Removal of developmentally regulated microexons has a minimal impact on larval 1 zebrafish brain morphology and function

Abstract

2014; Quesnel-Vallières et al., 2015).Srrm4 interacts with Srsf11 and Rnps1 at polypyrimidine sequences upstream of microexons to regulate their inclusion (Gonatopoulos-Pournatzis et al., 2018).Proteins that regulate and contain microexons are involved in neurodevelopmental disorders.In the brains of individuals with autism spectrum disorder (ASD), the level of SRRM4 is reduced, leading to the misregulated splicing of multiple microexons (Irimia et al., 2014).Mice haploinsufficient for Srrm4 display reduced social interaction, sensory hypersensitivity, and impaired synaptic transmission (Quesnel-Vallières et al., 2015).Furthermore, Srrm4 is involved in regulating the ASD-relevant KCC2-dependent GABAergic excitatory to inhibitory switch (Nakano et al., 2019), and a mouse model of ASD with disrupted Pten has decreased Srrm4 expression and microexon inclusion (Thacker et al., 2020).Loss-of-function variants in its interacting partner SRSF11 have been implicated in ASD in humans (C Yuen et al., 2017).While the behavioral roles of only a few microexons have been studied in detail (Gonatopoulos-Pournatzis and Blencowe, 2020), the elimination of one from Eif4g1 altered synaptic plasticity and social behavior in mice (Gonatopoulos-Pournatzis et al., 2020).Many genes containing microexons also lead to neurodevelopmental disorders when mutated in humans.Examples include CASK-mediated microcephaly and cerebellar hypoplasia, SPTAN1-mediated childhoodonset epilepsy, and MAPK8IP3-mediated intellectual disability (Giacomini et al., 2021;Platzer et al., 2019;Syrbe et al., 2017).Taken together, this splicing program, the genes with microexons, and microexons themselves are important to proper neural development.Although most microexons are unstudied, detailed structural and functional characterization of a small subset has revealed involvement in synapse development.The most well-studied are found in the receptor-type tyrosine phosphatase (e.g., ptprf) and teneurin (e.g., tenm4) gene families.Recognized in the 1990s (O'Grady et al., 1994;Pulido et al., 1995), two microexons in presynaptic PTPδ (Ptprd), PTPσ (Ptprs), and LAR (Ptprf) modulate trans-synaptic interfaces and determine selective interaction with postsynaptic partners (Y.Li et al., 2015;Lin et al., 2018;Park et al., 2020;Um et al., 2014;Yamagata et al., 2015;Yim et al., 2013).Similarly, differential inclusion of two conserved microexons in teneurins can modulate trans-synaptic homophilic or heterophilic interactions through splicing-dependent, large conformational changes (Berns et al., 2018;Gogou et al., 2024;Li et al., 2020).While studying these cell adhesion molecules has highlighted how microexons can trigger differential protein-protein interactions, little is known about the developmental consequences of these biochemical switches in other protein classes.Identifying the neurobiological roles of many individual microexons is a challenge.A vertebrate model is necessary for these studies; although there is a similar splicing mechanism in Drosophila melanogaster, there is no overlap of the individual microexons (Torres-Mendez et al., 2021).Thus, we used larval zebrafish, a vertebrate that supports more high-throughput studies than mammalian models (Thyme et al., 2019), to assess the brain activity, brain structure, and behavior of zebrafish larvae mutant for 45 microexons (Figure 1A).

