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Rif1 prolongs the embryonic S phase at the Drosophila mid-blastula transition

Charlers A Seller, View ORCID ProfilePatrick H O'Farrell
doi: https://doi.org/10.1101/231357
Charlers A Seller
University of California, San Francisco
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Patrick H O'Farrell
University of California, San Francisco
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  • ORCID record for Patrick H O'Farrell
  • For correspondence: ofarrell@cgl.ucsf.edu
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Abstract

In preparation for dramatic morphogenetic events of gastrulation, rapid embryonic cell cycles slow at the Mid-Blastula Transition, MBT. In Drosophila melanogaster embryos, downregulation of Cdk1 activity initiates this slowing by delaying replication of satellite sequences and extending S phase. We found that Cdk1 inhibited the chromatin association of Rif1, a candidate repressor of replication. Furthermore, Rif1 bound selectively to satellite sequences following Cdk1 downregulation at the MBT. In the next S phase, Rif1 dissociated from different satellites in an orderly schedule that anticipated their replication. Rif1 lacking potential phosphorylation sites failed to dissociate and dominantly prevented completion of replication. Loss of Rif1 in mutant embryos shortened the post-MBT S phase, and rescued embryonic cell cycles disrupted by depletion of the S phase-promoting kinase, Cdc7. Thus, Drosophila Rif1 mediates the MBT extension of S phase and functionally interacts with S phase promoting kinases to introduce a replication-timing program.

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The copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license.
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  • Posted December 14, 2017.

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Rif1 prolongs the embryonic S phase at the Drosophila mid-blastula transition
Charlers A Seller, Patrick H O'Farrell
bioRxiv 231357; doi: https://doi.org/10.1101/231357
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Rif1 prolongs the embryonic S phase at the Drosophila mid-blastula transition
Charlers A Seller, Patrick H O'Farrell
bioRxiv 231357; doi: https://doi.org/10.1101/231357

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