Abstract
Summary Micrococcal nuclease digestion followed by deep sequencing (MNase-seq) is the most used method to investigate nucleosome organization on a genome-wide scale. We present plot2DO, a software package for creating 2D occupancy plots, which allows biologists to evaluate the quality of MNase-seq data and to visualize the distribution of nucleosomes near the functional regions of the genome (e.g. gene promoters, origins of replication, etc.).
Availability And Implementation The plot2DO open source package is freely available on GitHub at https://github.com/rchereji/plot2DO under the MIT license.
Contact razvan.chereji{at}nih.gov
Copyright
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