Results
A recently developed computational tool identified microexons that are differentially spliced during mouse brain development and conserved in zebrafish (Parada et al., 2021).Based on the intersection of these two sets, 95 microexons fulfilled both parameters (Table S1).Of these 95 microexons, 44 exist in a canonical layout in the zebrafish genome, with a TGC and UC repeats for microexon-containing regions over zebrafish development.These data were clustered using the default seaborn clustermap settings (method = 'average').Mutants'''''''''''''''Siblings'   S2.
Kruskal-Wallis ANOVA p-values for the selected plots are as follows: dop1a center preference during the first night (day0night_boutcenterfraction_3600) = 0.00006/0.0008;eif4g3b p-values are not calculated for response plots (shown is all dark flashes in block 1), p-values for the latency for the first 10 dark flashes in block 1 (day6dpfdf1a_responselatency) = 0.026/0.0008,;ppp6r3 frequency of response to strong acoustic stimuli with a sound frequency of 1000 hertz that precede the habituation block (day5dpfhab1pre_responsefrequency_1_a1f1000d5p) = 0.002/0.016;ptprd-1 pixels moved in each bout for the duration of the experiment (combo_boutcumulativemovement_3600) = 0.001/0.00002;rapgef2 number of bouts for the duration of the experiment calculated using the delta pixel data in each frame (dpix_numberofbouts_3600) = 0.002/0.001.directly upstream of the alternatively-spliced exon (Gonatopoulos-Pournatzis et al., 2018) (Table S1), indicating that their inclusion is likely controlled by Srrm4.Of the remaining microexons, most are similar to the canonical layout and are likely similarly regulated.The protein sequences of the 95 microexons are highly conserved between mouse and zebrafish (Figure 1B), more so than the full-length protein sequences (Figure 1C).The genes containing microexons are enriched for those involved in neuronal development as well as the cytoskeleton (Figure 1D).The majority of these microexons are included in transcripts as the brain develops, rather than lost, in both mouse and zebrafish (Figure 1E), making CRISPR/Cas9 removal of the microexon an ideal approach for studying the function of the added amino acids.We generated 45 CRISPR/Cas9 lines that removed conserved microexons (Table S2) and assayed larval brain activity, brain structure, and behavior (Figure 1A).Protein-truncating mutations in additional genes that contain microexons revealed developmental and neural phenotypes in zebrafish (Figure S1, Figure S2), indicating the genes themselves are involved in biologically relevant pathways.Although we were most interested in those microexons only included after 24 hours post-fertilization (Figure 1E), we also removed microexons from several genes with an earlier expression of the isoform.
To uncover neural phenotypes, we assessed baseline and stimulus-driven movement.Homozygous mutant and control sibling larvae were subjected to a behavioral pipeline in 96well plates from 4-7 days post-fertilization (dpf) (Figure 2A).Only a small number of strong, repeatable phenotypes were observed either at baseline or in response to acoustic or visual stimuli (Figure 2B, Figure 2C, Figure S3, Figure S4, Figure S5).Baseline behavioral differences were observed in multiple movement categories.For example, rapgef2 mutants showed an increased movement frequency (Figure 2B, Figure 2D) but no changes to the characteristics of these movements (magnitude) or location in the well.In contrast, dop1a mutants had reduced daytime movement and an increased well-edge preference specifically at night (Figure S4, Figure S6, Figure 2B, Figure 2D).Other baseline movement phenotypes included a well-center preference for vav2 mutants and a decreased movement magnitude for ptprd-2 (also referred to in the literature as meA) mutants (Figure S6).Dark-flash response phenotypes included a reduced latency for eif4g3b mutants and a reduced response frequency for ptprd-1 (meB) and dop1a mutants (Figure 2C, Figure 2D, Figure S6).Acoustic response phenotypes included an increased frequency of response to strong stimuli for ppp6r3 mutants and a reduced frequency of response to strong acoustic stimuli for ptprd-2.To detect changes to brain development and function, we collected phospho-Erk (pErk) brain activity maps under unstimulated conditions at 6 dpf.Large morphological differences were identified using the Jacobian matrix derived from the image registration process (Jefferis et al., 2007;Rohlfing and Maurer, 2003).Similar to the behavioral findings, few phenotypes were observed in brain activity (Figure 3A, Figure S7, Figure S8) or brain structure (Figure 3B, Figure S9, Figure S10) in homozygous mutants compared to wild-type control siblings when quantified by region (Figure 3C).Repeatable phenotypes were mild mild (Figure 3D) compared to a typical protein-truncating mutant (see control-tcf7l2).Only one mutant, ppp6r3, displayed substantial and repeatable differences in brain morphology (Figure 3B, Figure 3E), with reduced size observed mainly in the thalamus and neighboring pretectum.While the loss of both copies of microexons was required for most phenotypes, brain activity differences were observed in vti1a and sptan1 heterozygotes (Figure 3F) as well as vav2 and mapk8ip3 (Figure S7, Figure S8).

