et al., 2006;Doherty et al., 2014;Lu et al., 2014;Lu et al., 2017;Purice et al., 2017).Although ENG function upon axotomy has been well characterized, the response of other types of glia such as astrocytes to neural injury remains unclear.
In mammals, signaling by platelet-derived growth factor (PDGF) directs cell migration, alters cell shape, and plays a role in wound healing (Hoch and Soriano, 2003;Sil et al., 2018).Meanwhile, vascular endothelial growth factor (VEGF) signaling is critical for cell proliferation and survival, and vascular endothelial cell mitogenesis and permeability (Apte et al., 2019;Sakurai et al., 2005).Pvr, short for PDGF-and VEGF receptor-related, is the sole Drosophila ortholog of the PDGF and VEGF receptor families (Cho et al., 2002).Pvr interacts with different signaling molecules in a context-dependent manner.For example, Pvr regulates thorax closure during metamorphosis via the c-Jun N-terminal Kinase (JNK) signaling pathway (Ishimaru et al., 2004), but the Pvr-mediated wound-induced epidermal cell migration does not depend on JNK (Wu et al., 2009).The JNK/Activator Protein-1 (AP-1) signaling pathway is involved in different cellular processes, including cell migration (Pastor-Pareja et al., 2004), organ size control (Willsey et al., 2016), and cell death (Moreno et al., 2002).The JNK effector AP-1 complex, composed of heterodimeric Jun/Jun-related antigen (Jra) and Fos/kayak (kay), promotes transcription of genes including matrix metalloproteinase 1 (Mmp1) that functions in tissue remodeling, regulation of inflammatory processes, and metastasis of cancer cells (Egeblad and Werb, 2002;Page-McCaw et al., 2007;Parks et al., 2004).
Previous studies have implicated a role for ensheathing glia, Draper pathway, and the innate immune pathway in response to TBI (Doherty et al., 2009;Lu et al., 2017;Purice et al., 2017;Katzenberger et al., 2013).However, whether astrocytes also play a role in TBI response remains elusive.Herein, using a Drosophila TBI model induced with a high-impact trauma (HIT) device (Katzenberger et al., 2013), we found that TBI caused multiple defects including upregulated expression of innate immune genes and astrocyte swelling.To uncover the molecular pathways activated by TBI, we performed RNA sequencing (RNA-seq) analysis of astrocytes sorted by fluorescence-activated cell sorting (FACS) to profile TBI-induced genes.Interestingly, we showed that the expression of Pvr, AP-1, and Mmp1 was upregulated following TBI.Knockdown of Pvr or AP-1 expression in astrocytes rescued TBI-induced Mmp1 upregulation, while Pvr overexpression promoted Mmp1 expression via AP-1 upon TBI.Our findings identify a new Pvr-AP-1-Mmp1 signaling pathway that acts predominantly in astrocytes in response to TBI, and shed light on the mechanisms of astrocyte responses following TBI.

Drosophila stocks and husbandry
Flies were maintained on standard cornmeal medium at 25°C otherwise indicated.

Traumatic brain injury (TBI) Assay
Construction and use of the HIT device were described previously (Katzenberger et al., 2013).Deflection of the spring to 90°was the standard angle for strike.
Mortality index at 24 h (MI24) were determined by analyzing at least 120 flies (20 flies for each test).

Dye penetration assay
Dye penetration experiments were performed as previously described (Bainton et al., 2005).10 kD Texas red-conjugated dextran (Molecular Probes) at 2.5 mM was injected into the soft tissue between two abdominal segments of the exoskeleton of flies with and without TBI.Flies were allowed to recover in fresh food tubes and photographed 2 h later on a Zeiss microscope to detect the integrity of brain-eye barrier (BEB).To detect the integrity of BBB, fly brains were dissected at 2 h after injection and examined under an Olympus FluoView FV1000 microscope.

MARCM analysis
Mosaic analysis with a repressible cell marker (MARCM) analysis in astrocytes was carried out largely following previous publications (Jia et al., 2019;Lee and Luo, 1999).Specifically, newly laid eggs were collected for a 4-5 h interval and cultured at 18°C.Clones were induced by heat shock at 24 h after larval hatching in a 37°C water bath for 15 min and flies were raised at 25°C after heat shock until immunohistochemistry analysis of adult brain.

Immunohistochemistry analysis and confocal microscopy
Brain tissues were dissected out from adult after specific treatments.Fixation and staining of adult brains were performed as previously described (Jia et al., 2019).

