New Results
Single-shot super-resolution total internal reflection fluorescence microscopy
Min Guo, Panagiotis Chandris, John Paul Giannini, Adam J. Trexler, Robert Fischer, Jiji Chen, Harshad D. Vishwasrao, Ivan Rey-Suarez, Yicong Wu, Clare M. Waterman, George H. Patterson, Arpita Upadhyaya, Justin Taraska, Hari Shroff
doi: https://doi.org/10.1101/182121
Min Guo
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Panagiotis Chandris
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
John Paul Giannini
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
4Biophysics Program, University of Maryland, College Park, Maryland, USA
Adam J. Trexler
2National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Robert Fischer
2National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Jiji Chen
3Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, Maryland, USA
Harshad D. Vishwasrao
3Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, Maryland, USA
Ivan Rey-Suarez
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
4Biophysics Program, University of Maryland, College Park, Maryland, USA
Yicong Wu
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Clare M. Waterman
2National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
George H. Patterson
5Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Arpita Upadhyaya
6Department of Physics and Institute for Physical Science and Technology, University of Maryland, College Park, Maryland, USA
Justin Taraska
2National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Hari Shroff
1Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
3Advanced Imaging and Microscopy Resource, National Institutes of Health, Bethesda, Maryland, USA
6Department of Physics and Institute for Physical Science and Technology, University of Maryland, College Park, Maryland, USA
Abstract
We demonstrate a simple method for combining instant structured illumination microscopy (SIM) with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF (down to 115 +/-13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-resolution imaging in close proximity to the coverslip surface.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted August 29, 2017.
Single-shot super-resolution total internal reflection fluorescence microscopy
Min Guo, Panagiotis Chandris, John Paul Giannini, Adam J. Trexler, Robert Fischer, Jiji Chen, Harshad D. Vishwasrao, Ivan Rey-Suarez, Yicong Wu, Clare M. Waterman, George H. Patterson, Arpita Upadhyaya, Justin Taraska, Hari Shroff
bioRxiv 182121; doi: https://doi.org/10.1101/182121
Single-shot super-resolution total internal reflection fluorescence microscopy
Min Guo, Panagiotis Chandris, John Paul Giannini, Adam J. Trexler, Robert Fischer, Jiji Chen, Harshad D. Vishwasrao, Ivan Rey-Suarez, Yicong Wu, Clare M. Waterman, George H. Patterson, Arpita Upadhyaya, Justin Taraska, Hari Shroff
bioRxiv 182121; doi: https://doi.org/10.1101/182121
Subject Area
Subject Areas
- Biochemistry (11715)
- Bioengineering (8723)
- Bioinformatics (29128)
- Biophysics (14935)
- Cancer Biology (12049)
- Cell Biology (17359)
- Clinical Trials (138)
- Developmental Biology (9406)
- Ecology (14144)
- Epidemiology (2067)
- Evolutionary Biology (18268)
- Genetics (12221)
- Genomics (16767)
- Immunology (11843)
- Microbiology (28014)
- Molecular Biology (11560)
- Neuroscience (60810)
- Paleontology (450)
- Pathology (1864)
- Pharmacology and Toxicology (3231)
- Physiology (4940)
- Plant Biology (10384)
- Synthetic Biology (2878)
- Systems Biology (7333)
- Zoology (1642)