Discussion
Our study provides a broad overview of the larval zebrafish neural phenotypes resulting from removing developmentally regulated microexons.Here, we leveraged our previously successful brain activity mapping and behavioral profiling strategy, which has high sensitivity and has uncovered phenotypes for dozens of mutants (Thyme et al., 2019).While only a few zebrafish lines had neural phenotypes, most removed microexons with behavioral or brain activity differences were completely unstudied previously and found in diverse protein classes.The small number of observed phenotypes was unexpected based on conservation of the microexons (Figure 1B, Figure 1C) and the discovery rates of previous screens using similar methods (Capps et al., 2024;Thyme et al., 2019;Weinschutz Mendes et al., 2023).Although most of these studies focused on protein-truncating alleles, we have found phenotypes in lines with missense mutations (Capps et al., 2024).Furthermore, the genes that contain microexons are developmentally important (Figure S1).For instance, the caska loss-of-function mutant displayed substantial changes to brain activity, a smaller brain size, and multiple behavioral phenotypes.The low number and expressivity of microexon-specific phenotypes raises the question of whether perturbation of multiple microexons simultaneously, as was observed in the brains of autistic individuals (Irimia et al., 2014), would have a more pronounced effect.While zebrafish homozygous for srrm4 deletion did not display any overt developmental phenotypes (Ciampi et al., 2022), a detailed neural characterization and behavior was not completed and this result is in contrast to a previous morpholino study (Calarco et al., 2009).Alternatively, although the microexons are present at larval stages, they may only become important during later stages of neuronal maturation that support more complex behaviors.Mouse models were studied as adults and for disruptions to social interaction, learning, and memory (Gonatopoulos-Pournatzis et al., 2020;Han et al., 2024), which larvae are not yet capable of (Dreosti et al., 2015;Valente et al., 2012).
Our study extends on findings for previously studied genes containing microexons.We discovered increased brain activity (Figure 3D) and an altered dark flash response in mutants for the eif4g3b (Figure 4D), whereas a mouse model with the microexon removed was described as having no behavior phenotypes (Gonatopoulos-Pournatzis et al., 2020).That study, however, focused on social behavior, learning, and memory and may not have included stimuli analogous to dark flashes.Alternatively, microexon removal in zebrafish could have differential behavioral impacts than in rodents.Mice mutant for the ptprd-2 (meA) microexon have increased movement and interrupted sleep (Park et al., 2020).Both zebrafish mutants in ptprd demonstrate the opposite, with reduced daytime movement and increased sleep during the day for ptprd-2 (Figure S6).While the underlying disrupted circuitry could differ between the species, the daytime sleep increase raises the possibility of a rebound response to undetected disruptions to nighttime sleep.In other cases, the gene that contains the microexon has been extensively studied, but research on the microexon itself is absent.For example, the function of SNARE protein Vti1a in neural development and dense core vesicle biogenesis has been studied in great depth (Bollmann et al., 2022;Emperador-Melero et al., 2018;Kunwar et al., 2011;Sokpor et al., 2021;Walter et al., 2014).Its brain-specific microexon, however, was recognized over twenty years ago (Antonin et al., 2000), and its specific importance has been largely ignored.We discovered reduced brain activity mainly in the telencephalon in both homozygous and heterozygous lines (Figure 3F), highlighting the relevance of considering multiple conserved isoforms when characterizing protein function in the brain.
Mutants for unstudied microexons with the strongest phenotypes come from diverse protein classes (Gonatopoulos-Pournatzis and Blencowe, 2020), indicating this splicing program impacts neurodevelopmental processes beyond trans-synaptic partner selection.The only mutant with a substantial brain size difference was ppp6r3, which encodes a regulator of protein phosphatase 6 (PP6).Although little is known about this protein, it has been linked to cell cycle (Heo et al., 2020) and cell death (Wu et al., 2022), nominating hypotheses for determining future research on the source of the reduced size.Dctn4 is part of the dynactin complex, Mapk8ip3 is involved in Kinesin-1-Dependent axonal trafficking (Platzer et al., 2019;Watt et al., 2015), and Sptan1 is a cytoskeleton scaffold protein (Huang et al., 2017); the number microexon-containing genes related to the cytoskeleton (Figure 1D) and also trafficking (e.g., vti1a), suggests that microexons may be important to the unique cytoskeleton needs of developing neurons.The functions of microexons in cytoskeletal proteins are beginning to be discovered (Poliński et al., 2023).Transcriptional regulation is represented by the meaf6 mutant, while Rapgef2, which has the strongest behavioral phenotypes upon microexon removal (Figure 3D), is a signaling protein.
Although our list is not comprehensive, as many microexons remain unstudied, from 45 tested, we have prioritized approximately ten for future study.As microexons are often found in surface-accessible protein domains and can regulate protein-protein interactions (Dergai et al., 2010;Irimia et al., 2014), proximity-dependent ligation in zebrafish lines with and without the microexon could reveal differential binding partners in vivo (Rosenthal et al., 2021).The discovery of microexon mutants with neural phenotypes lays the groundwork for future investigation of impacted neurodevelopmental pathways and how interactomes differ between isoforms.