Astrocyte preparation for RNA-seq analysis
Flies were collected at 3 days after eclosion and treated with TBI.Brains from adult flies (R86E01-Gal4/UAS-Redstinger) with no injury, 4 h after injury and 24 h after injury were dissected out in freshly prepared adult hemolymph saline (AHS) buffer, as previously described (Yang et al., 2016).Briefly, a total of ∼50 brains for each group was immediately transferred to an Eppendorf tube (1.5 mL) containing 200 µL AHS with Pronase (1 mg/mL; P5147, SigmaAldrich) and Dispase (1 mg/mL; LS02104, Worthington Biochemical Corporation).After tissue digestion for 30 min at 25°C, the enzyme medium was removed, followed by two brief, gentle washes with fresh AHS containing 2% PBS.Brain samples were then triturated by gently pipetting in 1 mL AHS through four fire-polished Pasteur pipettes with descending pore sizes (400 μm, 300 μm, 200 μm, and 100 μm) for a total of ∼20 min.Next, the dissociated cells were centrifuged for 10 min at 100 × g and the AHS was replaced with the same buffer to minimize any RNA content released from damaged cells during the trituration process.Finally, dissociated tissues were filtered through 45 μm filter and then put on ice.FACS sorting was performed according to the following gating procedure with 70 μm nozzle by BD FACS Aria: (1) an initial SSC-A/FSC-A gate to minimize debris; (2) a SSC-A/pe-Texa Ted-A gate to choose only red fluorescence positive cells.At this step, 1600 cells were sorted directly into ice-cold cell lysis buffer (oligo-dT30VN primer, ribonuclease inhibitor, dNTP mix) and stored at -80°C until all samples were ready for RNA extraction and cDNA amplification.

cDNA library preparation
A total of 5 biological replicates were collected for each condition (no injury, 4 h after injury, and 24 h after injury).Cell samples were sent to the Anoroad Company for RNA extraction.An Oligo-dT primer was introduced to the reverse transcription reaction for first-strand cDNA synthesis, followed by PCR amplification to enrich the cDNA and magbeads purification step was used to purify the products.Briefly, Smart-Seq2 method was used to amplify cDNA.Concentration and integrity of cDNA was assessed using Qubit 3.0 Flurometer (Life Technologies) and Agilent 2100 Bioanalyzer (Agilent Technologies) to ensure the cDNA length was around 1-2 kbp.
After quality control, 40 ng cDNA was used to be fragmented at 350 bp by Bioruptor® Sonication System (Diagenode Inc.).To construct Illumina library, end repair, 3' ends A-tailing, adaptor ligation, PCR amplification and library validation were performed.PerkinElmer LabChip® GX Touch and Step OnePlus™ Real-Time PCR System were used for library quality inspection.Qualified libraries were loaded on Illumina Hiseq platform for PE150 sequencing.

RNA-seq and data analysis.
A total of 539 million reads (average 35.9 million reads per sample) was obtained.
The fastq data were first processed with Trimmomatic (Bolger et al., 2014) to remove Illumina adaptor.The remaining data was aligned against the UCSC D. melanogaster genome (dm6) using Hisat2 (Kim et al., 2015;Kim et al., 2019;Pertea et al., 2016) with the annotation file of the same version.On average, 92% of the initial reads were mapped to dm6.HTSeq was used to calculate the raw read counts of genes for each sample.DESeq2 (Love et al., 2014) was used to normalize the data.Transcripts were considered as significantly differentially expressed if their adjusted p values were less than 0.01 (p < 0.01) by the Benjamini-Hochberg method.Following normalization of the data, DESeq2 was used to perform pairwise comparisons between the control and experimental groups using parametric tests.Genes with absolute value of logarithmic fold change (log2FC) > 1.0 were qualified for the final set of DEGs.Volcano plots and heatmap were constructed for visualization of the resulting expression intensity data following the identification of DEGs and RNA-Seq data normalization by the adjusted p value calculations.

Quantitative reverse transcription-PCR analysis
RT-qPCR was performed largely based on previous protocols (Zhao et al., 2020).
Flies were treated with the same conditions as the RNA-seq experiment.RNA for antimicrobial peptides (AMPs) analysis was extracted from whole fly brains by TriZol.
RNA for confirming RNA-seq findings was isolated from more than 10000 astrocytes sorted by FACS using the PicoPure RNA isolation kit (KIT0204, Life Technologies).cDNA was generated with SuperScript III First-Strand Synthesis SuperMix (11752250, Invitrogen).For each gene, at least three pairs of qPCR primers were screened by standard curve analysis, and the best primer pairs were chosen based on their efficiency and specificity.Real-time qPCR was carried out by KAPA Library Quantification Kit (KR0389, KAPA Biosystems).Primer sequences are provided in Table S1.

Thermogenetic inhibition of endocytosis
For inhibition of endocytosis in astrocytes using UAS-Shi ts , crosses were reared and treated by HIT device at 23°C to avoid unintended inactivation.For thermogenetic inactivation, flies after TBI were undergone intermittent temperature treatment at 32°C (32°C for 30 min, and then back to 23°C for 30 min, for four rounds, four hours in total) until dissection.
One-way ANOVA was used to compare multiple group means.Student's t-tests were used for statistical comparisons between two groups.

