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CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells

Samuel A. Myers, Jason Wright, Feng Zhang, Steven A. Carr
doi: https://doi.org/10.1101/159517
Samuel A. Myers
Broad Institute of MIT and Harvard
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  • For correspondence: sammyers@broadinstitute.org
Jason Wright
Broad Institute of MIT and Harvard
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Feng Zhang
Broad Institute of MIT and Harvard
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Steven A. Carr
Broad Institute of MIT and Harvard
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Abstract

The activation or repression of a gene's expression is primarily controlled by changes in the proteins that occupy its regulatory elements. The most common method to identify proteins associated with genomic loci is chromatin immunoprecipitation (ChIP). While having greatly advanced our understanding of gene expression regulation, ChIP requires specific, high quality, IP-competent antibodies against nominated proteins, which can limit its utility and scope for discovery. Thus, a method able to discover and identify proteins associated with a particular genomic locus within the native cellular context would be extremely valuable. Here, we present a novel technology combining recent advances in chemical biology, genome targeting, and quantitative mass spectrometry to develop genomic locus proteomics, a method able to identify proteins which occupy a specific genomic locus.

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The copyright holder for this preprint is the author/funder. It is made available under a CC-BY-ND 4.0 International license.
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  • Posted July 4, 2017.

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CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells
Samuel A. Myers, Jason Wright, Feng Zhang, Steven A. Carr
bioRxiv 159517; doi: https://doi.org/10.1101/159517
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CRISPR/Cas9-APEX-mediated proximity labeling enables discovery of proteins associated with a predefined genomic locus in living cells
Samuel A. Myers, Jason Wright, Feng Zhang, Steven A. Carr
bioRxiv 159517; doi: https://doi.org/10.1101/159517

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