RT Journal Article
SR Electronic
T1 Existence and functions of hypothalamic kisspeptin neuropeptide signaling system in a non-chordate deuterostome species
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 851261
DO 10.1101/851261
A1 Tianming Wang
A1 Zheng Cao
A1 Zhangfei Shen
A1 Jingwen Yang
A1 Xu Chen
A1 Zhen Yang
A1 Ke Xu
A1 Xiaowei Xiang
A1 Qiuhan Yu
A1 Yimin Song
A1 Weiwei Wang
A1 Yanan Tian
A1 Lina Sun
A1 Libin Zhang
A1 Su tGuo
A1 Naiming Zhou
YR 2019
UL http://biorxiv.org/content/early/2019/11/21/851261.abstract
AB The kisspeptin (Kp) system is a central modulator of the hypothalamic-pituitary-gonadal axis in vertebrates. Its existence outside the vertebrate lineage remains largely unknown. Here we report the identification and characterization of Kp system in the sea cucumber Apostichopus japonicus. The gene encoding the Kp precursor, generates two mature neuropeptides, AjKiss1a and AjKiss1b. The Kp receptors, AjKissR1 and AjKissR2, are strongly activated by synthetic A. japonicus and vertebrate Kps, triggering a rapid intracellular mobilization of Ca2+, followed by receptor internalization. AjKissR1 and AjKissR2 share similar intracellular signaling pathways via Gαq/PLC/PKC/MAPK cascade, when activated by C-terminal decapeptide (AjKiss1b-10). The A. japonicus Kp system functions in mutiple tissues which are closely related to reproduction and metabolism. Overall, our findings uncover for the first time, to our knowledge, the existence and function of the Kp system in a non-chordate species and provide new evidence to support the ancient origin of the hypothalamic neurosecretory system.