TBI is induced in Drosophila adults by a high-impact trauma (HIT) device
To analyze how brains respond to TBI, we applied consecutive strikes to adult flies using a HIT device to induce brain injury as described previously (Katzenberger et al., 2015).To quantify the degree of injury, we analyzed the mortality index at 24 h after TBI (MI24), defined as the percentage of dead over the total flies tested.
Consistent with a previous report (Katzenberger et al., 2013), MI24 of 3-day-old adult flies was 20 ± 3.18% after 4 HIT strikes (Figure 1A).MI24 after 6 strikes was 71.88 ± 7.44%, suggesting that increasing the number of strikes results in a higher MI24 rate (Figure 1A).Given that TBI has been shown to disrupt the formation of the blood-brain barrier (BBB) in mammals and flies and blood-eye barrier (BEB) in flies (Rodríguez-Baeza et al., 2003;Sangiorgi et al., 2013;Katzenberger et al., 2015;Saikumar et al., 2020), we examined BBB/BEB integrity 2 h after injecting tetramethylrhodamine-conjugated dextran (molecular weight = 10,000 Da) into adult fly abdominal segments (Bainton et al., 2005).The dye crossed the barrier and penetrated the retina and brain, indicating that both BBB and BEB were disrupted after HIT strikes (Figures 1B  G).The percentage of flies exhibiting dextran-permeable BBB increased as the number of strikes increased from 0 to 6, with six strikes resulting in severe BBB disruption (Figure 1G) but maintaining a moderate viability providing enough samples for phenotypic analysis.Based on these results, six HIT strikes administered at 5 min intervals were applied to induce TBI throughout the present study.
In addition to disruption of BBB/BEB integrity, we also analyzed TBI-induced secondary damage such as local and systemic immune responses.Given that Toll and immune deficiency (Imd) are the two crucial innate immune response pathways in Drosophila (Valanne et al., 2011), and their common downstream genes encode antimicrobial peptides (AMPs) that protect flies from TBI injury (Alphen et al., 2022), we analyzed the expression of AMPs in adult fly brains at different time points after TBI.As shown by the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) results, expression of the AMP genes AttC, DiptB and Mtk was significantly upregulated at 1 h and reached a peak at 4 h after injury (Figure 1H), consistent with previous reports (Barekat et al., 2016;Katzenberger et al., 2013).
Taken together, these results suggest that HIT strikes induce TBI reflected by BBB/BEB disruption and elevated expression of innate immune response genes.

TBI induces neuronal but not glial apoptosis in adult fly brains
Given that neurons and glia have been shown to undergo cell death during both primary and secondary injury responses upon TBI in humans (Ng and Lee, 2019), we performed immunostaining with an antibody against Death caspase-1 (Dcp-1), the homolog of human caspase-3, to detect apoptotic cells in our TBI model.Interestingly, the number of Dcp-1 + puncta was significantly increased in adult fly brains at 24 h after TBI (Figures 1I and J), with a 2.47-fold increase compared to the number in uninjured controls (Figure 1K).Furthermore, we found that almost all Dcp-1 + puncta were co-localized with Elav rather than Repo, suggesting that neurons but not glia are apoptotic (Figures 1L  M'').These results suggest that, as in mammals, TBI also induces neuronal apoptosis in fruit flies.

TBI leads to astrocyte activation
In addition to the TBI-induced neuronal apoptosis mentioned above, glial cells also become reactive after nervous system injury (Burda and Sofroniew, 2014).It has been shown that ENG phagocytose neuronal debris upon axotomy (Doherty et al., 2009), but whether astrocytes respond to TBI remains unknown.To explore the potential role of astrocytes in the TBI response, R86E01-GAL4 (Kremer et al., 2017), an astrocyte-specific driver, was used to express UAS-mCD8::GFP to label astrocyte plasma membrane.In uninjured fly brains, GFP intensities in the cell bodies and processes of astrocytes were widely distributed throughout the brain (Figures 2A and     2D).While no significant change in GFP intensity was detected in brains at 4 h after TBI (Figures 2B and 2D), the GFP intensity was significantly increased and the cell bodies of astrocytes were significantly enlarged at 24 h after TBI in the central brain region, especially in the antennal lobes (ALs) (Figures 2C and 2D).Of note, these results were not affected by the presence of one or two UAS transgenes as the efficiency of R86E01-GAL4 in driving the expression of either was comparable as shown by the similar GFP levels (Figures 2AC, I, and J).
In mammals, the hallmarks of astrocyte activation are hypertrophy of astrocytes and upregulated level of the glial fibrillary acidic protein (GFAP) (Pekny et al., 2004).
Given that there's no GFAP homolog in Drosophila (Doherty et al., 2009), we examined morphological changes in astrocytes at single-cell resolution following TBI by mosaic analysis with a repressible cell marker (MARCM) on adult fly brains at 3-4 days after eclosion.The cell bodies of these astrocytes were located at the interface between neuropil and cortex regions, or in neuropils, and their cellular processes infiltrated into neuropils (Figure 2E).Interestingly, astrocytes in injured brains exhibited bright and distinct GFP + accumulations associated with their cellular processes at 24 h after TBI (Figures 2F and 2G).Quantitatively, the number of these accumulations per square nanometer in the cellular processes was significantly increased at 24 h after TBI; the increase in the number of accumulations might be responsible for the increase in GFP intensity triggered by TBI (Figure 2G).
Importantly, the diameter of astrocytes cell bodies was also increased at 24 h after injury (Figure 2H).Taken together, these results show that astrocytes become reactive in adult brains at 24 h after TBI.
Axotomy induces expression of injury-related proteins such as extracellular proteases the matrix metalloprotease (MMP) (Purice et al., 2017).In addition to the morphological changes in astrocytes, we observed upregulated expression of Mmp1 in TBI-treated brains (Figures 2IJ'', 2MN'', and 2Q).These Mmp1 + signals were colocalized with membrane-bound mCD8::GFP driven by the astrocytes-specific driver R86E01-GAL4 or ENG-specific driver R56F03-GAL4 (Figures 2K, 2L, 2O, and 2P), with a more dominant expression in ENG.These results suggest that Mmp1 might be produced from both types of glia after TBI.

