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Phyla-specific Correlated Dynamics in Tropomyosin

Jose K. James, View ORCID ProfileVikas Nanda
doi: https://doi.org/10.1101/301028
Jose K. James
Center for Advanced Biotechnology and Medicine
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Vikas Nanda
Center for Advanced Biotechnology and Medicine
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  • ORCID record for Vikas Nanda
  • For correspondence: viknanda@gmail.com
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Abstract

Tropomyosin (Tpm) is a continuous α-helical coiled-coil homodimer that regulates actinomyosin interactions in muscle. We examined extended molecular simulations of four Tpms, two from the vertebrate phylum Chordata (rat and pig), and two from the invertebrate Arthropoda (shrimp and lobster), and found that despite extensive sequence and structural homology across metazoans, dynamic behavior - particularly long range structural fluctuations - were clearly distinct between phyla. Vertebrate Tpms were flexible and sampled complex, multi-state conformational landscapes. Invertebrate Tpms were rigid, sampling highly constrained harmonic landscapes. Filtering of trajectories by PCA into essential subspaces showed significant overlap within but not between phyla. In vertebrate Tpms, hinge-regions decoupled long-range inter-helical motions and suggested distinct domains. In contrast, crustacean Tpms lacked significant long range dynamic correlations - behaving more like a single rigid rod. Although Tpm sequence and structure has highly conserved over the last 0.6-billion years since the split of ancestral bilateria into protostomes and deuterostomes, divergence seems to have occurred at the level of long-range correlated dynamics, reflecting adaptations to phyla-specific requirements of actin binding and muscle contraction.

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The copyright holder for this preprint is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
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  • Posted April 13, 2018.

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Phyla-specific Correlated Dynamics in Tropomyosin
Jose K. James, Vikas Nanda
bioRxiv 301028; doi: https://doi.org/10.1101/301028
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Phyla-specific Correlated Dynamics in Tropomyosin
Jose K. James, Vikas Nanda
bioRxiv 301028; doi: https://doi.org/10.1101/301028

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