Abstract
We evaluated the deep-sequencing (RNA-seq) data from human prostate tissue that were reported in [1] and the tRNA-derived fragments described in the original analysis. Our study of the same RNA-seq datasets reveals a considerably different pool of tRNA fragments, many of them with higher abundances than the fragments reported in [1]. We also evaluated the q-PCR approach proposed in [1]. As the approach lacks 5’-endpoint specificity, it will not estimate correctly the abundance of many of the tRFs that are present in the sampled RNA populations from human prostate tissue.
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