Transcriptional profiling of astrocytes in adult flies reveals elevated expression of injury-related genes upon TBI
To further investigate how astrocytes respond to TBI, we analyzed transcriptional changes in astrocytes by RNA-seq (Figure 3A).Due to the tiny soma and morphological complexity, the analysis of a purified population of glial cells has been difficult and technically challenging.We have optimized a protocol for purifying astrocytes from adult Drosophila of R86E01-Gal4>UAS-Redstinger by fluorescence-activated cell sorting (FACS) (see details in Materials and Methods).As FACS removes most cell masses and RFP-negative cells, the remaining RFP + cells are mostly viable, as shown by MitoTracker staining, and purity was 92.96% (RFP + and MitoTracker + cells/total cells) in uninjured brains (Figures S1A-C').Further analysis of the RNA-seq data showed that the astrocyte markers Eaat1 (110359 reads) and alrm (186413 reads) were highly expressed, while nSyb, a neuronal marker, was hardly detected (125 reads) at 4 h after TBI, verifying the purity of FACS-sorted astrocytes.A principal component analysis (PCA) revealed that the variance between different groups (no injury vs. 4 h and 24 h after injury) was greater than the variance within groups (Figure S1D).From analysis on five biological replicates for each time point (no injury, 4 h, and 24 h after injury), we obtained 539 million reads (an average of 36 million reads per sample) and aligned the RNA-seq reads to the Drosophila genome.We found that 93.67 ± 0.89% of reads mapped to the Drosophila genome (dm6 version).Using a cutoff of ≥ 2-fold differential expression (adj.p value <0.01, Benjamini-Hochberg procedure), we identified 522 and 357 genes with altered expression at 4 h (416 upregulated and 106 downregulated) and 24 h (229 upregulated and 128 downregulated) after injury, respectively (Figures 3BD; Table S2).
Gene Ontology (GO) analysis of these upregulated genes revealed that a significant proportion were associated with endosomal transport, actin cytoskeleton, and wound healing (Figure S2).Furthermore, we detected upregulated expression of components functioning in the innate immune response after TBI.For instance, expression of the components Relish and Dif of the Toll and Imd pathways was upregulated in astrocytes after TBI (Figure 3D), in agreement with a previous finding that Relish is necessary for TBI-induced secondary injuries (Swanson et al., 2020).In addition, expression of injury-related genes including chic, Jra, kay, rl, Cad96Ca, Idgf4, Stam, draper, Pvr, spict, Mmp1 and NijA, and receptor genes including Pvr, draper, AdoR, PGRP-SA, and NimA were upregulated in astrocytes at 4 h or 24 h after injury (Table S3).A set of endocytic trafficking genes was also identified, including Arpc1, Arpc2, cpa, cpb, Stam, and Chmp1.Subsequent RT-qPCR experiments confirmed the upregulation of all six genes tested at 4 h and/or 24 h after TBI (Figure 3E), supporting the robustness of the RNA-seq analysis.As a control, the astrocytes-specific alrm gene showed no upregulation upon TBI by RT-qPCR and RNA-seq.Taken together, these findings validate the approach of RNA-seq transcription profiling in detecting genes functionally related to injury responses in astrocytes following TBI.

