New Results
An optimized toolkit for precision base editing
Maria Paz Zafra, Emma M Schatoff, Alyna Katti, Miguel Foronda, Marco Breinig, Anabel Y. Schweitzer, Amber Simon, Teng Han, Sukanya Goswami, Emma Montgomery, Jordana Thibado, Francisco J. Sánchez-Rivera, Junwei Shi, Christopher R Vakoc, Scott W Lowe, Darjus F. Tschaharganeh, Lukas E Dow
doi: https://doi.org/10.1101/303131
Maria Paz Zafra
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
Emma M Schatoff
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
2Weill Cornell / Rockefeller / Sloan Kettering Tri-I MD-PhD program, New York, NY
Alyna Katti
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
3Weill Cornell Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY
Miguel Foronda
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
Marco Breinig
4Helmholtz-University Group “Cell Plasticity and Epigenetic Remodeling”, German Cancer Research Center (DKFZ) & Institute of Pathology University Hospital, 69120 Heidelberg, Germany
Anabel Y. Schweitzer
4Helmholtz-University Group “Cell Plasticity and Epigenetic Remodeling”, German Cancer Research Center (DKFZ) & Institute of Pathology University Hospital, 69120 Heidelberg, Germany
Amber Simon
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
Teng Han
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
3Weill Cornell Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY
Sukanya Goswami
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
Emma Montgomery
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
Jordana Thibado
3Weill Cornell Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY
Francisco J. Sánchez-Rivera
5Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY
Junwei Shi
6Cold Spring Harbor Laboratory, New York, NY
7Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, PA
Christopher R Vakoc
6Cold Spring Harbor Laboratory, New York, NY
Scott W Lowe
5Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY
8Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, NY
Darjus F. Tschaharganeh
4Helmholtz-University Group “Cell Plasticity and Epigenetic Remodeling”, German Cancer Research Center (DKFZ) & Institute of Pathology University Hospital, 69120 Heidelberg, Germany
Lukas E Dow
1Sandra and Edward Meyer Cancer Center, Department of Medicine, Weill Cornell Medicine, New York, NY
3Weill Cornell Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY
9Department of Biochemistry, Weill Cornell Medicine, New York, NY
Abstract
CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single base pair resolution. By re-engineering both DNA and protein sequences, we developed a collection of constitutive and inducible base editing vector systems that dramatically improve the ease and efficiency by which single nucleotide variants can be created. This new toolkit is effective in a wide range of model systems, and provides a means for efficient in vivo somatic base editing.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted April 17, 2018.
An optimized toolkit for precision base editing
Maria Paz Zafra, Emma M Schatoff, Alyna Katti, Miguel Foronda, Marco Breinig, Anabel Y. Schweitzer, Amber Simon, Teng Han, Sukanya Goswami, Emma Montgomery, Jordana Thibado, Francisco J. Sánchez-Rivera, Junwei Shi, Christopher R Vakoc, Scott W Lowe, Darjus F. Tschaharganeh, Lukas E Dow
bioRxiv 303131; doi: https://doi.org/10.1101/303131
An optimized toolkit for precision base editing
Maria Paz Zafra, Emma M Schatoff, Alyna Katti, Miguel Foronda, Marco Breinig, Anabel Y. Schweitzer, Amber Simon, Teng Han, Sukanya Goswami, Emma Montgomery, Jordana Thibado, Francisco J. Sánchez-Rivera, Junwei Shi, Christopher R Vakoc, Scott W Lowe, Darjus F. Tschaharganeh, Lukas E Dow
bioRxiv 303131; doi: https://doi.org/10.1101/303131
Subject Area
Subject Areas
- Biochemistry (11718)
- Bioengineering (8724)
- Bioinformatics (29132)
- Biophysics (14936)
- Cancer Biology (12051)
- Cell Biology (17360)
- Clinical Trials (138)
- Developmental Biology (9406)
- Ecology (14146)
- Epidemiology (2067)
- Evolutionary Biology (18269)
- Genetics (12223)
- Genomics (16768)
- Immunology (11844)
- Microbiology (28016)
- Molecular Biology (11560)
- Neuroscience (60822)
- Paleontology (450)
- Pathology (1864)
- Pharmacology and Toxicology (3231)
- Physiology (4940)
- Plant Biology (10401)
- Synthetic Biology (2878)
- Systems Biology (7333)
- Zoology (1642)