Zebrafish husbandry
The UAB Institutional Animal Care and Use Committee approved all zebrafish experiments (IACUC protocol numbers 22155 and 21744).Mutants were generated in an Ekkwill-based strain using CRISPR/Cas9 as previously described (Capps et al., 2024;Thyme et al., 2019) (Table S2).Both adults and larvae were maintained on a 14 h/10 h light/dark cycle at 28°C.Experimental larvae were maintained at a density of less than 160 per dish in 150 mm petri dishes in fish water with methylene blue, and debris was removed at least twice prior to experimentation.Visibly unhealthy larvae and those without inflated swim bladders were excluded from experiments.Control animals were always siblings from the same clutch and derived from a single parental pair, and all larvae were genotyped after experimentation.The N for each assay is available in Table S2.
Brain activity and morphology Phosphorylated-ERK (pErk) antibody staining was conducted as previously described (Capps et al., 2024;Thyme et al., 2019).The total ERK antibody (Cell Signaling, #4696) was used at 3:1000 and pErk antibody was used at 1:500 (Cell Signaling, #4370).Typically, the primary antibody exposure was for 2-3 days and secondary for one day.Image stacks were collected with a Zeiss LSM 900 upright confocal microscope using a 20X/1.0NA water-dipping objective.These stacks were registered to the standard zebrafish Z-Brain reference using Computational Morphometry Toolkit (CMTK) (Jefferis et al., 2007;Randlett et al., 2015;Rohlfing and Maurer, 2003;Thyme et al., 2019).The significance threshold for MapMAPPING (Randlett et al., 2015;Thyme et al., 2019) was set based on a false discovery rate (FDR) where 0.05% of control pixels would be called as significant for both the brain activity and structural measurements.Larval behavior Larval behavior assays were conducted and analyzed as previously described (Capps et al., 2024;Joo et al., 2020;Thyme et al., 2019).The pipeline begins on the evening of larval stage 4 dpf and includes the following stimuli: 5 dpf light flashes (9:11-9:25), mixed acoustic stimuli (prepulse, strong, and weak, from 9:38-2:59), and three blocks of acoustic habituation (3:35-6:35); the night between 5 and 6 dpf mixed acoustic stimuli (1:02-5:00) and light flashes (6:01-6:20); 6 dpf three blocks of dark flashes with an hour between them (10:00-3:00) and mixed acoustic stimuli and light flashes (4:02-6:00).Stimulus responses were quantified from high-speed (285 frames-persecond) 1-second-long movies.All code for behavior analysis is available at https://github.com/thymelab/ZebrafishBehavior.Frequency of movement, magnitude of movement, and location preferences were calculated for the baseline data.An example of a "frequency" measure would be the active seconds / hour.An example of the "magnitude" measure would be the movement velocity of a bout.An example of a "location" measure would be the fraction of the bout time spent in the center of the well.Frequency, response magnitude parameters, and latency to response are also calculated for each stimulus response from the highspeed movies.The stimulus responses are also broken up into subsets, such as the dark flashes that occur at the beginning of the dark flash block and those that occur at the end.Multiple measures are calculated for the experiment, and both summarized and raw measures are shown in Figure 2 and the Supplementary Material.The summarized data is generated by merging related terms (e.g., all the "frequency" measures) together.The size of the bubble represents the percent of significant measurements in the summarized category, and the color represents the mean of the strictly standardized mean difference (SSMD) of the significant assays in that category.It is possible for some measures in the merged group to be increased (purple) and some decreased (orange), such as if a behavioral change occurs between 4 and 6 dpf, which is why it is possible to have two offset bubbles in each square.The scripts for merging these measurements and generating the summarized bubble plot graphs are available at https://github.com/thymelab/DownstreamAnalysis.Reverse Transcription PCR (RT-PCR) RNA was extracted from zebrafish embryos using the E.Z.N.A. MicroElute Total RNA Kit (Omega Bio-Tek R6834-02), and cDNA synthesis was performed with iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad #1708840).Following PCR with GoTaq polymerase (Promega M7123) and primers (Table S1), samples were run on a 4% agarose gel and the band intensities were quantified using Fiji (Schindelin et al., 2012) W., Riedel, D., von Mollard, G.F., 2000.The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex.J. Neurosci. 20, 5724-5732. doi:10.1523/JNEUROSCI.20-15-05724.2000Berns, D.S., DeNardo, L.A., Pederick, D.T., Luo, L., 2018. Teneurin-3 controls topographic circuit assembly in the hippocampus.Nature 554, 328-333.doi:10.1038/nature25463Bollmann, C., Schöning, S., Kotschnew, K., Grosse, J., Heitzig, N., Fischer von Mollard, G., 2022.Primary neurons lacking the SNAREs vti1a and vti1b show altered neuronal development.Neural Dev.17, 12. doi:10.1186/s13064-022-00168-2