Pvr and AP-1 are required for the upregulation of Mmp1 after TBI
Among the candidates exhibiting upregulated expression in astrocytes upon TBI, three receptors (Pvr, AdoR and Draper), all acting upstream of and activating the JNK pathway (Ishimaru et al., 2004;Macdonald et al., 2013;Poernbacher and Vincent, 2018), emerged as new factors with uncharacterized functions in astrocytes (Figure 3D).The Drosophila RNA interference (RNAi) Screening Center Integrative Ortholog Prediction Tool (DIOPT) identified orthologs of these three receptors, subunits of transcriptional factor AP-1 (composed of Jra and kay) of the JNK pathway, Mmp1, and endocytic trafficking genes in mouse and human (Table 1) (Hu et al., 2011).These proteins mediate essential injury responses in vertebrates (Borea et al., 2016;Motz and Coukos, 2013;Shi et al., 2021).To examine a potential role of the receptors and their downstream signaling components in astrocytes after TBI, we quantified the immunostaining intensity of these proteins from projected images of the whole brain (including 20-30 slices with 1 µm per slice).As our RNA-seq analysis revealed elevated expression of JNK-related injury genes such as AP-1 and Mmp1 (Figure 3D and Table 1), we analyzed the expression of Mmp1, a transcriptional target of AP-1 (Purice et al., 2017), as a readout for the activity of JNK signaling in response to TBI.We found that upregulated Mmp1 expression induced by TBI was potently suppressed by knocking down the expression of Pvr, Jra or kay (Figures 4AD, 4F), indicating that Mmp1 upregulation is mediated by Pvr and AP-1, and predominantly occurrs in astrocytes.We note that the location and intensity of Mmp1 signals are variable among different individuals (Figure 4), probably because the brain damage caused by HIT is not stereotypical.
Even though flies with Draper knockdown in astrocytes exhibited high mortality, analysis of escapers indicated that Mmp1 upregulation upon TBI was not completely inhibited by Draper knockdown (Figure 4E and 4F).Knockdown of the other receptor AdoR in astrocytes caused a weak inhibition of AP-1 and Mmp1 upregulation (Figure S3A-A'', S4G-H'', S4I and S4J); TRE-dsRed that expresses the red fluorescent protein dsRed under the control of 4  AP-1 motifs (Chatterjee and Bohmann, 2012) was used as a reporter for AP-1 activity.As Pvr showed the strongest regulation on Mmp1 expression among the three receptors (Figure 4 and S3), we focused on the potential role of Pvr in astrocytes following TBI in the present study.It is noteworthy to mention that although Draper expression was upregulated upon axotomy (MacDonald et al., 2006) and TBI (Figure 3D), inhibiting Draper expression in astrocytes did not suppress the Mmp1 upregulation after TBI (Figure 4E and 4F).Taken together, these results suggest that Pvr, but not Draper, and AP-1 are required for upregulated Mmp1 in astrocytes after TBI.

TBI
We next investigated the genetic pathway by which Pvr functions in astrocytes in response to TBI.We examined the activity of AP-1 using the in vivo reporter TRE-dsRed (Chatterjee and Bohmann, 2012).In uninjured brains, we detected low TRE-dsRed levels, suggesting a basal AP-1 activity (Figure 5A-A'').Similar to Mmp1, TRE-dsRed levels were robustly upregulated at 24 h after TBI, suggesting activation of JNK/AP-1 signaling (Figure 5B-B'').Importantly, TBI-induced expression of TRE-dsRed and Mmp1 was suppressed or further upregulated when inhibiting or overexpressing Pvr expression in astrocytes, respectively (Figure 5C  F'').
Overexpression of Jra or kay alone did not affect expression of TRE-dsRed or Mmp1 (Figure S3A-F'', 5I and 5J), suggesting that overexpressing a single subunit of the AP-1 complex is not sufficient to upregulate AP-1 activity.While Pvr overexpression caused an increase in TRE-dsRed and Mmp1 expression levels, Jra or kay RNAi knockdown in the background of Pvr overexpression suppressed the increase (Figure 5G-L), indicating that Jra and kay act downstream of Pvr to mediate the injury response upon TBI.These results together show that Pvr is a receptor acting upstream of AP-1 signaling that promotes Mmp1 expression during the injury response in astrocytes (Figure 5M).

