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Actin filaments regulate microtubule growth at the centrosome.

View ORCID ProfileManuel Thery, Daisuke Inoue, View ORCID ProfileDorian Obino, Francesca Farina, Jérémie Gaillard, Christophe Guérin, Laurent Blanchoin, Ana-Maria Lennon-Duménil
doi: https://doi.org/10.1101/302190
Manuel Thery
CEA, Hopital Saint-Louis, Paris;
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  • For correspondence: manuel.thery@cea.fr
Daisuke Inoue
CEA, BIG, Grenoble;
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Dorian Obino
Institut Curie, CNRS;
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Francesca Farina
CEA, BIG, Grenoble;
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Jérémie Gaillard
CNRS UMR5168/CEA/INRA/Grenoble University;
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Christophe Guérin
CEA, BIG, Grenoble;
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Laurent Blanchoin
CEA Grenoble
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Ana-Maria Lennon-Duménil
Institut Curie, CNRS;
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Abstract

The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here we show that increasing densities of actin filaments at the centrosome of lymphocytes were correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in centrosomal-actin disassembly and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. The two assays demonstrated that actin filaments perturb microtubule growth by steric hindrance. Finally, we showed that cell adhesion and spreading leads to lower densities of centrosomal actin thus resulting in higher microtubule growth. Hence we propose a novel mechanism by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture.

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The copyright holder for this preprint is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
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  • Posted April 16, 2018.

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Actin filaments regulate microtubule growth at the centrosome.
Manuel Thery, Daisuke Inoue, Dorian Obino, Francesca Farina, Jérémie Gaillard, Christophe Guérin, Laurent Blanchoin, Ana-Maria Lennon-Duménil
bioRxiv 302190; doi: https://doi.org/10.1101/302190
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Actin filaments regulate microtubule growth at the centrosome.
Manuel Thery, Daisuke Inoue, Dorian Obino, Francesca Farina, Jérémie Gaillard, Christophe Guérin, Laurent Blanchoin, Ana-Maria Lennon-Duménil
bioRxiv 302190; doi: https://doi.org/10.1101/302190

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