Fig. 1 .
Fig. 1.Generation and analysis of zebrafish with mutations that remove conserved, developmentally regulated microexons.(A) Pipeline of the screen.Mutant lines with alternatively spliced microexons removed were generated with CRISPR/Cas9, crossed together, and sibling larvae were assessed for changes to brain morphology, brain activity, and behavioral profiling.Created with BioRender.com.(B) The amino acid sequence identity of 95 zebrafish microexons compared to mouse.(C) The amino acid sequence identity of 95 zebrafish microexons compared to mouse and divided by the sequence identity of the entire protein.(D) Gene Ontology analysis of biological processes associated with the 95 mouse microexons that are conserved in zebrafish.The analysis was completed using the PANTHER classification system.(E) Quantification of reverse transcription PCR (RT-PCR)

Fig. 2 .
Fig. 2. Larval behavioral phenotypes of zebrafish with microexons removed.(A) Summary of behavioral pipeline.(B) Baseline behavioral phenotypes for microexon mutants.The labels "1" and "2" indicate biological replicates.The data shown is for homozygous mutant larvae compared to wild-type siblings.The size of the bubble represents the percent of significant measurements in the summarized category, and the color

Frequency
[△pix] / hour represents the mean of the strictly standardized mean difference (SSMD) of the significant assays in that category.(C) Stimulus-driven behavioral phenotypes for microexon mutants.The labels "1" and "2" indicate biological replicates.The bubble size and color are calculated the same as in panel B. (D) Examples of behavioral phenotypes.The black boxes in panels B and C correspond to the selected plots.Wild-type siblings (black) are compared to the homozygous (red), and the plots show mean ± s.e.m..All N are in Table

Fig. 3 .
Fig. 3. Whole-brain activity and morphology phenotypes of zebrafish with microexons removed.(A) Summary of pErk comparisons between homozygous mutants and wild-type control siblings, where magenta represents decreased activity and green represents increased.The signal in each region was summed and divided by the region size.The N for all experiments is available in Table S2.(B) Summary of structure comparisons between homozygous mutants and wild-type control siblings, where magenta represents decreased size and green represents increased.(C) Location of major regions in the zebrafish brain based on masks from the Z-Brain atlas (Randlett et al., 2015).(D) Brain imaging for microexon mutants with repeatable brain activity phenotypes.The brain images represent the significant signal difference between homozygous and wild-type control siblings.They are shown as sum-of-slices projections (Z-and X-axes) with the white outline representing the zebrafish brain.(E) Structural phenotype of the ppp6r3 mutant, with replicates shown side-by-side.The magenta indicates decreased size.(F) Brain imaging for two microexon mutants with brain activity phenotypes that are similar for both the heterozygous and homozygous mutants.
. Data and materials availability The mutants described in the paper are available from ZIRC.Code is available from https://github.com/thymelab.Processed behavioral and imaging data is available from Zenodo under the following DOIs: 10.5281/zenodo.13137486(behavioroutputfiles),10.5281/zenodo.13138646(behaviorinputfiles),and10.5281/zenodo.13138766(brainactivitymappingstacks).AcknowledgmentsThis research was supported by a Mallinckrodt Award from the Edward Mallinckrodt Jr. Foundation (SBT).Guillermo Parada was instrumental to this work by sharing his list of conserved and developmentally regulated microexons while his study was still a preprint under review.We thank the UAB fish facility staff, the Zebrafish International Resource Center (ZIRC), particularly Andrzej Nasiadka, the Research Computing team at UAB, and the UAB Department of Neurobiology for supporting this study.We also thank the following Thyme lab members for experimental assistance or training that supported this study: Anna Moyer, Lynne Zhou, Vaishnavi Balaji, Alexia Barcus, and Sahil Malhotra.Author contributionsSBT conceived of the study, analyzed imaging and behavioral data, and wrote the manuscript.VM, JW, and CLC generated the mutant lines.CCSC and KM initiated the project and CCSC collected most of the experimental data.MESC, WCG, MCK, CLC, and EGT contributed to the experimental data in this work by genotyping and propagating zebrafish lines, running larval behavior experiments, and collecting brain imaging data.