Mmp1 upregulation upon TBI
How was the Pvr signalling induced by TBI regulated?It has been shown that VEGFR2 endocytosis proceeds in a clathrin-dependent manner (Simons et al., 2016).
VEGFRs are transmembrane RTKs that are activated when they locate at the cell membrane (Simons et al., 2016).We first investigated whether endocytosis is involved in Pvr signaling upon TBI.Shibire (shi) encodes a GTPase called dynamin which is responsible for fission of the vesicle from the membrane (van der Bliek and Meyerowitz, 1991).We analysed the effect of endocytosis on Pvr signaling by blocking the activity of dynamin with a temperature sensitive mutant, shi ts , in astrocytes.Interestingly, both Pvr (Figures 6A, 6B, and 6E) and TRE-dsRed (Figures 6C, 6D, and 6F) intensities were increased at 4 h after TBI when dynamin activity was blocked at the non-permissive 32°C.As a control, expression of nLacZ under the same conditions as mutated dynamin did not exhibit a significant difference in the intensities of Pvr and TRE-dsRed at the permissive 23°C and the non-permissive 32°C after TBI (Figures 6A, 6B, 6E, and 6F).These results suggest that Pvr signaling is facilitated when endocytosis is blocked.
In addition to Pvr, results from our transcription profiling analysis also revealed upregulation of the expression of actin dynamics and endocytic trafficking genes including Arpc2, cpa, and cpb following TBI (Tables 1 and S2).To explore whether endocytic trafficking genes are involved in the injury response in astrocytes, we analyzed Mmp1 and TRE-dsRed expression when inhibiting the expression of Arpc2, cpa, or cpb.Interestingly, TBI-induced Mmp1 levels were further increased after knockdown of each of the three (Figures 7A-D, 7O); consistently, TRE-dsRed expression was also increased after knockdown of cpa or cpb (Figures 7H-K, 7P).
These results indicate that genes involved in endocytosis and endocytic trafficking normally inhibit induction of AP-1 and Mmp1 by TBI in astrocytes.
In addition, we also observed upregulated expression of a set of endosomal sorting genes, including Stam, Chmp1, and Vps24 that encode components of the endosomal sorting complex required for transport (ESCRT)in astrocytes in response to TBI (Tables 1 and S2).These genes are implicated in the formation of intraluminal vesicles (ILVs) from multivesicular bodies (MVBs).Inhibiting expression of the ESCRT-0 subunit Stam and the ESCRT-III subunits Chmp1 or Vps24 in astrocytes however did not result in a significant difference in Mmp1 or TRE-dsRed expression levels compared with controls following TBI (Figure 7E-G, 7L-N, 7O and 7P), suggesting that endosome sorting in MVB may not affect Pvr signaling activity.

