RT Journal Article SR Electronic T1 Extracellular Electron Transfer by Shewanella oneidensis Controls Pd Nanoparticle Phenotype JF bioRxiv FD Cold Spring Harbor Laboratory SP 327460 DO 10.1101/327460 A1 Christopher M. Dundas A1 Austin J. Graham A1 Dwight K. Romanovicz A1 Benjamin K. Keitz YR 2018 UL http://biorxiv.org/content/early/2018/05/21/327460.abstract AB Biological production of inorganic materials is impeded by relatively few organisms possessing genetic and metabolic linkage to material properties. The physiology of electroactive bacteria is intimately tied to inorganic transformations, which makes genetically tractable and well-studied electrogens, such as Shewanella oneidensis, attractive hosts for material synthesis. Notably, this species is capable of reducing a variety of transition-metal ions into functional nanoparticles, but exact mechanisms of nanoparticle biosynthesis remain ill-defined. We report two key factors of extracellular electron transfer by S. oneidensis, the outer membrane cytochrome, MtrC, and soluble redox shuttles (flavins), that affect Pd nanoparticle formation. Changes in the expression and availability of these electron transfer components drastically modulated particle phenotype, including particle synthesis rate, structure, and cellular localization. These relationships may serve as the basis for biologically tailoring Pd nanoparticle catalysts and could potentially be used to direct the biogenesis of other metal nanomaterials.