ABSTRACT
Potato virus Y (PVY) is a major potato pathogen that causes annual losses of billions of dollars. Control of its transmission requires extensive use of environmentally damaging insecticides. Rysto confers extreme resistance (ER) to PVY and is a valuable trait in resistance breeding programs. We isolated Rysto using Resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences Single-Molecule Real Time Sequencing). Rysto encodes a nucleotide binding-leucine rich repeat (NLR) protein with an N-terminal TIR domain, and is sufficient for PVY perception and extreme resistance in transgenic potato plants. We investigated the requirements for Rysto-dependent extreme resistance, and showed that Rysto function is temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.
Author contributions:
M.G-B., K.W and J.H. performed most of the experimental work, data analyses, and writing; A. W., I.W-F., H.J., K.S. and K.M performed the research; J.J. and W.M. edited the article; J.H. supervised the work and assisted with writing.
Footnotes
Funding information: Research was supported by OPUS 8 (2014/15/B/NZ9/04384) and OPUS 14 (2017/27/B/NZ9/01803) grants from the National Science Centre, Poland, to Jacek Hennig. Kamil Witek was supported by BBSRC grants BB/L009293/1 and BB/P021646/1 to Jonathan Jones.