Discussion
Using a previously established a Drosophila TBI model, we found that TBI causes neuronal apoptosis, BBB/BEB disruption, and astrocyte swelling.Astrocytes respond to TBI by elevated Pvr expression, which in turn enhances the expression of the downstream effectors AP-1 and Mmp1.Our findings identify a new signaling pathway upregulated in astrocytes following TBI.
Unlike vertebrates, in which astrocytes become reactive (reactive astrogliosis) and actively clear neuronal and synaptic debris via phagocytosis upon axon injury (Liddelow and Barres, 2017), axon transection in Drosophila shows no immediate astrocytic response to injury (Doherty et al., 2009;Purice et al., 2017).Instead, Drosophila ENG express key components of the phagocytic machinery and engulf neuronal debris in response to axotomy (Doherty et al., 2009).Here, we examined for the molecular and cellular changes of astrocytes after TBI.Upon TBI, astrocytes exhibited apparent damage response including enlarged soma and altered cellular processes containing more membrane-bound GFP + accumulations (Figure 2A  J).
These findings suggest that astrocytes are activated upon TBI, demonstrating a critical role for astrocytes in the injury response.
The innate immune response in Drosophila relies on the activation of multiple signaling pathways including NF-κB/Relish-mediated Imd and Doral/Dif-mediated Toll signaling pathways.Interestingly, our RNA-seq data revealed elevated expression of genes Relish and Dif in both pathways upon TBI (Figure 3D).However, the expressions of AMPs were minimum in astrocytes before TBI and no AMPs genes were identified in differentially expressed genes.Thus, AMPs genes were not activated in astrocytes, though upregulated AMP expressions were detected by bulk RNA-seq after TBI.In addition, expression of AP-1 (composed of Jra and kay) and Mmp1 was also upregulated, indicating that the AP-1signaling pathway is activated in astrocytes following TBI.Even though upregulated Mmp expression in reactive glial responses is an evolutionarily conserved feature of Drosophila and mammalian glia (Cunningham et al., 2005;Purice et al., 2017), transmembrane receptors on astrocytes that regulate Mmp1 expression have not been identified.In mammals, knockdown of VEGF signaling in keratinocytes reduced angiogenesis and delayed wound healing (Rossiter et al., 2004).In Drosophila, studies of wound healing showed that Pvr likely acts upstream of actin regulatory proteins that promote actin polymerization to lead cells migration (Brock et al., 2012;Wu et al., 2009).
Through a combination of RNA-seq and genetic analyses, we identified Pvr as a new receptor in astrocytes; Pvr promotes Mmp1 expression via the transcription factor complex AP-1.At present, we do not know how Pvr promotes expression of AP-1 and Mmp1.But previous findings show that Pvr phosphorylates JNK to trigger the downstream signaling containing AP-1 during thorax closure in metamorphosis (Ishimaru et al., 2004).During development, the midline glia survival and migration are regulated by the ventral unpaired median (VUM) and the midline precursor 1 (MP1) midline neurons, the sources of Pvr ligands, i.e., PDGF-and VEGF-related factor 1-3 (Pvf1-3) (Learte et al., 2008).Additionally, epidermal cell-derived Pvf1 may be sequestered in the blood and exposed upon injury to bind Pvr receptors on wound-edge epidermal cells and initiate the extension of cell processes into the wound gap (Wu et al., 2009).Specifically, Pvr ligands bind to Pvr receptor to induce autophosphorylation of tyrosine residues, leading to JNK phosphorylation.Phosphorylated JNK (Bsk) then phosphorylates Jun/Jra transcription factors, triggering Jun/Jra association with Fos/kay, to form the AP-1 complex (Bogoyevitch and Kobe, 2006).In addition, glial AP-1 is essential for recovery, ensuring brain integrity and animal survival in the early post-TBI period (Byrns et al., 2021).Given that Pvr and AP-1 genetically interact in astrocytes upon TBI, it is likely that Pvr acts upstream of AP-1 and regulates its activity via JNK phosphorylation following brain injury.We speculate that Pvr ligands that may secret from injured cells to activate Pvr-positive astrocytes after TBI; activated Pvr then increases Mmp1 expression via AP-1 and secretion to the intercellular space to participate in injury response.
In addition to Pvr, our RNA-seq data also identified other receptors potentially functioning in astrocytes in response to TBI.Specifically, AdoR expression was upregulated after TBI (Table S2), and knockdown of AdoR expression in astrocytes led to inhibition of AP-1 after TBI (Figure S3G-J), though at a weaker extent compared with Pvr knockdown.In mammals, A2BR, a subtype of AdoR, was shown to stimulate VEGF production in various cell types (Borea et al., 2016).Pvr promotes the expression of adenosine deaminase-related growth factor A (Adgf-A) which inactivates extracellular adenosine, the ligand of AdoR (Zurovec et al., 2002).
Furthermore, Pvr and AdoR collaborate in differentiating cells to maintain the expression of Adgf-A (Mondal et al., 2011).Thus, it is possible that Pvr and AdoR act independently or in a coordinated manner to regulate the injury response to TBI in astrocytes in Drosophila.
Our RNA-seq profiling also revealed upregulation of Draper.Previous studies revealed a role for Draper-mediated phagocytosis of axonal debris in ENG after axotomy (Lu et al., 2017;MacDonald et al., 2006); axotomy induces Wallerian-like degeneration (Büki and Povlishock, 2006;Tang-Schomer et al., 2010).Specifically, damage-associated molecular pattern (DAMP) proteins released from injured neurons bind to Draper and activate the JNK and JAK/STAT pathways in ENG (Doherty et al., 2014;Freeman, 2015;MacDonald et al. 2013).Here, our findings showed that Draper does not affect Mmp1 expression in astrocytes upon TBI.Although an increase in Draper expression was detected in astrocytes by RNA-seq, knockdown of its expression did not significantly suppress Mmp1 upregulation after TBI.Thus, Draper activates JNK/AP-1 and JAK/STAT signaling in ENG in response to axotomy (Doherty et al., 2014;MacDonald et al. 2013), whereas astrocytes respond to TBI via Pvr-mediated AP-1 signaling.Despite different pathways are activated in different types of glia upon brain injury, they may cooperate in response to TBI.
Endocytosis and trafficking of VEGFR, the mammalian Pvr homolog, regulate the specificity as well as the duration and strength of the signaling output (Simons et al., 2016).Once activated on the plasma membrane and translocated into the cytoplasm, VEGFRs are either shuttled to lysosomes for degradation or recycled back to the plasm membrane to replenish the receptor pool (Simons et al., 2016).Our findings showed that disruption of endocytosis by disrupting dynamin activity and inhibiting the expression of Arpc2, cpa, or cpb that regulate endocytic transport into early endosomes enhanced the AP-1 and Mmp1 upregulation in astrocyte following TBI (Figures 7A-D, 7H-K, 7O, 7P).As receptor endocytosis attenuates the strength or duration of many signaling processes by physically reducing the concentration of cell surface receptors accessible to the ligand (Jékely et al., 2005), blocking dynamin-mediated endocytosis and endocytic trafficking potentially results in accumulated Pvr receptors on the plasma membrane to activate the downstream effector AP-1, thereby enhancing the astrocytes response to TBI.On the other hand, ubiquitinated RTKs are recognized by ESCRT-0, followed by sequential recruitment of ESCRT-I, ESCRT-II, and ESCRT-III onto the membranes of MVBs to form intraluminal vesicles (ILVs) (Goh and Sorkin, 2013).Knockdown of the ESCRT-0 subunit Stam and the ESCRT-III subunits Chmp1 and Vps24 did not affect the expression of Pvr downstream signaling components (Figure 7E-G, 7L-N, 7O and 7P), suggesting that Pvr signal transduction was not regulated by endosomal sorting.
Considering the importance of VEGF signaling in a variety of cellular processes, including wound repair (Folkman and Klagsbrun, 1987), elucidating the downstream signaling mechanisms, as well as defining the full array of Pvr targets in healthy and diseased brains, will be critical for dissecting the protective or detrimental roles of VEGF in the context of brain injury.Given that the Pvr receptor, AP-1, and the MMP family of secreted proteases are all highly conserved across species, our work provides new insights into how VEGF signaling in glia mechanistically contributes to the TBI response in mammals.

