Abstract
Recently engineered CRISPR base editors have opened unique avenues for scar-free genome-wide mutagenesis. Here, we describe a comprehensive computational workflow called beditor that can be broadly adapted for designing guide RNA libraries to be used for CRISPR base editing. The computational framework allows users to assess editing possibilities using a range of CRISPR base editors, PAM recognition sequences and the genome of any species. Additionally, potential editing efficiencies of the designed guides are evaluated in terms of an a priori estimates, through a specifically designed beditor scoring system.
Copyright
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