TY - JOUR T1 - First report and multilocus genotyping of <em>Enterocytozoon bieneusi</em> from Tibetan pigs in southwestern China JF - bioRxiv DO - 10.1101/327767 SP - 327767 AU - Run Luo AU - Leiqiong Xiang AU - Haifeng Liu AU - Zhijun Zhong AU - Li Liu AU - Lei Deng AU - Yuan Song AU - Ling Liu AU - Xiangming Huang AU - Ziyao Zhou AU - Hualin Fu AU - Yan Luo AU - Guangneng Peng Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/05/21/327767.abstract N2 - Enterocytozoon bieneusi is a common intestinal pathogen and a major cause of diarrhea and enteric diseases in a variety of animals. While the E. bieneusi genotype has become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. E. bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan and 26.7% in Qionglai. Age group demonstrated the prevalence of E. bieneusi range from 24.4%(aged 0 to 1 years) to 44.4%(aged 1 to 2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n=58), Henan-IV (n=24) and two novel genotypes, SCT01 and SCT02 (one of each). Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) revealed 47, 48, 23 and 47 positive specimens were successfully sequenced, and identified ten, ten, five and five genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic data for preventing and controlling infections. ER -