Figure 1 .
Figure 1.TBI is induced in adult flies by a high impact trauma (HIT) device.(A) MI24 is the mortality index calculated from the percentage of dead over total flies.Increasing the number of strikes from 4 to 6 increases the MI24 value.Error bars denote standard error of the mean (SEM; ***p <0.001, Student's t-test).(B-E) The intactness of blood-eye-barrier (BEB) and blood-brain-barrier (BBB) before or after 6 strikes is indicated by the accumulated fluorescence intensity at the border of the eye (B, D) and brain (C, E), respectively.The examination was carried out at 2 h after fluorescent dye injection.Arrows indicate the hemolymph exclusion line.Scale bar = 100 μm.(F) The percentage of permeable BEB with or without TBI (***p <0.001).(G) The percentage of permeable BBB following different numbers of strikes.Error bars denote SEM (**p <0.01 and ***p <0.001, Student's t-test).(H) Histogram showing mRNA expression levels of innate immune response genes of the AMP family (Attc, DiptB and Mtk) in the adult brain.Expression was increased in all cases following treatment.The RPL28 gene served as an internal reference.Results are means ± SEM (***p <0.001, Student's t-test).(I, J) The number of Dcp-1 + puncta (green) increased in injured adult brains 24 h after TBI.Scale bar = 50 μm.(K) Quantification of Dcp-1 + puncta.Error bars denote SEM (**p <0.01, Student's t-tests).Results are means ± SEM. (L-M'') Apoptotic debris labelled with anti-Dcp-1 antibodies (Red).Glial and neuronal nuclei are labelled with anti-Repo (L) and anti-Elav (M) antibodies (Blue), respectively.Scale bar = 20 μm.

Figure 2 .
Figure 2. TBI alters glial morphology and upregulates Mmp1 expression.(A-C) Representative images of astrocyte membranes labelled with mCD8::GFP driven by the astrocyte driver R86E01-Gal4.Co-expression of UAS-Redstinger labels astrocyte nuclei.GFP intensities are increased at 4 h (B) and 24 h (C) after injury.The rectangle defined by white dash lines denotes antennal lobes.Scale bars = 100 μm.(D) Quantification of GFP intensity at different timepoints after TBI.Results are

Figure 3 .
Figure 3. Transcriptome analysis of astrocytes reveals upregulation of injury-related and innate immunity genes.(A) After dissection, Drosophila brains were disrupted and the cell suspension was subjected to FACS.Astrocyte cDNAs were amplified by the Smart-seq2 method and a cDNA library was constructed.A subset of upregulated genes from RNA-seq screening was validated by quantitative real-time PCR.(B, C) Volcano plots of the

Figure 5 .
Figure 5. Pvr is required for AP-1 upregulation after TBI.(AB'') Adult brains showing increased TRE-dsRed (an AP-1 reporter) expression at 24 h after TBI.(C  D'') Overexpression of Pvr in astrocytes leads to upregulated dsREd expression compared with control flies.Scale bar = 100 μm.(E  F'') Knockdown of Pvr in astrocytes causes downregulation of dsRed expression compared with control flies after TBI.(GH'') Overexpression of Pvr and knockdown of Jra at the same time in astrocytes causes downregulation of dsRed and Mmp1 after

Figure 7 .
Figure 7. Knockdown of endocytic trafficking-related genes up-regulates expression of AP-1 and Mmp1upon TBI (A, H) Representative Mmp1 immunostaining (magenta) and dsRed intensity (magenta) in control animals (R86E01-Gal4) at 24 h after TBI.(B-D, I-K) Knockdown of Arpc2, cpa, and cpb in astrocytes results in further upregulation of Mmp1 and dsRed upon TBI.All images are projections of 25-30 m immunostaining slices (one image per 1 m slice).(E-G, L-N) Knockdown of Stam, Chmp1 or Vps24, components of ESCRT, in astrocytes had no influence on Mmp1 and dsRed upregulation compared with control after TBI (A, H).All images are projections of 25-30 m immunostaining slices (one image per 1 m slice).(O, P) Quantification of Figure 2 Figure 4No injury E R86E01>draper RNAi Figure 5